[1]马永梅,樊 荣.传染性单核细胞增多症患儿血清Toll样受体2、Toll样受体9与外周血T淋巴细胞亚群变化及临床意义[J].陕西医学杂志,2022,51(11):1416-1419.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.023]
　MA Yongmei,FAN Rong.Changes and clinical significance of serum TLR2,TLR9 and peripheral blood T lymphocyte subsets in children with infectious mononucleosis[J].,2022,51(11):1416-1419.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.023]
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传染性单核细胞增多症患儿血清Toll样受体2、Toll样受体9与外周血T淋巴细胞亚群变化及临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年11期
页码:
1416-1419
栏目:
临床研究
出版日期:
2022-11-05

文章信息/Info

Title:
Changes and clinical significance of serum TLR2,TLR9 and peripheral blood T lymphocyte subsets in children with infectious mononucleosis
作者:
马永梅; 樊 荣
(宝鸡市妇幼保健院儿科,陕西 宝鸡 721000)
Author(s):
MA Yongmei; FAN Rong
(Department of Pediatrics,Baoji Maternal and Child Health Hospital,Baoji 721000,China)
关键词:
传染性单核细胞增多症;  儿童;  Toll样受体2;  Toll样受体9;  T淋巴细胞亚群;  EB病毒脱氧核糖核酸
Keywords:
Infectious mononucleosis;  Children;  Toll-like receptor 2;  Toll-like receptor 9;  T lymphocyte subsets;  EBV-DNA
分类号:
R 725.1
DOI:
DOI:10.3969/j.issn.1000-7377.2022.11.023
文献标志码:
A
摘要:
目的:分析传染性单核细胞增多症(IM)患儿血清Toll样受体2(TLR2)、TLR9与外周血T淋巴细胞亚群变化及临床意义。方法:回顾性分析106例IM患儿临床资料。记录并比较IM患儿急性期与恢复期血清TLR2、TLR9及外周血T淋巴细胞亚群(CD3+、CD4+、CD8+、CD4+/CD8+)变化情况。根据治疗7 d时外周血EB病毒脱氧核糖核酸(EBV-DNA)转阴情况将IM患儿分为转阴组和未转阴组,比较两组一般资料,以及治疗7 d时血清TLR2、TLR9及外周血T淋巴细胞亚群表达水平。分析治疗7 d时EBV-DNA载量与血清TLR2、TLR9及外周血T淋巴细胞亚群表达水平的关系。结果: IM患儿急性期血清TLR2、TLR9表达水平高于恢复期,外周血CD3+、CD8+表达水平高于恢复期,外周血CD4+及CD4+/CD8+表达水平则低于恢复期(均P<0.05)。治疗7 d时,106例IM患儿EBV-DNA转阴82例(77.36%),纳入转阴组; 未转阴24例(22.64%),纳入未转阴组。两组患儿性别、入院前病程及治疗前白细胞计数比较差异无统计学意义(均P>0.05); 未转阴组年龄低于转阴组,治疗前EBV-DNA载量高于转阴组(均P<0.05)。治疗7 d时,未转阴组血清TLR2、TLR9表达水平高于转阴组,外周血CD3+、CD8+表达水平高于转阴组,外周血CD4+及CD4+/CD8+低于转阴组(均P<0.05)。经Pearson相关性分析,发现EBV-DNA载量与血清TLR2、TLR9及外周血CD3+、CD8+表达水平呈正相关(r=0.381、0.362、0.319、0.347,均P<0.05),与外周血CD4+及CD4+/CD8+呈负相关(r=-0.360、-0.395,均P<0.05)。结论:血清TLR2、TLR9及外周血CD3+、CD4+、CD8+、CD4+/CD8+表达水平随IM患儿病情转归而变化,积极监测上述指标可辅助临床判断病情。
Abstract:
Objective:To analyze the changes and clinical significance of serum Toll-like receptor 2(TLR2),TLR9 and peripheral blood T lymphocyte subsets in children with infectious mononucleosis(IM).Methods:The clinical data of 106 children with IM were retrospectively analyzed.The changes of serum TLR2,TLR9 and peripheral blood T lymphocyte subsets(CD3+、CD4+、CD8+、CD4+/CD8+)were recorded and compared between acute phase and convalescent phase.According to the negative conversion of peripheral blood EB virus deoxyribonucleic acid(EBV-DNA)of children with IM after 7 days of treatment,they were divided into negative conversion group and non-negative conversion group.The general data,the expression levels of serum TLR2,TLR9 and peripheral blood T lymphocyte subsets were compared between the two groups after 7 days of treatment.Pearson correlation analysis was used to evaluate the relationship between EBV-DNA load and expression levels of serum TLR2,TLR9 and peripheral blood T lymphocyte subsets after 7 days of treatment.Results:The expression levels of serum TLR2,TLR9 and peripheral blood CD3+ and CD8+ in acute phase were higher than those in convalescent phase,while the peripheral blood CD4+ and CD4+/CD8+ were lower than those in convalescent phase(all P<0.05).After 7 days of treatment,among 106 children with IM,82 cases(77.36%)with negative conversion were included in negative conversion group,and 24 cases(22.64%)without negative conversion were included in non-negative conversion group.There was no significant difference in gender,course of disease before admission and white blood cell count before treatment between two groups(all P>0.05).The age in non-negative conversion group was lower than that in negative conversion group,while the EBV-DNA load before treatment was higher than that in negative conversion group(all P<0.05).After 7 days of treatment,the expression levels of serum TLR2,TLR9 and peripheral blood CD3+ and CD8+ in non-negative conversion group were higher than those in negative conversion group,while the peripheral blood CD4+ and CD4+/CD8+ were lower than those in negative conversion group(all P<0.05).Pearson correlation analysis showed that EBV-DNA load was positively correlated with expression levels of serum TLR2,TLR9 and peripheral blood CD3+ and CD8+(r=0.381,0.362,0.319,0.347,all P<0.05),and was negatively correlated with peripheral blood CD4+ and CD4+/CD8+(r=-0.360,-0.395,all P<0.05).Conclusion:The expression levels of serum TLR2,TLR9 and peripheral blood CD3+,CD4+,CD8+ and CD4+/CD8+ change with the outcome of children with IM.Active monitoring of the above indexes can assist clinical judgment.

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备注/Memo

备注/Memo:
基金项目:陕西省中医药管理局科研课题(2021-GJ-LC021)
更新日期/Last Update: 2022-11-09