[1]贺 轲,李 凯.真核基因组编码大量长链非编码RNA癌易感性候选基因15在肺癌细胞中表达上调且通过miR-153-3p激活Wnt/β-catenin通路调控A549细胞增殖、侵袭及化疗敏感性实验研究[J].陕西医学杂志,2022,51(7):776-782.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.002]
　HE Ke,LI Kai.LncRNA CASC15 was up-regulated in lung cancer cells and activated Wnt/β-catenin pathway through miR-153-3p to regulate the proliferation,invasion and chemosensitivity of A549 cells[J].,2022,51(7):776-782.[doi:DOI:10.3969/j.issn.1000-7377.2022.07.002]
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真核基因组编码大量长链非编码RNA癌易感性候选基因15在肺癌细胞中表达上调且通过miR-153-3p激活Wnt/β-catenin通路调控A549细胞增殖、侵袭及化疗敏感性实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年7期
页码:
776-782
栏目:
基础研究
出版日期:
2022-07-05

文章信息/Info

Title:
LncRNA CASC15 was up-regulated in lung cancer cells and activated Wnt/β-catenin pathway through miR-153-3p to regulate the proliferation,invasion and chemosensitivity of A549 cells
作者:
贺 轲1; 李 凯2
(1.广东省第二人民医院普外一科,广东 广州510317; 2.西安市第三医院心血管外科,陕西 西安710032)
Author(s):
HE Ke; LI Kai
(Department of General Surgery,the Second People's Hospital of Guangdong Province,Guangzhou 510317,China)
关键词:
lncRNA CASC15;  miR-153-3p;  A549细胞;  Wnt/β-catenin;  增殖;  侵袭;  化疗敏感性
Keywords:
LncRNA CASC15;  miR-153-3p;  A549 cells;  Wnt/β-catenin;  Proliferation;  Invasion;  Chemosensitivity
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2022.07.002
文献标志码:
A
摘要:
目的:探讨真核基因组编码大量长链非编码RNA癌易感性候选基因15(lncRNA CASC15)调控肺癌A549细胞增殖、侵袭和化疗敏感性的作用机制。方法:实时荧光定量PCR实验分析lncRNA CASC15、miR-153-3p在65例肺癌组织、50例癌旁组织、肺癌A549细胞和正常肺上皮BEAS-2B细胞中的差异表达。选取A549细胞进行培养,并将siRNA CASC15转染A549细胞,分析下调CASC15对A549细胞增殖和侵袭的影响,用不同浓度顺铂(0、2、4、8、16 μg/ml)处理A549细胞后,检测细胞活力和凋亡率。miRcode预测lncRNA CASC15的潜在结合靶点,双荧光素酶报告基因实验进一步分析与miR-153-3p之间的相关性。下调miR-153-3p或同时上调lncRNA CASC15和miR-153-3p表达,分析A549细胞增殖、侵袭和化疗敏感性情况。过表达或下调miR-153-3p,检测Wnt/β-catenin通路相关蛋白表达。XAV939作用细胞后进一步检测miR-153-3p对A549细胞的增殖、侵袭和化疗敏感性的影响。结果:与癌旁正常组织或BEAS-2B细胞相比,肺癌组织或A549细胞中lncRNA CASC15表达上调(均P<0.01),miR-153-3p表达下调(均P<0.01)。与siRNA NC组相比,CASC15 siRNA组A549细胞活力和侵袭能力明显降低,增强了细胞对顺铂的化疗敏感性; lncRNA CASC15靶向miR-153-3p; miR-153-3p inhibitor转染A549细胞对癌细胞增殖和侵袭能力具有显著促进作用,下调了细胞对顺铂的化疗敏感性; 上调lncRNA CASC15与miR-153-3p表达,显著上调了A549细胞的活力和侵袭能力。下调miR-153-3p激活了A549细胞内Wnt/β-catenin通路,XAV939作用细胞后影响了A549细胞增殖、侵袭及其化疗敏感性。结论:lncRNA CASC15在肺癌A549细胞中表达上调且通过miR-153-3p激活Wnt/β-catenin通路调控A549细胞增殖、侵袭及化疗敏感性。
Abstract:
Objective:To investigate the mechanism of lncRNA CASC15 regulating the proliferation,invasion and chemosensitivity of lung cancer A549 cells.Methods:The differential expressions of lncRNA CASC15 and miR-153-3p in 65 lung cancer tissues,50 paracancerous tissues,lung cancer A549 cells and normal lung epithelial BEAS-2B cells were analyzed by real-time quantitative PCR.A549 cells were selected for culture,and siRNA CASC15 was transfected into A549 cells to analyze the effects of down-regulation of CASC15 on proliferation and invasion of A549 cells.A549 cells were treated with different concentrations of cisplatin(0,2,4,8,16 μg/ml)to detect cell viability and apoptosis rate.The miRcode predicted the potential binding target of lncRNA CASC15,and the correlation with miR-153-3p was further analyzed by dual luciferase reporter gene assay.Down-regulation of miR-153-3p or up-regulation of lncRNA CASC15 and miR-153-3p were performed to analyze the proliferation,invasion and chemosensitivity of A549 cells.MiR-153-3p was overexpressed or down-regulated to detect the expression of Wnt/β-catenin pathway related proteins.The effects of miR-153-3p on the proliferation,invasion and chemosensitivity of A549 cells were further detected after XAV939 treatment.Results:LncRNA CASC15 was up-regulated in lung cancer tissues or A549 cells compared with adjacent normal tissues or BEAS-2B cells,and the expression of miR-153-3p was down-regulated(all P<0.01).Compared with the siRNA NC group,the viability and invasion ability of A549 cells in the CASC15 siRNA group were significantly decreased,which enhanced the sensitivity of cells to cisplatin chemotherapy.LncRNA CASC15 targeted miR-153-3p.MiR-153-3p inhibitor transfection of A549 cells significantly promoted the proliferation and invasion ability of cancer cells,and down-regulated the sensitivity of cells to cisplatin chemotherapy.The up-regulated expression of lncRNA CASC15 and miR-153-3p significantly up-regulated the viability and invasion ability of A549 cells.Down-regulation of miR-153-3p activated the Wnt/β-catenin pathway in A549 cells,and XAV939 affected the proliferation,invasion and chemosensitivity of A549 cells.Conclusion:LncRNA CASC15 was up-regulated in lung cancer A549 cells and activated Wnt/ β-catenin pathway through miR-153-3p to regulate the proliferation,invasion and chemosensitivity of A549 cells.

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备注/Memo

备注/Memo:
基金项目:广东省医学科学技术基金资助项目(C2021007)
更新日期/Last Update: 2022-07-05