[1]张霞婧,井紫薇,朱芳芸,等.大麻素受体2激动剂在谷氨酸氧化应激损伤中保护作用实验研究[J].陕西医学杂志,2022,51(1):25-28.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.006]
　ZHANG Xiajing,JING Ziwei,ZHU Fangyun,et al.Protective effects of cannabinoid receptor 2 agonists on glutamate oxidative stress injury[J].,2022,51(1):25-28.[doi:DOI:10.3969/j.issn.1000-7377.2022.01.006]
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大麻素受体2激动剂在谷氨酸氧化应激损伤中保护作用实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年1期
页码:
25-28
栏目:
基础研究
出版日期:
2022-01-05

文章信息/Info

Title:
Protective effects of cannabinoid receptor 2 agonists on glutamate oxidative stress injury
作者:
张霞婧1; 井紫薇2; 朱芳芸1; 小 辉1; 邵勇平1; 郑 凌1
(1.西安市人民医院 西安市第四医院麻醉科,陕西 西安 710004; 2.空军军医大学唐都医院麻醉科,陕西 西安 710038)
Author(s):
ZHANG Xiajing; JING Ziwei; ZHU Fangyun; XIAO Hui; SHAO Yongping; ZHENG Ling
(Department of Anesthesiology,Xi'an People's Hospital,Xi'an 710004,China)
关键词:
大麻素;  大麻素受体2;  谷氨酸;  小神经胶质细胞;  神经元;  氧化应激
Keywords:
Cannabinoid;  CB2 receptor;  Glutamate;  Microglia;  Neuron;  Oxidative stress
分类号:
R 91
DOI:
DOI:10.3969/j.issn.1000-7377.2022.01.006
文献标志码:
A
摘要:
目的:评价大麻素受体2(CB2受体)通过抗氧化应激机制在谷氨酸对共培养小胶质细胞和神经元损伤中的保护作用。方法:采用随机数字表法将培养的N9小胶质细胞和HT22神经元分为四组(n=24):对照组(Con组)、谷氨酸组(Glu组)、CB2受体激动剂AM1241+谷氨酸组(AM1241+Glu组)和AM1241+CB2受体拮抗剂AM630+谷氨酸组(AM1241+AM630+Glu组)。Con组正常培养24 h; Glu组用含10 mmol/L谷氨酸的培养基孵育24 h; AM1241+Glu组用含2 μmol/L AM1241和10 mmol/L谷氨酸的培养基孵育24 h; AM1241+AM630+Glu组含2 μmol/L AM1241、6 μmol/L AM630及10 mmol/L谷氨酸的培养基孵育24 h。采用MTT法测定细胞活力,采用化学比色法测定乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)的含量。结果:与Con组比较,Glu组和AM1241+AM630+Glu组细胞活力和SOD活性降低,LDH活性和MDA含量升高(均P<0.05),AM1241+Glu组细胞活力和SOD活性降低,LDH含量升高(均P<0.05); 与Glu组比较,AM1241+Glu组细胞活力和SOD活性升高,LDH活性和MDA含量降低(均P<0.05); 与AM1241+Glu组比较,AM1241+AM630+Glu组细胞活力和SOD活性降低,LDH活性和MDA含量升高(均P<0.05)。结论:CB2受体激活减轻谷氨酸诱发小胶质细胞和神经元损伤的机制与抗氧化作用有关。
Abstract:
Objective:To observe the effect of cannabinoid receptor 2(CB2)against glutamate damage to co-cultured microglia and neurons through anti-oxidative stress mechanism.Methods:Co-cultured microglia and neurons were divided into four groups(n=24 each)using a random number table:control group(Con group),glutamate group(Glu group),CB2 agonist AM1241+glutamate group(AM1241+Glu group)and AM1241+CB2 antagonist AM630 group(AM1241+AM630+Glu group).Cells in Con group were cultured with normal medium for 24 hours.Cells in Glu group were cultured with medium contained 10 mmol/L glutamate for 24 hours.Cells in AM1241+Glu group were cultured with medium contained 2 μmol/L AM1241 and 10 mmol/L glutamate for 24 hours.Cells in AM1241+AM630+Glu group were cultured with medium contained 2 μmol/L AM1241,6 μmol/L AM630 and 10 mmol/L glutamate for 24 hours.The cell viability was measured by MTT assay,the activity of lactate dehydrogenase(LDH),superoxide dismutase(SOD)and the content of malondialdehyde(MDA)were detected by chemical colorimetry.Results:Compared with Con group,the cell viability and SOD activity were decreased,LDH activity and MDA level were increased in Glu group and AM1241+AM630+Glu group(all P<0.05); the cell viability and SOD activity were decreased,LDH activity was increased in AM1241+Glu group(all P<0.05).Compared with Glu group,the cell viability and SOD activity were increased,LDH activity and MDA level were decreased in AM1241+Glu group(all P<0.05).Compared with AM1241+Glu group,the cell viability and SOD activity were decreased,LDH activity and MDA level were increased in AM1241+AM630+Glu group(all P<0.05).Conclusion:The mechanism of CB2 receptor activation in reducing microglia and neuron injury induced by glutamate is related to antioxidant effect.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81903261); 陕西省自然科学基础研究计划项目(2020JQ-957); 西安市科技计划项目[2019115413YX009SF042(2)]; 西安市第四医院孵化基金资助项目(LF-4)
更新日期/Last Update: 2022-01-09