[1]段小霞,郭 华,付 婷,等.震颤同系物-5过表达对卵巢癌细胞A2780增殖和凋亡的影响[J].陕西医学杂志,2022,51(5):529-533.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.004]
　DUAN Xiaoxia,GUO Hua,FU Ting,et al.Effect of quaking homolog-5 overexpression on proliferation and apoptosis of ovarian cancer cell line A2780[J].,2022,51(5):529-533.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.004]
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震颤同系物-5过表达对卵巢癌细胞A2780增殖和凋亡的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年5期
页码:
529-533
栏目:
基础研究
出版日期:
2022-05-05

文章信息/Info

Title:
Effect of quaking homolog-5 overexpression on proliferation and apoptosis of ovarian cancer cell line A2780
作者:
段小霞1; 郭 华2; 付 婷1; 晋雅凌1; 周 卓1
(1.陕西省第四人民医院妇产科,陕西 西安710043; 2.西安市中心医院呼吸与危重症医学科,陕西 西安710004)
Author(s):
DUAN Xiaoxia; GUO Hua; FU Ting; JIN Yaling; ZHOU Zhuo
(Department of Obstetrics and Gynecology,the Fourth People's Hospital of Shaanxi,Xi'an 710043,China)
关键词:
卵巢癌;  RNA结合蛋白;  震颤同系物-5;  增殖;  凋亡;  慢病毒
Keywords:
Ovarian cancer;  RNA binding protein;  Quaking homologue-5;  Proliferation;  Apoptosis;  Lentivirus
分类号:
R 737.31
DOI:
DOI:10.3969/j.issn.1000-7377.2022.05.004
文献标志码:
A
摘要:
目的:探讨RNA结合蛋白震颤同系物-5(QKI-5)在卵巢癌细胞中的表达以及对卵巢癌细胞增殖和凋亡的影响。方法:通过免疫印迹实验(Western blot)检测正常卵巢上皮细胞和卵巢癌细胞中QKI-5的表达差异,并选取QKI-5相对表达水平最低的卵巢癌细胞进行过表达慢病毒感染,构建稳定转染细胞系后通过细胞增殖和毒性分析(CCK8)和平板克隆实验检测细胞增殖和克隆能力的变化,通过流式细胞仪检测过表达QKI-5对卵巢癌细胞凋亡的影响。结果:Western blot实验结果显示,在卵巢癌细胞中QKI-5的表达水平相比正常卵巢上皮细胞显著降低,三株卵巢癌细胞中A2780细胞的QKI-5表达水平相对最低,通过慢病毒感染成功构建了稳定过表达QKI-5的A2780细胞系。CCK8和平板克隆实验结果显示,过表达QKI-5基因后A2780细胞增殖活力和克隆形成能力受到抑制(P<0.05)。流式细胞仪检测细胞凋亡率结果显示,QKI-5表达水平的增加能够显著促进A2780细胞的凋亡(P<0.05)。结论:RNA结合蛋白QKI-5在卵巢细胞中表达水平异常降低,QKI-5表达特异性扩增后能够抑制卵巢癌细胞A2780增殖和克隆形成能力并促进癌细胞发生凋亡。
Abstract:
Objective:To investigate the expression of RNA binding protein quaking homologue-5(QKI-5)in ovarian cancer cells and its effect on proliferation and apoptosis of ovarian cancer cells.Methods:The expression of QKI-5 in normal ovarian epithelial cells and ovarian cancer cells was detected by Western blot,and the ovarian cancer cells with the lowest relative expression level of QKI-5 were selected for lentivirus infection.After stable transfected cell lines were constructed,cell proliferation and cloning ability were detected by CCK8 and plate cloning experiments.The effect of QKI-5 overexpression on apoptosis of ovarian cancer cells was detected by flow cytometry.Results:Western blot showed that the expression level of QKI-5 in ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells.The expression level of QKI-5 in A2780 cells was the lowest among the three ovarian cancer cells.The A2780 cell line with stable overexpression of QKI-5 was successfully constructed by lentivirus infection.CCK8 and plate cloning experiments showed that the proliferation activity and clone formation ability of A2780 cells were inhibited after QKI-5 gene overexpression(all P<0.05).The apoptosis rate of A2780 cells was detected by flow cytometry,and the increase of QKI-5 expression level significantly promoted the apoptosis of A2780 cells(P<0.05).Conclusion:The expression level of RNA binding protein QKI-5 is abnormally decreased in ovarian cells.The specific amplification of QKI-5 expression can inhibit the proliferation and clone formation,and promote apoptosis of ovarian cancer cell A2780.

参考文献/References:

[1] Zuberi M,Dholariya S,Khan I,et al.Epigenetic silencing of DAPK1and p16(INK4a)genes by CpG island hypermethylation in epithelial ovarian cancer patients[J].Indian J Clin Biochem,2021,36(2):200-207.
[2] Banerjee S,Tovey H,Bowen R,et al.Abiraterone in patients with recurrent epithelial ovarian cancer:Rincipal results of the phase-Ⅱ cancer of the ovary abiraterone(CORAL)trial(CRUK-A16037)[J].Ther Adv Med Oncol,2020,12(2):1758835920975352.
[3] Herzog TJ.Highlights in ovarian cancer from the European Society for Medical Oncology Virtual Congress 2020:Commentary[J].Clin Adv Hematol Oncol,2020,18(11):19-23.
[4] Feng W,Dean DC,Hornicek FJ,et al.ATR and p-ATR are emerging prognostic biomarkers and DNA damage response targets in ovarian cancer[J].Ther Adv Med Oncol,2020,12(8):1758835920982853.
[5] 苏 游,刘新月,刘巧梅.自噬基因Beclin1在卵巢癌SKOV3细胞中对PI3K/AKT信号通路的影响及其与紫杉醇耐药关系实验研究[J].陕西医学杂志,2021,50(2):143-147.
[6] 张 燕,史文雯.血清CA125、HE4及MMP-10联合检测在卵巢癌早期诊断中的临床价值研究[J].陕西医学杂志,2018,47(8):1072-1074.
[7] Li Y,Zhang X,Gao Y,et al.Development of a genomic signatures-based predictor of initial platinum-resistance in advanced high-grade serous ovarian cancer patients[J].Frontiers in Oncology,2020,10:625866.
[8] Liu T,Cai J,Cai J,et al.EZH2-miRNA positive feedback promotes tumor growth in ovarian cancer[J].Frontiers in Oncology,2020,10:608393.
[9] Shen Y,Li C,Zhou L,et al.G protein-coupled oestrogen receptor promotes cell growth of non-small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling[J].J Cell Mol Med,2021,25(1):284-296.
[10] Hu C,Fang K,Zhang X,et al.Dyregulation of the lncRNA TPT1-AS1 positively regulates QKI expression and predicts a poor prognosis for patients with breast cancer[J].Pathol Res Pract,2020,216(11):153216.
[11] Lu H,Ye Z,Zhai Y,et al.QKI regulates adipose tissue metabolism by acting as a brake on thermogenesis and promoting obesity[J].EMBO Rep,2020,21(1):47929.
[12] Wang JZ,Fu X,Fang Z,et al.QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer[J].J Mol Cell Biol,2021,13(5):347-360.
[13] Zhao Y,Zhang G,Wei M,et al.The tumor suppressing effects of QKI-5 in prostate cancer:A novel diagnostic and prognostic protein[J].Cancer Biology & Therapy,2014,15(1):108-118.
[14] 朱士杰,施正祥.RNA结合蛋白QKI-5、Ezrin在浆液性卵巢癌中的研究[J].中国妇幼健康研究,2018,29(6):713-716.
[15] Xing L,Tian S,Mi W,et al.PRSS1 upregulation predicts platinum resistance in ovarian cancer patients[J].Front Cell Dev Biol,2020,8:618341.
[16] Kim K,Han Y,Jeong S,et al.Prediction of postoperative length of hospital stay based on differences in nursing narratives in elderly patients with epithelial ovarian cancer[J].Methods Inf Med,2019,58(6):222-228.
[17] Shin S,Kim J,Kim D,et al.The Importance of education regarding the effects of anticancer treatment on fertility and ovarian function in the female patients with breast cancer[J].J Menopausal Med,2019,25(3):142-148.
[18] Cao Y,Chen H,Huang Y,et al.Real-world clinical outcomes of olaparib therapy in Chinese patients with advanced serous ovarian cancer treated in Macau[J].Cancer Rep(Hoboken),2019,2(5):1180.
[19] 李 斌,黄 蓉,李红霞,等.党黄抗癌汤配合肿瘤细胞减灭术中腹腔热灌注及化疗治疗Ⅳ期上皮性卵巢癌临床研究[J].陕西中医,2019,40(11):1562-1565,1569.
[20] Himoto Y,Veeraraghavan H,Zheng J,et al.Computed tomography-derived radiomic metrics can identify responders to immunotherapy in ovarian cancer[J].JCO Precis Oncol,2019,15(3):1-13.
[21] 霍 亮,葛倩倩.参葵汤对卵巢癌模型大鼠淋巴细胞、免疫球蛋白及相关炎症因子的影响[J].陕西中医,2018,39(8):995-997.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2021SF-255); 西安市科技计划项目(2019114613YX001SF034)
更新日期/Last Update: 2022-05-05