[1]刘烈东,张鹏贵,白晓兵.维生素K2对去卵巢大鼠股骨干骨折愈合及骨密度影响的实验研究[J].陕西医学杂志,2021,50(12):1501-1504.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.008]
　LIU Liedong,ZHANG Penggui,BAI Xiaobing.Effect of vitamin K2 on fracture healing and bone density in ovariectomized femoral shaft fracture model rats[J].,2021,50(12):1501-1504.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.008]
点击复制

维生素K2对去卵巢大鼠股骨干骨折愈合及骨密度影响的实验研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年12期
页码:
1501-1504
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Effect of vitamin K2 on fracture healing and bone density in ovariectomized femoral shaft fracture model rats
作者:
刘烈东1; 张鹏贵1; 白晓兵2
(1.榆林市第一医院骨科,陕西 榆林 719000; 2.榆林市第一医院手足显微外科,陕西 榆林 719000)
Author(s):
LIU Liedong; ZHANG Penggui; BAI Xiaobing
(Department of Orthopaedics,the First People's Hospital of Yulin,Yulin 719000,China)
关键词:
股骨干骨折;  维生素K2;  卵巢切除;  骨折愈合;  骨密度;  骨代谢
Keywords:
Femoral shaft fracture;  Vitamin K2;  Oophorectomize;  Fracture healing;  Bone density;  Bone metabolism
分类号:
R 683.42
DOI:
DOI:10.3969/j.issn.1000-7377.2021.12.008
文献标志码:
A
摘要:
目的:研究维生素K2对去卵巢股骨干骨折模型大鼠骨折愈合及骨密度的影响。方法:将90只雌性SD大鼠随机分为假手术组、骨折组及干预组,每组30只。三组大鼠均实施卵巢切除干预,其中骨折组与干预组均建立股骨干骨折模型。干预组采用维生素K2灌胃,假手术组与骨折组均予以等量0.9%氯化钠溶液,连续干预5周。比较三组大鼠骨折愈合状况、骨密度[包括全长骨密度(tBMD)、中1/3段骨密度(mBMD)以及远1/3段骨密度(dBMD)],以及骨代谢指标[包括骨钙素(BGLAP)、碱性磷酸酶(ALP)及抗酒石醋酸酸性磷酸酶(TRACP)]、血清血管内皮生长因子(VEGF)和骨形成蛋白-2(BMP-2)表达水平。结果:X线检查发现,骨折组大鼠不完全愈合,而干预组存在明显的骨痂组织形成、重塑以及骨愈合。骨折组大鼠右股骨tBMD、mBMD及dBMD均低于假手术组,而干预组各项指标水平均高于骨折组(均P<0.05)。骨折组大鼠BGLAP、ALP及抗TRACP水平均明显高于骨折组,而干预组大鼠各项指标水平均明显低于骨折组(均P<0.05)。骨折组大鼠血清VEGF、BMP-2表达水平均明显低于假手术组,而干预组VEGF、BMP-2表达水平均高于骨折组(均P<0.05)。结论:维生素K2可促进去卵巢股骨干骨折模型大鼠的骨折愈合并增加骨密度。
Abstract:
Objective:To study the effect of vitamin K2 on the fracture healing and bone density in ovariectomized femoral shaft fracture model rats.Methods:90 female SD rats were randomly divided into sham operation group,fracture group and intervention group,with 30 rats in each group.Ovariectomies were performed in all three groups,and femoral shaft fracture models were established in the fracture group and the intervention group.The intervention group was given intragastric administration of vitamin K2,and the sham operation group and the fracture group were given the same amount of 0.9% sodium chloride solution,and all the rats were treated for 5 weeks.The fracture healing status,bone density(including tBMD,mBMD and dBMD),and the expression levels of bone metabolism indexes(including BGLAP,ALP and TRACP),serum VEGF and BMP-2 in the three groups of rats were compared.Results:X-ray examination showed that the fracture group rats were not completely healed,and the intervention group had obvious callus formation,remodeling and bone healing.The tBMD,mBMD and dBMD of right femur in the fracture group were lower than those in the sham operation group,and the levels of the above indicators in the intervention group were higher than those in the fracture group(all P<0.05).The BGLAP,ALP and TRACP levels in the fracture group were significantly higher than those in the fracture group,and the levels of the above indexes in the intervention group were significantly lower than those in the fracture group(all P<0.05).Serum VEGF and BMP-2 expression levels in the fracture group were significantly lower than those in the sham operation group,and the expression levels of VEGF and BMP-2 in the intervention group were higher than those in the fracture group(all P<0.05).Conclusion:Vitamin K2 can promote fracture healing and increase bone density in ovariectomized femoral shaft fracture model rats.

参考文献/References:

[1] Li J,Rai S,Ze R,et al.The optimal choice for length unstable femoral shaft fracture in school-aged children:A comparative study of elastic stable intramedullary nail and submuscular plate[J].Medicine(Baltimore),2020,99(25):20796-20797.
[2] Hussain M,Kakazu R,Jimenez A,et al.proximal cortical breach after retrograde femoral nailing for femoral shaftfracture:A case report[J].JBJS Case Connect,2020,10(1):388-389.
[3] 李成磊.股骨干骨折后戒烟能减少骨不连[J].中华关节外科杂志:电子版,2020,14(1):111-114.
[4] 龙如超,朱建军.利塞膦酸钠对大鼠骨质疏松性骨折愈合的影响[J].中国临床药理学杂志,2020,36(10):1299-1301.
[5] 毛未贤,张萌萌,马倩倩,等.维生素K联合骨化三醇、钙尔奇D治疗男性骨质疏松的疗效观察[J].中国骨质疏松杂志,2020,26(2):166-169.
[6] 温霜威,武青梅.有氧运动联合左旋甲状腺素与维生素D3改善亚临床甲减大鼠骨质疏松的作用[J].中国组织工程研究,2020,24(26):4118-4124.
[7] 刘 颖,孙昌俊,王 乐,等.甲状旁腺激素联合阿仑膦酸钠对大鼠骨质疏松性骨折骨痂血管形成的影响[J].中国骨质疏松杂志,2019,25(5):669-673.
[8] Lu Y,Wang Y,Song Z,et al.Treatment comparison of femoral shaft with femoral neck fracture:A meta-analysis[J].J Orthop Surg Res,2020,15(1):19-20.
[9] Tan M,Siow JWX,Kwek EBK.Intramedullary nailing of abnormally bowed atypical femoral shaft fractures:Surgical technique[J].Arch Orthop Trauma Surg,2020,140(3):353-357.
[10] Park KH,Park BK,Oh CW,et al.Overgrowth of the femur after internal fixation in children with femoral shaftfracture:A multicenter study[J].J Orthop Trauma,2020,34(3):90-95.
[11] 杨雨润,李子荣,孙 伟,等.顺行髓内钉治疗股骨干骨折后发生股骨头坏死的原因分析[J].中国骨质疏松杂志,2019,25(2):169-173.
[12] 丁 杰,乔旭霞.仙灵骨葆胶囊联合金天格胶囊治疗股骨干骨折骨质疏松症疗效观察[J].西部中医药,2019,32(1):74-76.
[13] 刘黎明,帖利军,史晓薇,等.维生素K与儿童健康关系的研究[J].中国妇幼健康研究,2019,30(9):1039-1047.
[14] 倪梦杉,陈贤哲,陈宇雄,等.治疗骨质疏松和骨折何时联用或介入抗骨质疏松治疗[J].中国组织工程研究,2018,22(4):625-630.
[15] 吴信举,陶周善,陆汉力,等.维生素K2对去卵巢大鼠股骨干骨折愈合影响的实验研究[J].中国骨质疏松杂志,2020,26(2):186-190.
[16] 赵成毅,李 青,梁道臣,等.椎体成形术后应用唑来膦酸联合维生素K2治疗老年性椎体压缩骨折[J].中华关节外科杂志:电子版,2018,12(3):127-130.
[17] 王可可,李思淼,罗 婕,等.骨质疏松症临床实践指南评价及药物综合价值研究[J].中国医院药学杂志,2020,40(1):91-98.
[18] 蒋益忠,夏天爽,辛海量,等.维生素K对成骨细胞骨形成和破骨细胞骨吸收的影响[J].药学实践杂志,2020,38(4):340-345.
[19] 刘 颖,孙昌俊,王 乐,等.甲状旁腺激素联合阿仑膦酸钠对大鼠骨质疏松性骨折骨痂血管形成的影响[J].中国骨质疏松杂志,2019,25(5):669-673.
[20] 安 福,王永刚,刘文忠,等.VEGF在大鼠脊髓损伤后股骨干骨痂形成过程中的表达[J].医学综述,2014,20(1):129-131.
[21] 李 鹏,佘新安,赵东军,等.骨髓间充质干细胞对大鼠骨质疏松股骨干骨折早期愈合影响的实验研究[J].陕西医学杂志,2021,50(7):793-796.
[22] 武 雷.桃仁四物汤加减联合常规西医疗法对创伤骨折患者软组织修复及骨折愈合的影响[J].陕西中医,2016,37(7):780-781.

备注/Memo

备注/Memo:
基金项目:陕西省榆林市科技计划项目(2019YL231)
更新日期/Last Update: 2021-12-07