[1]李宝福,刘柏麟,袁 兴,等.辅酶Q10对小鼠脑出血后神经保护作用的实验研究[J].陕西医学杂志,2022,51(11):1337-1340.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.004]
　LI Baofu,LIU Bolin,YUAN Xing,et al.Neuroprotective effect of coenzyme Q10 on intracerebral hemorrhage in mice[J].,2022,51(11):1337-1340.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.004]
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辅酶Q10对小鼠脑出血后神经保护作用的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年11期
页码:
1337-1340
栏目:
基础研究
出版日期:
2022-11-05

文章信息/Info

Title:
Neuroprotective effect of coenzyme Q10 on intracerebral hemorrhage in mice
作者:
李宝福; 刘柏麟; 袁 兴; 郝璞珩; 邓齐奇; 孙皖秦
(西安国际医学中心医院神经外科,陕西 西安 710100)
Author(s):
LI Baofu; LIU Bolin; YUAN Xing; HAO Puheng; DENG Qiqi; SUN Wanqin
(Department of Neurosurgery,Xi'an International Medical Center Hospital,Xi'an 710100,China)
关键词:
脑出血;  辅酶Q10;  炎症因子;  神经保护;  凋亡;  炎性反应
Keywords:
Intracerebral hemorrhage;  Coenzyme Q10;  Inflammatory factors;  Neuroprotection;  Apoptosis;  Inflammatory reaction
分类号:
R 651.1
DOI:
DOI:10.3969/j.issn.1000-7377.2022.11.004
文献标志码:
A
摘要:
目的:研究辅酶Q10(CoQ10)对脑出血(ICH)小鼠的神经功能、周围组织水肿、炎症因子水平和细胞凋亡的影响。方法:将54只C57WT小鼠随机分为Sham组、ICH组、CoQ10组,采用 Ⅳ型胶原酶注射到基底神经节的方法建立脑出血模型,CoQ10组小鼠在脑出血模型建立6 h后,给予CoQ10灌胃处理。于造模后7 d时对所有小鼠进行mNSS评分评估其神经功能,并用公式计算小鼠脑水肿程度; 于3 d时采用酶联免疫吸附试验检测炎症因子白介素6(IL-6)及肿瘤坏死因子-α(TNF-α)的表达水平,TUNEL染色法检测细胞凋亡水平。结果:CoQ10治疗降低了脑出血小鼠造模7 d后的mNSS评分(P<0.05),脑组织水含量计算的结果也显示ICH组脑水肿程度加重(P<0.001),而CoQ10组可减轻小鼠的脑水肿(P<0.01)。与ICH组相比,经CoQ10处理可以显著降低炎症因子的表达水平(P<0.01)及减少细胞凋亡水平(P<0.001)。结论:CoQ10可减轻脑出血小鼠神经功能障碍、降低脑水肿程度、减少炎症因子产生、改善细胞凋亡。
Abstract:
Objective:To study the effects of coenzyme Q10(CoQ10)on nerve function,surrounding tissue edema,inflammatory factor levels and apoptosis of intracerebral hemorrhage(ICH)mice.Methods:Fifty-four C57 WT mice were randomly divided into Sham group,ICH group and CoQ10 group.ICH group and CoQ10 group were injected with type Ⅳ collagenase into basal ganglia to establish ICH model.CoQ10 group was given CoQ10 intragastric treatment 6 hours after the establishment of ICH model.At 7 days after modeling,all mice were evaluated by mNSS score,and the degree of cerebral edema was calculated by formula.At 3 days,the expression levels of inflammatory factors were detected by enzyme-linked immunosorbent assay,and the apoptosis level was detected by TUNEL staining.Results:CoQ10 treatment reduced the mNSS score(P<0.05),the calculation results of brain water content also showed that the degree of cerebral edema increased in the ICH group(P<0.001),while CoQ10 reduced cerebral edema in mice(P<0.01).Compared with ICH group,CoQ10 could significantly reduce the expression level of inflammatory factors and decrease apoptosis levels(all P<0.001).Conclusion:CoQ10 can relieve the neurological dysfunction,reduce the degree of cerebral edema,reduce the production of inflammatory factors and improve cell apoptosis in mice with ICH.

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更新日期/Last Update: 2022-11-09