[1]霍君艺,赵卓伟,段策中,等.沉默信息调节因子2同系物1在人瘢痕疙瘩组织中的表达及其与氧化应激指标相关性研究[J].陕西医学杂志,2022,51(6):643-646,653.[doi:DOI:10.3969/j.issn.1000-7377.2022.06.001]
　HUO Junyi,ZHAO Zhuowei,DUAN Cezhong,et al.Expression of silent information regulator 2 homologue 1 in human keloid tissue and its correlation with oxidative stress indicators[J].,2022,51(6):643-646,653.[doi:DOI:10.3969/j.issn.1000-7377.2022.06.001]
点击复制

沉默信息调节因子2同系物1在人瘢痕疙瘩组织中的表达及其与氧化应激指标相关性研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年6期
页码:
643-646,653
栏目:
基础研究
出版日期:
2022-06-05

文章信息/Info

Title:
Expression of silent information regulator 2 homologue 1 in human keloid tissue and its correlation with oxidative stress indicators
作者:
霍君艺; 赵卓伟; 段策中; 华 振; 荆银磊; 孟祥海
(空军军医大学唐都医院烧伤整形科,陕西 西安 710038)
Author(s):
HUO Junyi; ZHAO Zhuowei; DUAN Cezhong; HUA Zhen; JING Yinlei; MENG Xianghai
(Department of Burn and Plastic Surgery,Tangdu Hospital of Air Force Medical University,Xi'an 710038,China)
关键词:
沉默信息调节因子2同系物1;  瘢痕疙瘩;  氧化应激;  病程;  表达水平;  相关性
Keywords:
Silent information regulator 2 homologue 1;  Keloid;  Oxidative stress;  Disease course;  Expression level;  Correlation
分类号:
R 619.6
DOI:
DOI:10.3969/j.issn.1000-7377.2022.06.001
文献标志码:
A
摘要:
目的:研究沉默信息调节因子2同系物1(SIRT1)在人瘢痕疙瘩组织中的表达情况,并探讨其表达与氧化应激指标的相关性。方法:选取瘢痕疙瘩患者82例,根据病程不同分为A组(0～6个月)、B组(6～12个月)和C组(>12个月)。将瘢痕组织分为浸润部、增生部和老化部三部分。在正常组织和瘢痕疙瘩组织,采用实时荧光定量PCR(qPCR)法检测SIRT1 mRNA表达,采用免疫印迹法检测SIRT1、p65和p-p65蛋白表达,并检测氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)和晚期氧化蛋白产物(AOPP)]含量。结果:在各组内,SIRT1 mRNA和蛋白在瘢痕疙瘩组织中的表达水平显著低于正常组织(均P<0.05)。三组间SIRT1 mRNA和蛋白在正常组织和瘢痕疙瘩组织中的表达水平比较差异无统计学意义(均P>0.05)。在各组内,瘢痕疙瘩组织老化部、增生部SIRT1 mRNA和蛋白表达水平显著高于浸润部,且老化部高于增生部(均P<0.05)。三组间瘢痕疙瘩组织不同部位SIRT1 mRNA和蛋白表达水平比较差异无统计学意义(均P>0.05)。正常组织和瘢痕疙瘩组织p65蛋白表达水平比较差异无统计学意义(P>0.05)。瘢痕疙瘩组织p-p65蛋白表达水平和p-p65/p65,以及SOD、MDA和AOPP含量显著高于正常组织(均P<0.05)。在82例瘢痕疙瘩组织中,SIRT1 mRNA和蛋白表达水平与SOD、MDA和AOPP呈负相关(均P<0.05)。结论:SIRT1在人瘢痕疙瘩组织中表达降低,其表达水平与人瘢痕疙瘩病程无关,而与其在瘢痕疙瘩组织中的表达部位有关,并且与瘢痕疙瘩组织中氧化应激呈负相关。
Abstract:
Objective:To study the expression of silent information regulator 2 homologue 1(SIRT1)in human keloid tissue,and to explore the correlation between its expression and oxidative stress indicators.Methods: A total of 82 patients with keloids were selected and divided into group A(0-6 months),group B(6-12 months)and group C(>12 months)according to the course of the disease,and divided the scar tissue into three parts:infiltration part,hyperplasia part and aging part.In normal tissue and keloid tissue,real-time quantitative PCR(qPCR)was used to detect the expression of SIRT1 mRNA,and Western blot was used to detect the expression of SIRT1,p65 and p-p65 proteins,and the contents of oxidative stress indicators(SOD,MDA and AOPP)were detected.Results:In each group,the expression levels of SIRT1 mRNA and protein in human keloid tissues were significantly lower than those in normal tissues(all P<0.05).There was no significant difference in the expression of SIRT1 mRNA and protein in normal tissues and keloid tissues among the three groups(all P>0.05).In each group,the expression levels of SIRT1 mRNA and protein in aging parts and hyperplasia parts of keloid tissues were significantly higher than those in infiltration parts,and the expression levels of SIRT1 mRNA and protein in aging parts were higher than those in hyperplasia parts(all P<0.05).There was no significant difference in SIRT1 mRNA and protein expression in different parts among the three groups(all P>0.05).There was no significant difference in p65 protein expression between normal tissues and keloid tissues(P>0.05).The level of p-p65 protein expression,p-p65/p65,as well as the levels of SOD,MDA and AOPP in keloid tissues were significantly higher than those in normal tissues(all P<0.05).In 82 keloid tissues,SIRT1 mRNA and protein expression levels were negatively correlated with SOD,MDA and AOPP(all P<0.05).Conclusion:The expression level of SIRT1 in human keloid tissue is decreased,and its expression level is not related to the course of human keloid,but related to its expression site in keloid tissue,and negatively related to oxidative stress in keloid tissue.

参考文献/References:

[1] 朱秀梅,黄亚川,范荣辉.氟尿嘧啶配伍曲安奈德治疗瘢痕疙瘩46例[J].陕西医学杂志,2016,45(9):1241-1242.
[2] 崔 晶,金哲虎,金 珊,等.多配体蛋白聚糖-1对瘢痕疙瘩成纤维细胞增殖及迁移的影响[J].中国皮肤性病学杂志,2020,34(6):617-621.
[3] Ogawa R,Dohi T,Tosa M,et al.The latest strategy for keloid and hypertrophic scar prevention and treatment:The Nippon Medical School(NMS)protocol[J].J Nippon Med Sch,2021,88(1):2-9.
[4] 张慧娟,王 强,姚 放.异甘草素对瘢痕疙瘩成纤维细胞胶原合成的影响及机制[J].实用皮肤病学杂志,2021,14(5):261-265.
[5] 岳宗进,刘汝银,于 露,等.补骨脂素调控SKP2/PTHrP/SIRT1轴缓解炎症环境下髓核细胞退变的作用[J].解放军医学杂志,2021,46(7):657-665.
[6] 杨 洛,唐凤娟,张 祥,等.松果菊苷通过调控SIRT1/PGC-1α信号通路改善肥胖糖尿病小鼠肝损伤[J].医学研究杂志,2020,49(3):85-89,60.
[7] Liu T,Ma X,Ouyang T,et al.Efficacy of 5-aminolevulinic acid-based photodynamic therapy against keloid compromised by downregulation of SIRT1-SIRT3-SOD2-mROS dependent autophagy pathway [J].Redox Biol,2019,20:195-203.
[8] Bai XZ,Liu JQ,Yang LL,et al.Identification of sirtuin 1 as a promising therapeutic target for hypertrophic scars[J].Br J Pharmacol,2016,173(10):1589-1601.
[9] 戴晓刚,李小莹,余厚友.自体脂肪移植联合脉冲染料激光治疗烧伤后增生性瘢痕效果及对患者瘢痕血流灌注的影响[J].陕西医学杂志,2021,50(11):1404-1407.
[10] 吕开阳,夏照帆.中日两国瘢痕及瘢痕疙瘩的诊治异同与思考[J].中华烧伤杂志,2021,37(4):306-311.
[11] Zhang Y,Anoopkumar-Dukie S,Arora D,et al.Review of the anti-inflammatory effect of SIRT1 and SIRT2 modulators on neurodegenerative diseases[J].Eur J Pharmacol,2020,867:172847.
[12] 曹 佳,杨华英,何 艺,等.姜黄素通过激活SIRT1调节脓毒症小鼠模型的认知功能障碍研究[J].陕西中医,2020,41(3):301-307.
[13] Palmieri B,Vadalà M,Laurino C.Nutrition in wound healing:Investigation of the molecular mechanisms,a narrative review[J].J Wound Care,2019,28(10):683-693.
[14] Rodrigues M,Kosaric N,Bonham CA,et al.Wound healing:A cellular perspective[J].Physiol Rev,2019,99(1):665-706.
[15] Li K,Zhai M,Jiang L,et al.Tetrahydrocurcumin ameliorates diabetic cardiomyopathy by attenuating high glucose-induced oxidative stress and fibrosis via activating the SIRT1 pathway[J].Oxid Med Cell Longev,2019,2019:6746907.
[16] Song L,Chen TY,Zhao XJ,et al.Pterostilbene prevents hepatocyte epithelial-mesenchymal transition in fructose-induced liver fibrosis through suppressing miR-34a/Sirt1/p53 and TGF-β1/Smads signalling[J].Br J Pharmacol,2019,176(11):1619-1634.
[17] Chen L,Wang Y,Li S,et al.Exosomes derived from GDNF-modified human adipose mesenchymal stem cells ameliorate peritubular capillary loss in tubulointerstitial fibrosis by activating the SIRT1/eNOS signaling pathway[J].Theranostics,2020,10(20):9425-9442.
[18] Jiang L,Deng Y,Li W,et al.Arctigenin suppresses fibroblast activity and extracellular matrix deposition in hypertrophic scarring by reducing inflammation and oxidative stress[J].Mol Med Rep,2020,22(6):4783-4791.
[19] 李仕一,杨锦秀,陈敏亮.氧化应激与病理性瘢痕研究进展[J].中华医学美学美容杂志,2020,26(5):454-456.
[20] Singh V,Ubaid S.Role of silent information regulator 1(SIRT1)in regulating oxidative stress and inflammation[J].Inflammation,2021,44(5):2142.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81302377)
更新日期/Last Update: 2022-06-06