[1]王双梅,常花蕾,冯 燕,等.藏红花提取液对兔慢性高眼压视网膜神经节细胞损伤的保护作用研究[J].陕西医学杂志,2022,51(8):907-910,915.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.001]
　WANG Shuangmei,CHANG Hualei,FENG Yan,et al.Protective effect of crocus sativus extract on retinal ganglion cells injury in rabbits with chronic ocular hypertension[J].,2022,51(8):907-910,915.[doi:DOI:10.3969/j.issn.1000-7377.2022.08.001]
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藏红花提取液对兔慢性高眼压视网膜神经节细胞损伤的保护作用研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年8期
页码:
907-910,915
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Protective effect of crocus sativus extract on retinal ganglion cells injury in rabbits with chronic ocular hypertension
作者:
王双梅; 常花蕾; 冯 燕; 于敬妮
(西安市人民医院 西安市第四医院,陕西 西安710004)
Author(s):
WANG Shuangmei; CHANG Hualei; FENG Yan; YU Jingni
(Xi'an People's Hospital,Xi'an 710004,China)
关键词:
藏红花;  高眼压;  视网膜神经节细胞;  细胞凋亡;  细胞损伤;  兔子
Keywords:
Crocus sativus;  Ocular hypertension;  Retinal ganglion cell;  Apoptosis;  Cell damage;  Rabbit
分类号:
R 773.1
DOI:
DOI:10.3969/j.issn.1000-7377.2022.08.001
文献标志码:
A
摘要:
的:探讨藏红花提取液对兔慢性高眼压视网膜神经节细胞损伤的保护作用。方法:将新西兰白兔随机分为对照组、高眼压组、治疗Ⅰ组和Ⅱ组,每组5只。高眼压组和治疗Ⅰ、Ⅱ组的兔眼前房注入3 g/L复方卡波姆溶液制作慢性高眼压模型,对照组仅作前房穿刺。治疗Ⅰ、Ⅱ组动物每日耳缘分别静注藏红花提取液20、80 mg/kg; 高眼压组和对照组各注射2 ml 0.9%氯化钠溶液,连续28 d。然后所有动物经心脏灌流固定,取球后视神经和视网膜,视神经包埋后作超薄切片,电镜下观察节细胞轴突; 视网膜视神经作冰冻切片,进行HE染色、TUNEL染色及免疫组化检测。结果:高眼压组视网膜各层细胞排列紊乱,节细胞数目减少,神经纤维层明显变薄,轴突数目显著减少; 治疗组明显改善,而治疗Ⅱ组视网膜形态结构接近对照组,各层结构层次清楚,细胞排列整齐,细胞数量基本正常。高眼压组视网膜中的凋亡节细胞及Caspase 3阳性细胞数量最多,注射藏红花提取液后凋亡的节细胞及Caspase 3阳性细胞数目明显减少,在治疗Ⅱ组仅见个别凋亡的节细胞及偶见Caspase 3阳性细胞。结论:慢性高眼压可导致兔眼视网膜神经节细胞及轴突结构损害、视网膜节细胞凋亡; 藏红花提取液可通过减少节细胞凋亡、降低Caspase 3表达,从而保护视网膜神经节细胞。
Abstract:
Objective:To investigate the protective effect of crocus sativus extract on the retinal ganglion cells injury in the rabbits with chronic ocular hypertension(OHT).Methods:Twenty rabbits were randomly divided into control group,OHT group,treatment group Ⅰ and Ⅱ,with 5 rabbits in each group.OHT model was induced by injection of 3 g/L carbomer into anterior chamber in the OHT group and two treatment groups,while anterior chamber paracentesis without injection was performed in the control group.Rabbits in treatment group Ⅰ and Ⅱ were intravenously injected with crocus sativus extract 20 and 80 mg/kg respectively,while rabbits in the OHT and control groups were injected with 2 ml of 0.9% sodium chloride solution for 28 consecutive days.Then all the rabbits were sacrificed by perfusion and optic nerves behind the eyeball and retina slices were collected.The frozen slices were examined by a conventional transmission electron microscopy and the apoptosis of retinal ganglion cell was detected by immuno-histochemistry,HE and TUNEL staining.Results:In the OHT group,the arrangement of cells in each layer of retina was disordered,the number of ganglion cells decreased,the nerve fiber layer became thinner,and the number of axons decreased significantly.The treatment groups were significantly improved,while the retinal morphology of treatment group Ⅱ was close to the control group,with clear levels of each layer structure,neat arrangement of cells and normal number of cells.In the OHT group,the number of apoptotic ganglion cells and Caspase 3 positive cells in retina was the highest,and decreased significantly after injection of crocus sativus extract,and only individual apoptotic ganglion cells and occasionally Caspase 3 positive cells were found.Conclusion:Chronic OHT can lead to damage of retinal ganglion cells and axon structure and apoptosis of retinal ganglion cells in rabbit eyes.Crocus sativus extract can protect retinal ganglion cells by reducing ganglion cell apoptosis and Caspase 3 expression.

参考文献/References:

[1] 吴嘉雯,张圣海.炎症相关机制在青光眼视网膜视神经损伤与保护中的作用[J].中国眼耳鼻喉科杂志,2022,22(2):205-208.
[2] 逯 鹏,王佳森,姜善红,等.藏红花酸预处理对大鼠心肌缺血再灌注损伤的影响及机制探讨[J].山东医药,2017,57(46):41-43.
[3] Hosseini A,Razavi BM,Hosseinzadeh H.Pharmacokinetic properties of saffron and its active components[J].Eur J Drug Metab Pharmacokinet,2018,43(4):383-390.
[4] 张 晨,吕雷锋,李 苗,等,藏红花通过抗凋亡、抗炎和抗水肿机制发挥对大鼠脊髓损伤模型的神经保护作用[J].西安交通大学学报:医学版,2017,38(3):280-289.
[5] 杨敏杰,刘 伟,涂宏飞,等.藏红花素保护溃疡性结肠炎模型大鼠的作用及相关机制[J].中国组织工程研究,2020,24(29):4673-4679.
[6] 张兆旺.中药药剂学[M].北京:中国中医药出版社,2003:122-127.
[7] 任燕如,唐由之.复方卡波姆溶液诱导慢性实验性兔高眼压模型研究现状[J].眼科新进展,2010,30(7):686-689.
[8] 董胜利,仝警安.中西医结合治疗视神经萎缩90例临床研究[J].陕西中医,2018,39(12):1735-1737.
[9] Li XM,Huang D,Yu Q,et al.Neuroligin-3 protects retinal cells from H2O2-induced cell death via activation of Nrf2 signaling[J].BBRC,2018,502(1):166-172.
[10] Wang L,Chen HC,Yang X,et al.The novel chalcone analog L2H17 protects retinal ganglion cells from oxidative stress-induced apoptosis[J].Neural Regen Res,2018,13(9):1665-1672.
[11] Hsueh YJ,Chen YN,Tsao YT,et al.The pathomechanism,antioxidant biomarkers,and treatment of oxidative stress-related eye diseases[J].Int J Mol Sci,2022,23:1-26.
[12] Ao N,Yang J,Du J.Effect and mechanism of liraglutide on the apoptosis of human hepatocellular carcinoma HepG2 cells induced with palmitic acid[J].Zhonghua Gan Zang Bing Za Zhi,2019,27(6):445-449.
[13] Leone S,Recinella L,Chiavaroli A,et al.Phytotherapic use of the Crocus ativus L.(Saffron)and its potential applications:A brief overview[J].Phytother Res,2018,32(12):2364-2375.
[14] 常花蕾,朱 娟,杨新光,等.藏红花素对大鼠视网膜缺血再灌注损伤的保护作用[J].陕西医学杂志,2019,48(7):827-830,845.
[15] Fernández-Albarral JA,Hoz R,Ramírez AI,et al.Beneficial effects of saffron(crocus sativus L)in ocular pathologies,particularly neurodegenerative retinal diseases[J].Neural Regen Res,2020,15(8):1408-1416.
[16] Yang X,Huo F.Crocin inhibits oxidative stress and pro-inflammatory response of microglial cells associated with diabetic retinopathy through the activation of PI3K/Akt signaling pathway[J].Mol Neurosci,2017,61(4):581-589.
[17] 吕伯昌,许治国,陈 涛,等.藏红花素与灯盏细辛对慢性高眼压模型大鼠视神经保护作用的比较[J].中华实验眼科杂志,2016,34(11):990-996.
[18] Moini JA,Karimi A,Nasimi DAR,The potential role of saffron(crocus sativus L)and its components in oxidative stress in diabetes mellitus:A systematic review[J].Clin Nutr Espen,2022,48:148-157.
[19] Lv BC,Chen T,Xu ZG,et al.Crocin protects retinal ganglion cells against H2O2-induced damage through the mitochondrial pathway and activation of NF-κB[J].Mol Med,2016,37(1):225-232.
[20] 齐 赟,张艺馨,程育宏.藏红花素对视网膜缺血再灌注损伤小鼠视网膜神经节细胞的保护作用及其机制研究[J].眼科新进展,2022,42(1):6-10.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(81273902)
更新日期/Last Update: 2022-08-04