[1]崔 凯,白峻峰,公孙鑫,等.Wiskott-Aldrich综合征蛋白家族成员3对非小细胞肺癌侵袭转移的影响及机制研究[J].陕西医学杂志,2021,50(12):1505-1509.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.009]
　CUI Kai,BAI Junfeng,GONGSUN Xin,et al.Effect and mechanism of WASF3-AS1 on invasion and metastasis of non-small cell lung cancer[J].,2021,50(12):1505-1509.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.009]
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Wiskott-Aldrich综合征蛋白家族成员3对非小细胞肺癌侵袭转移的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年12期
页码:
1505-1509
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Effect and mechanism of WASF3-AS1 on invasion and metastasis of non-small cell lung cancer
作者:
崔 凯1; 白峻峰1; 公孙鑫1; 支亚男1; 王壮壮1; 姜 琨1; 贾 明2
(1.西安国际医学中心医院胸腔外科,陕西 西安 710100; 2.陕西省中医药研究院, 陕西 西安 710003)
Author(s):
CUI Kai; BAI Junfeng; GONGSUN Xin; ZHI Ya'nan; WANG Zhuangzhuang; JIANG Kun; JIA Ming
(Department of Thoracic Surgery,Xi'an International Medical Center Hospital,Xi'an 710100,China)
关键词:
非小细胞肺癌;  Wiskott-Aldrich综合征蛋白家族成员3;  赖氨酸特异性组蛋白去甲基化酶1;  组蛋白去乙酰化酶6;  侵袭转移;  机制
Keywords:
Non-small cell lung cancer;  ASF3-AS1;  Lysine-specific histone demethylase 1;  Histone demethylase 6;  Invasion and metastasis;  Mechanism
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2021.12.009
文献标志码:
A
摘要:
目的:探究Wiskott-Aldrich综合征蛋白家族成员3(WASF3-AS1)对非小细胞肺癌(NSCLC)侵袭转移的影响及机制。方法:选取26例临床不同病理分期NSCLC患者的癌组织和癌旁组织,以及NSCLC细胞系NCI-H1975细胞和正常肺支气管上皮细胞系HBE细胞为研究对象。实时荧光定量PCR(qRT-PCR)检测上述临床组织和细胞系中WASF3-AS1、赖氨酸特异性组蛋白去甲基化酶1(LSD1)、组蛋白去乙酰化酶6(HDAC6)、E-钙黏蛋白(E-ca)、N-钙黏蛋白(N-ca)的表达。采用CCK-8实验检测细胞增殖。采用划痕实验检测细胞迁移能力。采用Transwell实验检测细胞侵袭能力。采用Western blot检测LSD1、HDAC6、蛋白酪氨酸磷酸酶(PTEN)、蛋白激酶B(AKT)、E-ca和N-ca的表达。结果:与对照组比较,在NSCLC组织和细胞中,WASF3-AS1、LSD1、HDAC6、PTEN和E-ca低表达,AKT和N-ca高表达(均P<0.05); WASF3-AS1过表达能够促进LSD1和HDAC6并抑制PTEN/AKT信号,进而抑制NCI-H1975细胞增殖、迁移、侵袭和上皮间充质转化(均P<0.05)。结论:ASF3-AS1可能通过招募LSD1和HDAC6到靶基因PTEN启动子区发挥抑制NSCLC细胞侵袭转移作用。
Abstract:
Objective:To investigate the effect and mechanism of WASF3-AS1 on the invasion and metastasis of non-small-cell lung cancer(NSCLC).Methods:The cancer tissues and adjacent tissues of 26 NSCLC patients with different clinical pathological stages,as well as NSCLC cell line NCI-H1975 cells and normal lung bronchial epithelial cell line HBE cells were selected as the research objects.Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect the expression of WASF3-AS1,lysine-specific histone demethylase 1(LSD1),histone deacetylase 6(HDAC6),E-cadherin(E-ca)and N-cadherin(N-ca)in the above clinical tissues and cell lines.The CCK-8 experiment was used to detect cell proliferation.Scratch-wound experiment was used to detect cell migration ability.Transwell experiment was used to detect cell invasion ability.Western blot was used to detect the expression of LSD1,HDAC6,protein tyrosine phosphatase(PTEN),protein kinase B(AKT),E-ca and N-ca.Results:Compared with the control group,in NSCLC tissues and cells,WASF3-AS1,LSD1,HDAC6,PTEN and E-ca were lowly expressed,and AKT and N-ca were highly expressed(all P<0.05); Overexpression of WASF3-AS1 could promote LSD1 and HDAC6 and inhibit PTEN/AKT signaling,thereby inhibiting the proliferation,migration,invasion and epithelial-mesenchymal transformation of NCI-H1975 cells(all P<0.05).Conclusion:ASF3-AS1 may inhibit the invasion and metastasis of NSCLC cells by recruiting LSD1 and HDAC6 to the promoter region of the target gene PTEN.

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更新日期/Last Update: 2021-12-07