[1]施 健,胡千慧,张雪杰.口腔鳞癌组织长链非编码RNA LINC01605表达与临床病理特征关系研究[J].陕西医学杂志,2022,51(5):617-620.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.024]
　SHI Jian,HU Qianhui,ZHANG Xuejie.Relationship between expression of long-chain noncoding RNA LINC01605and clinicopathological features in oral squamous cell carcinoma[J].,2022,51(5):617-620.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.024]
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口腔鳞癌组织长链非编码RNA LINC01605表达与临床病理特征关系研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年5期
页码:
617-620
栏目:
临床病理
出版日期:
2022-05-05

文章信息/Info

Title:
Relationship between expression of long-chain noncoding RNA LINC01605and clinicopathological features in oral squamous cell carcinoma
作者:
施 健1; 胡千慧1 ; 张雪杰2
(1.锦州医科大学附属第二医院,辽宁 锦州121000; 2.锦州医科大学附属第一医院神经内科,辽宁 锦州121012)
Author(s):
SHI Jian; HU Qianhui; ZHANG Xuejie
(Second Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
关键词:
鳞状细胞癌;  口腔;  长链非编码RNA LINC01605;  增殖;  预后;  相关性
Keywords:
Squamous cell carcinoma;  Oral;  LncRNA LINC01605;  Proliferation;  Prognosis;  Relevance
分类号:
R 739.8
DOI:
DOI:10.3969/j.issn.1000-7377.2022.05.024
文献标志码:
A
摘要:
目的:检测长链非编码RNA(LncRNA)LINC01605在口腔鳞癌组织中的表达特征,观察其表达的临床意义。方法:收集确诊为口腔鳞癌并行手术治疗的患者共58例,留取术后肿瘤组织和距肿物边缘>3 cm的正常口腔黏膜组织,应用实时荧光定量PCR法检测LINC01605的表达,应用免疫组化法检测Ki67的表达。结果:实时荧光定量PCR结果显示LINC01605在口腔鳞癌中的表达明显高于正常口腔黏膜组织(t=4.31,P=0.010),LINC01605在不同肿瘤最大径、分化程度及有无脉管癌栓分组的表达中差异有统计学意义(均P<0.05)。LINC01605的表达与Ki67增殖指数呈正相关性(r=0.64,P=0.029),LINC01605的表达与术后生存时间相关。结论:口腔鳞癌中LINC01605的表达升高,在病变形成和进展中有重要促进作用,LINC01605与增殖指数相关,术后检测LINC01605的表达对判断患者预后可能有一定价值。
Abstract:
Objective:To detect the expression characteristics of long non-coding RNA(LncRNA)LINC01605 in oral squamous cell carcinoma,and observe its clinical significance.Methods: A total of 58 patients with oral squamous cell carcinoma were collected.The tumor tissues and normal oral mucosa tissues(>3 cm from the edge of the tumor)were collected.The expression of LINC01605 was detected by real-time fluorescence quantitative PCR,and the expression of Ki67 was detected by immunohistochemistry.Results:Real-time fluorescence quantitative PCR results showed that the expression of LINC01605 in oral squamous cell carcinoma was significantly higher than that in normal oral mucosa(t=4.31,P=0.010).There were significant differences in the expression of LINC01605 in different tumor diameter,differentiation degree and groups with or without vascular tumor thrombus(all P<0.05).The expression of LINC01605 was positively correlated with Ki67 proliferation index(r=0.64,P=0.029),and the expression of LINC01605 was correlated with postoperative survival time.Conclusion:The expression of LINC01605 in oral squamous cell carcinoma is increased,which plays an important role in promoting the formation and progression of the disease.LINC01605 is correlated with proliferation index.Detection of LINC01605 expression after operation may have certain value in judging the prognosis of patients.

参考文献/References:

[1] 白 雪,张 斌,汪 潇,等.miR-637调控RING1表达抑制口腔鳞癌Tac-8113细胞增殖实验研究[J].陕西医学杂志,2020,49(8):923-927.
[2] 王 楠,席 锦.口腔癌发病危险因素分析[J].陕西医学杂志,2021,50(4):417-420.
[3] Yao Y,Chen S,Lu N,et al.LncRNA JPX overexpressed in oral squamous cell carcinoma drives malignancy via miR-944/CDH2 axis[J].Oral Dis,2021,27(4):924-933.
[4] He K,Zhu ZB,Shu R,et al.LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway[J].World J Surg Oncol,2020,18(1):261-262.
[5] Yang Y,Chen D,Liu H,et al.Increased expression of LncRNA CASC9 promotes tumor progression by suppressing autophagy-mediated cell apoptosis via the AKT/mTOR pathway in oral squamous cell carcinoma[J].Yang K Cell Death Dis,2019,10(2):41-42.
[6] Ding L,Ren J,Zhang D,et al.A novel stromal LncRNA signature reprograms fibroblasts to promote the growth of oral squamous cell carcinoma via LncRNA-CAF/interleukin-33[J].Carcinogenesis,2018,39(3):397-406.
[7] 李冠睿,李建武,颜家渝.长链非编码RNA LINC00478抑制微小RNA-214对口腔鳞癌细胞顺铂耐药性的影响[J].暨南大学学报:自然科学与医学版,2021,42(1):19-25.
[8] 蒋英英,秦 星,张建军,等.长链非编码RNA COL11A1-208在口腔鳞状细胞癌中的表达及临床意义[J].上海交通大学学报:医学版,2020,40(10):1334-1339.
[9] Wang XY,Wang L,Xu PC,et al.LINC01605 promotes the proliferation of laryngeal squamous cell carcinoma through targeting miR-493-3p[J].Eur Rev Med Pharmacol Sci,2019,23(23):10379-10386.
[10] Qin Z,Wang Y,Tang J,et al.High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer[J].Biosci Rep,2018,38(5):20180562.
[11] Hu SS,Fu L,Han SY,et al.LINC01605 regulates proliferation,migration and invasion of colorectal cancer cells via targeting miR-3960/SOX11[J].Eur Rev Med Pharmacol Sci,2021,25(3): 1322-1329.
[12] Luo Y,Ye J,Wei J,et al.Long noncoding RNAbased risk scoring system predicts prognosis of alcoholrelated hepatocellular carcinoma[J].Mol Med Rep,2020,22(2):997-1007.
[13] 高玉霞,董学彩,王文翔,等.长链非编码RNA HOTTIP在子宫内膜癌患者组织中的表达及其临床意义[J].中国肿瘤生物治疗杂志,2020,27(12):1378-1382.
[14] 陆 伟,王永武,淦 岷.LncRNA CCAT2/miR-145/p70S6K1分子轴调控口腔鳞癌细胞侵袭转移[J].中国现代医生,2020,58(30):53-57.
[15] 徐 怡,董伟杰.长链非编码RNA H1通过调控EZH2对口腔鳞癌细胞增殖和侵袭的影响[J].重庆医学,2020,49(20):3342-3346.
[16] Zhang X,Guo B,Zhu Y,et al.Up-regulation of plasma LncRNA CACS15 distinguished early-stage oral squamous cell carcinoma patient[J].Oral Dis,2020,26(8):1619-1624.
[17] Gao L,Wang S,Meng J,et al.LncRNA LUADT1 promotes oral squamous cell carcinoma cell proliferation by regulating miR-34a/GAS1 axis[J].Cancer Manag Res,2020,13(12):3401-3407.
[18] Yue M,Liu T,Yan G,et al.LINC01605,regulated by the EP300-SMYD2 complex,potentiates the binding between METTL3 and SPTBN2 in colorectal cancer[J].Cancer Cell Int,2021,21(1):504.
[19] Garcia-Etxebarria K,Clos-Garcia M,Telleria O,et al.Interplay between genome,metabolome and microbiome in colorectal cancer[J].Cancers(Basel),2021,13(24):6216.
[20] 王娜娜,杨 川.喉鳞状细胞癌的转录组数据分析及生物标志物筛选[J].癌症进展,2021,19(7):671-678.
[21] 邢益桓,付 斌,夏 鹰.LncRNA XIST介导的ceRNA调控网络在恶性肿瘤中作用的研究进展[J].中国肿瘤生物治疗杂志,2020,27(9):1062-1067.
[22] Wu X,Ma J,Chen J,et al.LncRNA CACS15 regulates tongue squamous cell carcinoma cell behaviors and predicts survival[J].BMC Oral Health,2019,19(1):231-233.
[23] Chen ZF,Wang Y,Sun LL,et al.LncRNA SNHG20 enhances the progression of oral squamous cell carcinoma by regulating the miR-29a/DIXDC1/Wnt regulatory axis[J].Eur Rev Med Pharmacol Sci,2020,24(10):5436-5445.

更新日期/Last Update: 2022-05-05