[1]樊 力,冯 亮.去细胞异种神经支架对大鼠足底创面触觉功能重建的影响及机制研究[J].陕西医学杂志,2022,51(4):387-390,395.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.001]
　FAN Li,FENG Liang.Effect and mechanism of acellular xenogeneic nerve scaffold on reconstruction of tactile function of rat plantar wound surface[J].,2022,51(4):387-390,395.[doi:DOI:10.3969/j.issn.1000-7377.2022.04.001]
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去细胞异种神经支架对大鼠足底创面触觉功能重建的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年4期
页码:
387-390,395
栏目:
基础研究
出版日期:
2022-04-05

文章信息/Info

Title:
Effect and mechanism of acellular xenogeneic nerve scaffold on reconstruction of tactile function of rat plantar wound surface
作者:
樊 力; 冯 亮
(陕西省核工业二一五医院手足外科,陕西 咸阳 712000)
Author(s):
FAN Li; FENG Liang
(Department of Hand and Foot Surgery,No.215 Hospital of Shaanxi Nuclear Industry,Xianyang 712000,China)
关键词:
去细胞异种神经支架;  足底创面;  细胞角蛋白-20;  代谢型谷氨酸受体5;  触觉功能;  大鼠
Keywords:
Acellular xenogeneic nerve scaffold;  Plantar wound surface;  Cytokeratin-20;  Metabotropic glutamate receptor 5;  Tactile function;  Rat
分类号:
R 332
DOI:
DOI:10.3969/j.issn.1000-7377.2022.04.001
文献标志码:
A
摘要:
目的:探究去细胞异种神经支架对大鼠足底创面触觉功能重建的影响及机制。方法:将大鼠足底创面模型(n=48)随机平分为模型组、干细胞组、神经支架组三组。模型组不进行治疗,干细胞组将肌源性干细胞(浓度为107个/ml)细胞悬液注射到创伤部位,神经支架组在干细胞组治疗的基础上以注射植入后的细胞异种神经支架进行桥接。结果:干细胞组、神经支架组治疗第7天与第14天的机械痛阈高于模型组(均P<0.05),神经支架组高于干细胞组(P<0.05)。干细胞组、神经支架组治疗第7天与第14天的足底神经指数与运动神经传导速度高于模型组(均P<0.05),神经支架组高于干细胞组(P<0.05)。干细胞组、神经支架组治疗第14天与第21天的有髓神经纤维数量高于模型组(均P<0.05),神经支架组高于干细胞组(P<0.05)。干细胞组、神经支架组治疗第14天与第21天的细胞角蛋白-20(CK-20)与代谢型谷氨酸受体5(mGluR5)蛋白相对表达水平低于模型组(均P<0.05),神经支架组低于干细胞组(P<0.05)。结论:去细胞异种神经支架在大鼠足底创面的应用能抑制CK-20与mGluR5蛋白的表达,有利于触觉功能重建,提高足底神经指数与运动神经传导速度,增加有髓神经纤维数量。
Abstract:
Objective:To explore and study the effect and mechanism of acellular xenogeneic nerve scaffold on the tactile function reconstruction of rat plantar wound surface.Methods:The rat plantar wound surface model(n=48)were randomly equally divided into model group,stem cell group,and nerve scaffold group.The model group was not treated.In the stem cell group,muscle-derived stem cells(at a concentration of 107 cells/ml)were injected into the wound site.The nerve scaffold group was bridged by injecting the implanted cell heterogeneous nerve scaffold on the basis of the treatment of the stem cell group.Results:The mechanical pain threshold of the stem cell group and the nerve scaffold group on the 7th and 14th day of treatment were higher than that of the model group,and the nerve scaffold group was higher than the stem cell group(all P<0.05).The plantar nerve index and motor nerve conduction velocity of the stem cell group and the nerve scaffold group were higher on the 7th and 14th day of treatment than that of the model group,and the nerve scaffold group was higher than the stem cell group(all P<0.05).The number of myelinated nerve fibers in the stem cell group and the nerve scaffold group were higher on the 14th and 21st day of treatment than that in the model group,and the nerve scaffold group was higher than the stem cell group(all P<0.05).The relative expression levels of cytokeratin-20(CK-20)and metabotropic glutamate receptor 5(mGluR5)proteins on the 14th and 21st day of treatment in the stem cell group and nerve scaffold group were lower than those in the model Group,and the nerve scaffold group was lower than the stem cell group(all P<0.05).Conclusion:The application of acellular xenogeneic nerve scaffold on rat plantar wound surface can inhibit the expression of CK-20 and mGluR5 proteins,facilitate the reconstruction of tactile function,improve plantar nerve index and motor nerve conduction velocity,and increase the number of myelinated nerve fibers.

参考文献/References:

[1] 王广军,张宇沁,王瑞红,等.大鼠右侧足底注射C48/80复合物对双侧足底皮肤血流影响的实验研究[J].陕西中医,2010,31(8):1070-1073.
[2] 牛永强,邢俸郡.双极射频技术结合活血止痛汤浴足治疗跖筋膜炎临床研究[J].陕西中医,2019,40(11):1602-1604.
[3] 喻姿瑞,田 钊,张文韬,等.Ilizarov短缩-延长技术治疗小腿节段性毁损伤疗效观察[J].陕西医学杂志,2020,49(12):1638-1641.
[4] 张美玲,王 新,李 波,等.BMSC外泌体调控STAT3/GAP43通路促进脊髓后索损伤大鼠感觉传导功能恢复[J].承德医学院学报,2021,38(2):91-95.
[5] 刘明昊,于才勇,刘 玲,等.骨髓间充质干细胞治疗脊髓损伤应用方法的多样性[J].医学综述,2021,27(2):226-232.
[6] 王健豪,刘 洋,付玄昊,等.3D打印组织工程支架联合骨髓间充质干细胞移植修复脊髓损伤[J].中华骨科杂志,2021,41(6):376-385.
[7] Luo L,He Y,Jin L,et al.Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries[J].Bioact Mater,2021,6(3):638-654.
[8] Lv B,Zhang X,Yuan J,et al.Biomaterial-supported MSC transplantation enhances cell-cell communication for spinal cord injury[J].Stem Cell Res Ther,2021,12(1):36-42.
[9] 刘效谷,吴杰聪,李 芳,等.植物来源神经导管负载EMSCs移植修复大鼠坐骨神经损伤[J].神经解剖学杂志,2021,37(4):383-391.
[10] Ji H,Gu J,Song X,et al.A nerve growth factor persistent delivery scaffold seeded with neurally differentiated bone marrow mesenchymal stem cells promoted the functional recovery of spinal cord injury in rats[J].Am J Transl Res,2021,13(4):2127-2142.
[11] Kunisaki A,Kodama A,Ishikawa M,et al.Carbon-nanotube yarns induce axonal regeneration in peripheral nerve defect[J].ACS Biomater Sci Eng,2021,11(1):19562.
[12] 燕春州.间充质干细胞治疗神经病理性疼痛的研究进展[J].济宁医学院学报,2020,43(6):428-431.
[13] Bai YR,Lai BQ,Han WT,et al.Decellularized optic nerve functional scaffold transplant facilitates directional axon regeneration and remyelination in the injured white matter of the rat spinal cord[J].Prog Biomater,2021,16(11):2276-2283.
[14] 李 浩,杨 振,高仓健,等.支架材料在组织工程技术修复半月板损伤中的应用进展[J].中国医药生物技术,2020,15(6):623-629.
[15] 程 军,李晓红,王景景,等.用于组织工程支架研究脑损伤动物模型的建立[J].中华实验外科杂志,2019,36(6):1132-1135.
[16] 陈平波,徐晓峰,周焕高,等.SHH缓释的脊髓去细胞支架促进大鼠脊髓损伤修复[J].神经解剖学杂志,2018,34(2):203-209.
[17] Chinen T,Yamazaki K,Hashimoto K,et al.Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly[J].J Cell Biol,2021,220(2):991-998.
[18] 李长明,邵荣学,邓小梅,等.尿液细胞来源iPSCs-NSCs联合3D打印支架移植修复大鼠急性脊髓损伤的研究[J].浙江医学,2021,43(3):238-243,后插1.
[19] 石 强,徐建广.间充质干细胞来源外泌体在脊髓损伤治疗中的研究进展[J].国际骨科学杂志,2021,42(5):290-294.
[20] 王美婷,赵 琪,张艺哲,等.背根神经节在药物神经毒性体外评价中的应用[J].中国药理学与毒理学杂志,2021,35(4):305-311.
[21] Zaminy A,Sayad-Fathi S,Kasmaie FM,et al.Decellularized peripheral nerve grafts by a modified protocol for repair of rat sciatic nerve injury[J].Neural Regen Res,2021,16(6):1086-1092.

备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2019SF-209)
更新日期/Last Update: 2022-04-07