[1]刘新艳,王媛媛,刘娜娜,等.谷氨酰胺代谢对人甲状腺癌细胞系体外增殖能力影响的实验研究[J].陕西医学杂志,2022,51(9):1153-1157.[doi:DOI:10.3969/j.issn.1000-7377.2022.09.026]
　LIU Xinyan,WANG Yuanyuan,LIU Nana,et al.Effects of glutamine metabolism on proliferation of human thyroid cancer cell lines in vitro[J].,2022,51(9):1153-1157.[doi:DOI:10.3969/j.issn.1000-7377.2022.09.026]
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谷氨酰胺代谢对人甲状腺癌细胞系体外增殖能力影响的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年9期
页码:
1153-1157
栏目:
临床病理
出版日期:
2022-09-05

文章信息/Info

Title:
Effects of glutamine metabolism on proliferation of human thyroid cancer cell lines in vitro
作者:
刘新艳1; 王媛媛1; 刘娜娜2; 王 荣2
(1.榆林市第一医院内分泌科,陕西 榆林 719000; 2.榆林市第一医院全科医学科,陕西 榆林 719000)
Author(s):
LIU Xinyan; WANG Yuanyuan; LIU Nana; WANG Rong
(Department of Endocrinology,the First Hospital of Yulin,Yulin 719000,China)
关键词:
甲状腺癌细胞系;  谷氨酰胺代谢;  增殖能力;  克隆形成能力;  三磷酸腺苷;  细胞凋亡
Keywords:
Thyroid cancer cell lines;  Glutamine metabolism;  Proliferation ability;  Clone formation ability;  ATP;  Apoptosis
分类号:
R 736.1
DOI:
DOI:10.3969/j.issn.1000-7377.2022.09.026
文献标志码:
A
摘要:
目的:探讨谷氨酰胺(Gln)代谢对人甲状腺癌细胞系体外增殖能力的影响。方法:体外用含不同浓度Gln培养基培养人甲状腺癌细胞系C643、IHH4、8505C、8305C、K1和TPC-1。通过噻唑蓝(MTT)及平板克隆形成实验评价Gln对甲状腺癌细胞体外增殖及克隆形成能力的影响。采用流式细胞仪检测Gln对甲状腺癌细胞凋亡的影响。利用三磷酸腺苷(ATP)检测试剂盒检测不同浓度Gln培养条件下甲状腺癌细胞ATP生成能力。结果:与含有Gln的培养基相比,不含Gln培养基培养的6株甲状腺癌细胞的增殖活性、克隆形成能力、ATP生成能力明显降低,细胞凋亡明显增加(均P<0.05)。结论:甲状腺癌细胞体外生长依赖Gln,剥夺Gln后甲状腺癌细胞体外增殖能力受到抑制,细胞凋亡增加。Gln代谢可能成为甲状腺癌尤其是未分化甲状腺癌的潜在治疗靶点。
Abstract:
Objective:To investigate the effect of glutamine(Gln)metabolism on proliferation of human thyroid cancer cell lines in vitro.Methods:Human thyroid cancer cell lines C643,IHH4,8505C,8305C,K1 and TPC-1 were cultured with different concentrations of Gln in vitro.The influence of glutamine on the proliferation and colony formation of thyroid cancer cells were evaluated by MTT and colony formation trials.The effect of Gln on apoptosis of thyroid cancer cells was measured by flow cytometry.ATP production capacity of thyroid cancer cells cultured with different concentrations of Gln was determined by using ATP assay kits.Results:Compared with the culture medium containing Gln,the proliferative activity,clone formation ability,ATP production ability of the six thyroid cancer cell lines cultured in the Gln-free culture medium were decreased and the apoptosis was increased(all P<0.05).Conclusion:The growth of thyroid cancer cells is dependent on Gln in vitro.After deprivation of Gln,the proliferation of thyroid cancer cells in vitro is inhibited and apoptosis is increased.Gln metabolism may become a potential therapeutic target for thyroid cancer,especially anaplastic thyroid cancer.

参考文献/References:

[1] Sung H,Ferlay J,Siegel RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer J Clin,2021,71(3):209-249.
[2] Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66:115-132.
[3] Wei W,Zeng H,Zheng R,et al.Cancer registration in China and its role in cancer prevention and control[J].Lancet Oncol,2020,21(7):e342-e349.
[4] Wang J,Yu F,Shang Y,et al.Thyroid cancer:Incidence and mortality trends in China,2005-2015[J].Endocrine,2020,68(1):163-173.
[5] Cabanillas ME,McFadden DG,Durante C.Thyroid cancer[J].Lancet,2016,388(10061):2783-2795.
[6] Giuffrida D,Gharib H.Anaplastic thyroid carcinoma:Current diagnosis and treatment[J].Ann Oncol,2000,11(9):1083-1089.
[7] Fagin JA,Wells SA.Biologic and clinical perspectives on thyroid cancer[J].N Engl J Med,2016,375(23):2307.
[8] Warburg O.On the origin of cancer cells[J].Science,1956,123(3191):309-314.
[9] Deberardinis RJ,Cheng T.Q's next:The diverse functions of glutamine in metabolism,cell biology and cancer[J].Oncogene,2010,29(3):313-324.
[10] Hensley CT,Wasti AT,Deberardinis RJ.Glutamine and cancer:Cell biology,physiology,and clinical opportunities[J].J Clin Invest,2013,123(9):3678-3684.
[11] Son J,Lyssiotis CA,Ying H,et al.Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway[J].Nature,2013,496(7443):101-105.
[12] Lane AN,Fan TW.Regulation of mammalian nucleotide metabolism and biosynthesis[J].Nucleic Acids Res,2015,43(4):2466-2485.
[13] Weinberg F,Hamanaka R,Wheaton WW,et al.Mitochondrial metabolism and ROS generation are essential for Kras-mediated tumorigenicity[J].Proc Natl Acad Sci USA,2010,107(19):8788-8793.
[14] Godwin AK,Meister A,Odwyer PJ,et al.High resistance to cisplatin in human ovarian cancer cell lines is associated with marked increase of glutathione synthesis[J].Proc Natl Acad Sci USA,1992,89(7):3070-3074.
[15] Gao P,Tchernyshyov I,Chang TC,et al.C-myc suppression of miR-23a/b enhances mitochondrial glutaminase expression and glutamine metabolism[J].Nature,2009,458(7239):762-765.
[16] Wise DR,Deberardinis RJ,Mancuso A,et al.Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction[J].Proc Natl Acad Sci USA,2008,105(48):18782-18787.
[17] Hao Y,Samuels Y,Li Q,et al.Oncogenic PIK3CA mutations reprogram glutamine metabolism in colorectal cancer[J].Nat Commun,2016,20(7):11971.
[18] Laplante M,Sabatini DM.MTOR signaling in growth control and disease[J].Cell,2012,149(2):274-93.
[19] Yu Y,Yu X,Fan C,et al.Targeting glutaminase-mediated glutamine dependence in papillary thyroid cancer[J].J Mol Med(Berl),2018,96(8):777-790.
[20] Chen Z,Lin J,Feng S,et al.SIRT4 inhibits the proliferation,migration,and invasion abilities of thyroid cancer cells by inhibiting glutamine metabolism[J].Onco Targets Ther,2019,28(12):2397-2408.
[21] Xiang Y,Stine ZE,Xia J,et al.Targeted inhibition of tumor-specific glutaminase diminishes cell-autonomous tumorigenesis[J].J Clin Invest,2015,125(6):2293-2306.
[22] Gross MI,Demo SD,Dennison JB,et al.Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer[J].Mol Cancer Ther,2014,13(4):890-901.
[23] Jacque N,Ronchetti AM,Larrue C,et al.Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition[J].Blood,2015,126(11):1346-1356.

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备注/Memo

备注/Memo:
基金项目:陕西省榆林市科技计划项目(2019-185-32)
更新日期/Last Update: 2022-09-05