[1]陈 丹,王文静,王庆雅,等.生物发光成像技术在肿瘤细胞活体可视化研究中的应用研究进展[J].陕西医学杂志,2022,51(1):111-116.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.028]
　CHEN Dan,WANG Wenjing,WANG Qingya,et al.Application of bioluminescence imaging technology in visualization of cancer cells in vivo[J].,2022,51(1):111-116.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.028]
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生物发光成像技术在肿瘤细胞活体可视化研究中的应用研究进展
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年1期
页码:
111-116
栏目:
综 述
出版日期:
2022-01-05

文章信息/Info

Title:
Application of bioluminescence imaging technology in visualization of cancer cells in vivo
作者:
陈 丹; 王文静; 王庆雅; 曾 鋆; 詹勇华
(西安电子科技大学生命科学技术学院分子与神经影像教育部工程研究中心,陕西 西安 710126)
Author(s):
CHEN Dan; WANG Wenjing; WANG Qingya; ZENG Yun; ZHAN Yonghua
(Engineering Research Centerof Molecular and Neuroimaging,Ministry of Education,School of Life Science and Technology,Xidian University,Xi'an 710126,China)
关键词:
生物发光成像;  动物模型;  荧光素酶;  报告基因;  可视化;  肿瘤
Keywords:
Bioluminescence imaging;  Animal model;  Luciferase;  Reporter gene;  Visualization;  Cancer
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2022.01.028
文献标志码:
A
摘要:
在肿瘤研究中,以小鼠和斑马鱼为主的动物模型实验作为介于体外细胞实验和临床试验之间重要的中间环节,往往很受青睐。近年来,挑选一些在肿瘤细胞进程中有特殊活性的启动子,构建这类启动子调控的荧光素酶报告基因,或者将荧光素酶基因的cDNA插入到肿瘤特异性表达的基因调控序列中,建立转基因小鼠和斑马鱼动物模型,随后通过生物发光成像技术(BLI),可以在活体动物模型中实现肿瘤细胞进程的可视化研究,包括对肿瘤细胞增殖、凋亡、迁移和免疫反应的实时监控。现综述利用BLI技术和活体动物成像模型在实现肿瘤细胞进程的可视化和确定肿瘤早期发展阶段方面的研究进展,及其用于不同组织器官间活动和肿瘤治疗方面的发展和应用。
Abstract:
As an important intermediate link between in vitro cell experiments and clinical experiments,animal models such as mice and zebrafish models are often preferred in cancer research.In recent years,some promoters with special activity in tumor progression have been selected to construct luciferase reporter genes.Moreover,to establish the transgenic mouse and zebrafish animal models,the cDNA of luciferase gene has been inserted into the regulation sequence of genes which have extra expression in cancer.Subsequently,through Bioluminescence Imaging(BLI),it makes it possible to visualization of tumor progression in vivo in living animal models,including real-time monitoring of cancer cell proliferation,apoptosis,migration and immune response.In this review,we summarize the advances in the use of BLI technology and in vivo animal imaging models to visualize cancer cell and identify early stages of tumor progression,as well as the development and application of BLI technology in the movement of different tissues and organs and cancer therapy.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(91859109,32001074); 陕西省自然科学基础研究计划项目(2020JM-209); 人因工程重点实验室基础研究基金对外开放基金资助项目(SYFD061908K); 中央高校基本科研业务费资助项目(JB211206)
更新日期/Last Update: 2022-01-09