[1]潘 婉,李 伟,徐永君,等.先天性心脏病患儿血清微小RNA-148b-3p表达水平及临床意义[J].陕西医学杂志,2022,51(3):334-337.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.016]
　PAN Wan,LI Wei,XU Yongjun,et al.Expression of serum miR-148b-3p in children with congenital heart disease and its clinical significance[J].,2022,51(3):334-337.[doi:DOI:10.3969/j.issn.1000-7377.2022.03.016]
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先天性心脏病患儿血清微小RNA-148b-3p表达水平及临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年3期
页码:
334-337
栏目:
临床研究
出版日期:
2022-03-05

文章信息/Info

Title:
Expression of serum miR-148b-3p in children with congenital heart disease and its clinical significance
作者:
潘 婉1; 李 伟2; 徐永君1; 孙 婧1; 张勇刚3; 郑永强4
(1.宜昌市第二人民医院 三峡大学第二人民医院儿科,湖北 宜昌 443000; 2.宜昌市第二人民医院 三峡大学第二人民医院心血管内科,湖北 宜昌 443000; 3.汉川市人民医院儿科,湖北 汉川 431699; 4.宜昌市第二人民医院 三峡大学第二人民医院神经内科,湖北 宜昌 443000)
Author(s):
PAN Wan; LI Wei; XU Yongjun; SUN Jing; ZHANG Yonggang; ZHENG Yongqiang
(Department of Pediatrics,the Second People's Hospital of Yichang City,Yichang 443000,China)
关键词:
先天性心脏病;  微小RNA-148b-3p;  心功能;  相关性;  预测价值
Keywords:
Congenital heart disease;  miR-148b-3p;  Cardiac function;  Correlation;  Predictive value
分类号:
R 541.1
DOI:
DOI:10.3969/j.issn.1000-7377.2022.03.016
文献标志码:
A
摘要:
目的:检测先天性心脏病患儿血清微小RNA(miR)-148b-3p表达水平,探究其临床意义。方法:选取先天性心脏病患儿150例为研究对象(试验组),根据患儿情况又分为单纯房间隔缺损组(48例)、单纯室间隔缺损组(37例)以及复合组(65例)。选取同期进行健康体检的儿童150例为对照组。采用实时荧光定量PCR(qRT-PCR)检测两组儿童血清miR-148b-3p表达水平。采用全自动生化分析仪检测两组儿童血清肌酸激酶同工酶(CK-MB)水平。采用酶联免疫吸附(ELISA)法检测两组儿童血清中肌钙蛋白I(cTnI)水平。采用超声诊断仪检测左心室射血分数(LVEF)、左心室舒张末期容积指数(LVEDVI)、左心室收缩末期室壁应力(LVESWS)水平。采用Pearson法分析先天性心脏病患儿血清miR-148b-3p表达水平与心功能指标的相关性。采用受试者工作特征(ROC)曲线分析miR-148b-3p水平对先天性心脏病患儿的早期预测价值。结果:与对照组相比,试验组患儿miR-148b-3p、cTnI、CK-MB、LVEDVI、LVESWS水平显著升高,LVEF显著降低(均P<0.05)。单纯房间隔缺损组和单纯室间隔缺损组患儿血清miR-148b-3p水平显著高于复合组(均P<0.05),但单纯房间隔缺损组患儿血清miR-148b-3p水平与单纯室间隔缺损组比较,差异无统计学意义(P>0.05)。Pearson相关性分析显示,先天性心脏病患儿血清miR-148b-3p水平与cTnI、CK-MB、LVEDVI、LVESWS呈正相关,与LVEF呈负相关(均P<0.05)。ROC曲线分析显示,miR-148b-3p水平预测先天性心脏病患儿的曲线下面积为0.873,截断值为1.28,敏感度为81.33%,特异度为85.67%。结论:先天性心脏病患儿血清miR-148b-3p表达水平较高,且与先天性心脏病患儿心功能指标具有相关性,可以作为预测先天性心脏病患儿及评估其病情的参考指标。
Abstract:
Objective:To detect the serum miR-148b-3p expression level in children with congenital heart disease and explore its clinical significance.Methods:150 children with congenital heart disease were selected as the research object(experimental group).According to the situation of children,they were divided into simple atrial septal defect group(48 cases),simple ventricular septal defect group(37 cases)and combination group(65 cases).150 children who underwent physical examination in the same period were selected as the control group.The expression level of serum miR-148b-3p in the two groups was detected by qRT-PCR.The level of serum CK-MB was detected by automatic biochemical analyzer.The level of serum cTnI in the two groups was detected by ELISA.LVEF,LVEDVI and LVESWS were measured by ultrasonic diagnostic instrument.Pearson method was used to analyze the correlation between serum miR-148b-3p expression level and cardiac function indexes in children with congenital heart disease.The ROC curve was used to analyze the early predictive value of miR-148b-3p level in children with congenital heart disease.Results:Compared with the control group,the levels of miR-148b-3p,cTnI,CK-MB,LVEDVI and LVESWS in the experimental group were significantly higher and LVEF was significantly lower(all P<0.05).The level of serum miR-148b-3p in simple atrial septal defect group and simple ventricular septal defect group was significantly higher than that in combination group(all P<0.05),but there was no significant difference between simple atrial septal defect group and simple ventricular septal defect group(P>0.05).Pearson correlation analysis showed that the level of serum miR-148b-3p in children with congenital heart disease was positively correlated with cTnI,CK-MB,LVEDVI and LVESWS,and negatively correlated with LVEF(all P<0.05).ROC curve analysis showed that the area under the curve predicted by miR-148b-3p level in children with congenital heart disease was 0.873,the cut-off value was 1.28,the sensitivity was 81.33%,and the specificity was 85.67%.Conclusion:The expression level of serum miR-148b-3p in children with congenital heart disease is high,and it is correlated with the indexes of cardiac function in children with congenital heart disease.It can be used as a reference index for predicting children with congenital heart disease and evaluating their condition.

参考文献/References:

[1] 陈伟敏,管咏梅,俞红云,等.先天性心脏病患儿疫苗接种的调查研究[J].中国心血管病研究,2021,19(7):644-649.
[2] António M.New-generation multidetector computed tomography technology for the management of congenital heart disease in children:Now we can![J].Rev Port Cardiol,2021,40(8):591-593.
[3] 马 威,魏 波,张慧萍.染色体微阵列分析技术在先天性心脏病胎儿产前遗传学诊断中的应用价值[J].陕西医学杂志,2020,49(11):1436-1439.
[4] 徐珂珂.彩色超声在胎儿先天性心脏病临床诊断中的应用价值[J].哈尔滨医药,2021,41(3):81-82.
[5] 张 铭,张新望,艾 静,等.胃癌患者血浆微小RNA-100表达水平及其临床意义[J].陕西医学杂志,2020,49(7):893-896.
[6] Huang Z,Ma Y,Zhang P,et al.Long non-coding RNA H19 confers resistance to gefitinib via miR-148b-3p/DDAH1 axis in lung adenocarcinoma[J].Anticancer Drugs,2020,31(1):44-54.
[7] Dai W,He J,Zheng L,et al.MiR-148b-3p,miR-190b,and miR-429 regulate cell progression and act as potential biomarkers for breast cancer[J].J Breast Cancer,2019,22(2):219-236.
[8] 李 明,刘 勇,董 海,等.靶向激活miR-148b-3p对缺血性脑卒中小鼠脑功能的影响及机制[J].成都医学院学报,2020,15(4):432-437.
[9] 郝玉青,刘艳丽,王芳芳,等.老年急性缺血性脑卒中患者血清miR-150-5p及miR-148b-3p的表达及其临床意义[J].国际神经病学神经外科学杂志,2020,47(2):116-120.
[10] Willinger L,Brudy L,Meyer M,et al.Prognostic value of non-acute high sensitive troponin-T for cardiovascular morbidity and mortality in adults with congenital heart disease:A systematic review[J].J Cardiol,2021,78(3):206-212.
[11] 李依霖,陈京立.先天性心脏病患儿运动康复的研究进展[J].中华现代护理杂志,2018,24(12):1482-1485.
[12] 张孝兴,李 倩,康淑红,等.炎琥宁配合持续气道正压通气治疗先天性心脏病合并重症肺炎患儿的临床意义[J].陕西中医,2015,36(11):1501-1504.
[13] 李秋花.预见性护理对先天性心脏病患儿并发症发生率及家属自我效能的影响研究[J].中外医学研究,2021,19(22):99-102.
[14] 沈海霞.先天性心脏病患儿血清miR-133a的检测水平及临床意义[J].中国妇幼保健,2021,36(8):1787-1789.
[15] Yang S,Guo J,Zhou L,et al.MiR-148b-3p,miR-337-5p and miR-423-5p expression in alveolar ridge atrophy and their roles in the proliferation and apoptosis of OMMSCs[J].Exp Ther Med,2018,16(6):5334-5342.
[16] Zhang H,Ye Q,Du Z,et al.MiR-148b-3p inhibits renal carcinoma cell growth and pro-angiogenic phenotype of endothelial cell potentially by modulating FGF2[J].Biomed Pharmacother,2018,107:359-367.
[17] Chen MC,Chang TH,Chang JP,et al.Circulating miR-148b-3p and miR-409-3p as biomarkers for heart failure in patients with mitral regurgitation[J].Int J Cardiol,2016,222(1):148-154.
[18] 许 辉,沈 杰,谢兰军.miR-148b-3p通过调控Wnt信号通路对胶质瘤细胞侵袭和迁移的影响[J].中华肿瘤杂志,2020,42(7):565-569.
[19] 王嘉明,郭丽敏,郭艳娟,等.miR-148b-3p通过调控CDKN1B表达影响心肌细胞增殖及凋亡的分子机制[J].中国循证心血管医学杂志,2020,12(7):846-850,854.
[20] 龚 化,周治军,卢 童,等.miR-148b-3p在前列腺癌细胞增殖和迁移中的作用机制[J].遵义医学院学报,2018,41(4):443-448.

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备注/Memo

备注/Memo:
基金项目:湖北省卫生健康委员会科研项目(WJ2019M065)
更新日期/Last Update: 2022-03-04