[1]赵 瑾,胡海燕,周艳红.高迁移率族蛋白B1过表达对子宫内膜癌细胞增殖、周期和凋亡的影响及机制研究[J].陕西医学杂志,2022,51(5):539-542.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.006]
　ZHAO Jin,HU Haiyan,ZHOU Yanhong.Effect and mechanism of HMGB1 overexpression on proliferation,cycle and apoptosis of endometrial cancer cells[J].,2022,51(5):539-542.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.006]
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高迁移率族蛋白B1过表达对子宫内膜癌细胞增殖、周期和凋亡的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年5期
页码:
539-542
栏目:
基础研究
出版日期:
2022-05-05

文章信息/Info

Title:
Effect and mechanism of HMGB1 overexpression on proliferation,cycle and apoptosis of endometrial cancer cells
作者:
赵 瑾; 胡海燕; 周艳红
(西安医学院第二附属医院,陕西 西安 710038)
Author(s):
ZHAO Jin; HU Haiyan; ZHOU Yanhong
(Second Affiliated Hospital of Xi'an Medical College,Xi'an 710038,China)
关键词:
高迁移率族蛋白B1;  子宫内膜癌;  细胞增殖;  细胞凋亡;  细胞周期;  B细胞特异性小鼠白血病病毒插入位点1
Keywords:
High mobility group protein B1;  Endometrial cancer;  Cell proliferation;  Apoptosis;  Cell cycle;  B-cell-specific moloney murine leukemia virus insertion site 1
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7377.2022.05.006
文献标志码:
A
摘要:
目的:探讨高迁移率族蛋白(HMG)B1过表达对子宫内膜癌细胞增殖、周期和凋亡的影响及机制。方法:将对数生长期的Ishikawa细胞分为三组:pcDNA3.0组、pcDNA3.0-HMGB1组与对照组。pcDNA3.0组、pcDNA3.0-HMGB1组分别转染pcDNA3.0载体与pcDNA3.0-HMGB1载体,对照组以等体积的磷酸盐缓冲液进行转染。采用MTT法检测细胞增殖指数、Transwell小室检测细胞侵袭与转移指数、流式细胞仪检测细胞凋亡与细胞周期、Western blot检测蛋白表达。结果:转染后36、72 h,pcDNA3.0-HMGB1组的细胞增殖指数高于pcDNA3.0组与对照组(均P<0.05),细胞凋亡指数低于pcDNA3.0组与对照组(均P<0.05)。转染后36、72 h,pcDNA3.0-HMGB1组的G0/G1期比例高于pcDNA3.0组与对照组(均P<0.05),S期和G2/M期比例低于pcDNA3.0组与对照组(均P<0.05)。转染后36、72 h,pcDNA3.0-HMGB1组的细胞侵袭与转移指数高于pcDNA3.0组与对照组(均P<0.05)。转染后36、72 h,pcDNA3.0-HMGB1组的B细胞特异性小鼠白血病病毒插入位点1(Bmi-1)、HMGB1蛋白相对表达水平高于pcDNA3.0组与对照组(均P<0.05)。结论:HMGB1过表达能促进子宫内膜癌细胞增殖、侵袭、转移及Bmi-1蛋白表达,抑制细胞凋亡,调节细胞周期。
Abstract:
Objective:To investigate the effect and mechanism of high mobility group protein(HMG)B1 overexpression on the proliferation,cycle and apoptosis of endometrial cancer cells.Methods: Ishikawa cells in logarithmic growth phase were divided into pcDNA3.0 group,pcDNA3.0-HMGB1 group and control group.The pcDNA3.0 group and pcDNA3.0-HMGB1 group were transfected with pcDNA3.0 vector and pcDNA3.0-HMGB1 vector,respectively.The control group was transfected with equal volume of phosphate buffer.MTT method was used to detect cell proliferation index.Flow cytometry was used to detect cell apoptosis and cell cycle.Transwell chamber was used to detect cell metastasis and invasion.Results:At 36 and 72 hours after transfection,the cell proliferation index of the pcDNA3.0-HMGB1 group was higher than that of pcDNA3.0 group and control group,and the apoptosis index was lower than that of pcDNA3.0 group and control group(all P<0.05); the ratio of G0/G1 phase in pcDNA3.0-HMGB1 group was higher than that of pcDNA3.0 group and control group,while the ratio of S phase and G2/M phase was lower than that of pcDNA3.0 group and control Group(all P<0.05); the cell metastasis and invasion index of pcDNA3.0-HMGB1 group was higher than that of pcDNA3.0 group and control group(all P<0.05); the relative expression levels of B-cell-specific moloney murine leukemia virus insertion site 1(Bmi-1)and HMGB1 proteins in pcDNA3.0-HMGB1 group were higher than those of pcDNA3.0 group and control group(all P<0.05).Conclusion:Overexpression of HMGB1 can promote the proliferation,invasion and metastasis of endometrial cancer cells and the expression of Bmi-1 protein,inhibit apoptosis and regulate cell cycle.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2019SF-051)
更新日期/Last Update: 2022-05-05