[1]秦少博,黄增刚.缬沙坦/氨氯地平通过调控miR-21对心肌缺血/再灌注损伤大鼠的保护作用及机制研究[J].陕西医学杂志,2022,51(4):396-400,405.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.003]
　QIN Shaobo,HUANG Zenggang.Protective effect and mechanism of valsartan/amlodipine on myocardial ischemia-reperfusion injury rats by regulating miR-21[J].,2022,51(4):396-400,405.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.003]
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缬沙坦/氨氯地平通过调控miR-21对心肌缺血/再灌注损伤大鼠的保护作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年4期
页码:
396-400,405
栏目:
基础研究
出版日期:
2022-04-05

文章信息/Info

Title:
Protective effect and mechanism of valsartan/amlodipine on myocardial ischemia-reperfusion injury rats by regulating miR-21
作者:
秦少博1; 黄增刚2
(1.西安市第一医院心血管内科,陕西 西安 710002; 2.安康市中医医院制剂中心,陕西 安康 725000)
Author(s):
QIN Shaobo; HUANG Zenggang
(Department of Cardiovascular Medicine,Xi'an No.1 Hospital,Xi'an 710002,China)
关键词:
缬沙坦/氨氯地平;  心肌缺血/再灌注损伤;  miR-21;  保护作用;  动物实验
Keywords:
Valsartan/amlodipine;  Myocardial ischemia-reperfusion injury;  miR-21;  Protective effect;  Animal experiment
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2022.04.003
文献标志码:
A
摘要:
目的:研究缬沙坦/氨氯地平对心肌缺血/再灌注损伤(MIRI)大鼠的保护作用及相关机制。方法:30只大鼠随机分为假手术组、模型组和治疗组,每组10只,模型组和治疗组均建立MIRI模型,给予治疗组30 mg/kg的缬沙坦/氨氯地平药物混悬液8周治疗。HE染色观察各组大鼠心肌组织形态学; 计算梗死心肌占总心肌的比例; MTT法检测各组心肌细胞生存率; rt-PCR法检测各组心肌组织中miR-21和抗过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、核呼吸因子1(NRF1)、线粒体转录因子A(TFAM)mRNA的相对表达。结果:模型组心肌梗死面积比例显著高于假手术组(P<0.05); 治疗组心肌梗死面积比例显著低于模型组(P<0.05)。模型组心肌细胞生存率显著低于假手术组(P<0.05); 治疗组心肌细胞生存率显著高于模型组(P<0.05)。模型组心肌组织中miR-21的相对表达显著高于假手术组(P<0.05); 治疗组心肌组织中miR-21的相对表达显著低于模型组(P<0.05)。模型组心肌组织中PGC-1α、NRF1和TFAM mRNA的相对表达显著低于假手术组(均P<0.05); 治疗组心肌组织中PGC-1α、NRF1和TFAM mRNA的相对表达显著高于模型组(均P<0.05)。结论:缬沙坦/氨氯地平对MIRI 模型大鼠的心肌组织具有良好的保护作用,其机制可能与下调miR-21的表达而上调其下游基因基因PGC-1α、NRF1和TFAM的表达有关。
Abstract:
Objective:To investigate the protective effect and mechanism of valsartan/amlodipine on myocardial ischemia-reperfusion injury(MIRI)rats by regulating miR-21.Methods:30 rats were randomly divided into sham operation group,model group and treatment group,with 10 rats in each group.The model group and treatment group were established of MIRI model,then the treatment group was treated with 30 mg/kg valsartan/amlodipine suspension for 8 weeks.The myocardial morphology of each group was observed by HE staining.The proportion of myocardial infarction was calculated.The myocardial cell survival rate was detected by MTT assay.The expression levels of miR-21,peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α),nuclear respiratory factor 1(NRF1),mitochondrial transcription factor A(TFAM)mRNAs were detected by rt-PCR.Results:The proportion of myocardial infarction of model group was higher than that of sham operation group(P<0.05),while the treatment group was lower than model group(P<0.05).The myocardial cell survival rate of model group was lower than that of sham operation group(P<0.05),while the treatment group was higher than model group(P<0.05).The expression of miR-21 of model group was higher than that of sham operation group(P<0.05),while the treatment group was lower than model group(P<0.05).The expression levels of PGC-1α,NRF1 and TFAM mRNAs in model group were lower than those in sham operation group(all P<0.05),while the treatment group was higher than model group(P<0.05).Conclusion:Valsartan/amlodipine has a good protective effect on myocardial tissue of MIRI rats,and its mechanism may be related to down-regulation of miR-21 expression and up-regulation of the downstream genes PGC-1α,NRF1 and TFAM.

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备注/Memo

备注/Memo:
基金项目:陕西省社会发展科技攻关项目(2016SF-339)
更新日期/Last Update: 2022-04-07