[1]芦 兰,王 鑫,张瑞琦,等.酒精性心肌病大鼠模型中凝集素样氧化低密度脂蛋白受体-1表达与细胞凋亡相关蛋白Bax、Cleaved caspase-3表达关系研究[J].陕西医学杂志,2022,51(10):1201-1205.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.006]
　LU Lan,WANG Xin,ZHANG Ruiqi,et al.Relationship between expression of LOX-1 and apoptosis-related proteins Bax,Cleaved caspase-3 in rat model of alcoholic cardiomyopathy[J].,2022,51(10):1201-1205.[doi:DOI:10.3969/j.issn.1000-7377.2022.10.006]
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酒精性心肌病大鼠模型中凝集素样氧化低密度脂蛋白受体-1表达与细胞凋亡相关蛋白Bax、Cleaved caspase-3表达关系研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年10期
页码:
1201-1205
栏目:
基础研究
出版日期:
2022-10-05

文章信息/Info

Title:
Relationship between expression of LOX-1 and apoptosis-related proteins Bax,Cleaved caspase-3 in rat model of alcoholic cardiomyopathy
作者:
芦 兰1; 2; 王 鑫2; 张瑞琦1; 2; 赵 伟2; 袁 博2; 张一凡2; 冯占斌2
(1.西安医学院,陕西 西安 710021; 2.西安市第九医院,陕西 西安 710054)
Author(s):
LU Lan; WANG Xin; ZHANG Ruiqi; ZHAO Wei; YUAN Bo; ZHANG Yifan; FENG Zhanbin
(Xi'an Medical College,Xi'an 710021,China)
关键词:
酒精性心肌病;  细胞凋亡;  凝集素样氧化低密度脂蛋白受体-1;  Bax;  Cleaved caspase-3
Keywords:
Alcoholic myocardiopathy;  Apoptosis;  Lectin-like oxidized low-density lipoprotein receptor-1;  Bax;  Cleaved caspase-3
分类号:
R 542.2
DOI:
DOI:10.3969/j.issn.1000-7377.2022.10.006
文献标志码:
A
摘要:
目的:分析酒精性心肌病(ACM)大鼠模型中凝集素样氧化低密度脂蛋白受体-1(LOX-1)的表达情况及与细胞凋亡相关蛋白Bax、Cleaved caspase-3表达量变化的相关性。方法:选用2周龄的健康雄性SD大鼠9只,随机分为三组:①正常对照组(Blank组); ②酒精性心肌病组(ACM组); ③酒精性心肌病+shRNA干预组(ACM+shRNA组),采用实时荧光定量PCR(qRT-PCR)、蛋白质印迹法(Western blot)分别在基因及蛋白水平检测LOX-1、Bax、Cleaved caspase-3的表达量并采用Pearson检验分析其相关性。 结果:与Blank组相比,ACM组心肌组织中LOX-1、Bax、Cleaved caspase-3的表达量显著增加(P<0.01); 与ACM组比较,ACM+shRNA组心肌组织中LOX-1、Bax、Cleaved caspase-3的表达量明显减少(均P<0.01); 相关性分析提示LOX-1的表达量与Bax、Cleaved caspase-3的表达量呈正相关(r=0.323、0.370, 均P<0.05)。结论: 酒精性心肌病大鼠模型心肌组织中LOX-1的表达量升高,且与Bax、Cleaved caspase-3的表达量呈正相关; LOX-1可能通过上调Bax、Cleaved caspase-3的表达参与酒精诱导心肌细胞凋亡的过程。
Abstract:
Objective:To analyze the relationship between expression of LOX-1 and apoptosis-related proteins Bax,Cleaved caspase-3 in rat model of alcoholic cardiomyopathy.Methods:Nine healthy male SD rats at 2-week-old were selected and randomly divided into Blank group,ACM group and ACM+shRNA group(adenovirus-associated shRNA was injected intravenously on the basis of ACM group).The expression of LOX-1,Bax and Cleaved caspase-3 was detected by qRT-PCR and Western blot,and the correlation was analyzed by Pearson test.Results:Compared with the Blank group,the expressions of LOX-1,Bax and Cleaved caspase-3 were significantly increased in ACM group(all P<0.01).Compared with the ACM group,the expressions of LOX-1,Bax and Cleaved caspase-3 were significantly decreased in the ACM+shRNA group(all P<0.01).Correlation analysis suggested that LOX-1 expression was positively correlated with the Bax and Cleaved caspase-3 expression(r=0.323,0.370,all P<0.05).Conclusion:The expression level of LOX-1 was increased in alcoholic cardiomyopathy rat model,and was positively correlated with the expression level of Bax and Cleaved caspase-3.LOX-1 may be involved in alcohol-induced apoptosis of myocardial cells by up-regulating the expression of Bax and Cleaved caspase-3.

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备注/Memo

备注/Memo:
基金项目:西安市科技计划项目(21YXYJ0066)
更新日期/Last Update: 2022-10-07