[1]郑文博,赵 瑛.冬凌草甲素对高脂饮食诱导幼龄大鼠非酒精性脂肪肝的作用及相关机制[J].陕西医学杂志,2021,50(12):1496-1500.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.007]
　ZHENG Wenbo,ZHAO Ying.Effect and mechanism of oridonin on non-alcoholic fatty liver disease induced by high-fat diet in young rats[J].,2021,50(12):1496-1500.[doi:DOI:10.3969/j.issn.1000-7377.2021.12.007]
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冬凌草甲素对高脂饮食诱导幼龄大鼠非酒精性脂肪肝的作用及相关机制

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年12期
页码:
1496-1500
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Effect and mechanism of oridonin on non-alcoholic fatty liver disease induced by high-fat diet in young rats
作者:
郑文博1; 赵 瑛2
(1.陕西中医药大学第二附属医院儿童神经内分泌科,陕西 咸阳 712000; 2.西安医学院附属宝鸡医院消化内科,陕西 宝鸡 721006)
Author(s):
ZHENG Wenbo; ZHAO Ying
(Department of Neuroendocrinology for Children,the Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China)
关键词:
非酒精性脂肪肝;  冬凌草甲素;  高脂饮食;  幼龄大鼠;  核因子E2相关因子2;  氧化应激
Keywords:
Non-alcoholic fatty liver disease;  Oridonin;  High-fat diet;  Young rats;  Nrf2;  Oxidative stress
分类号:
R 575.5
DOI:
DOI:10.3969/j.issn.1000-7377.2021.12.007
文献标志码:
A
摘要:
目的:探讨冬凌草甲素对高脂饮食诱导的幼龄大鼠非酒精性脂肪肝(NAFLD)的作用及相关机制。方法:将56只3周龄SD大鼠随机分为对照组、模型组、冬凌草甲素低剂量组(5 mg/kg)、冬凌草甲素中剂量组(10 mg/kg)和冬凌草甲素高剂量组(20 mg/kg)。比较各组大鼠体重、空腹血糖、空腹血清胰岛素、肝功能指标[谷草转氨酶(AST)、谷丙转氨酶(ALT)]、血脂指标[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]、氧化应激指标[超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和谷胱甘肽过氧化物酶(GSH-Px)]、肝组织HE染色和油红O染色结果,以及肝组织核因子E2相关因子2(Nrf2)、血红素氧合酶1(HO-1)和醌氧化还原酶-1(NQO-1)蛋白的表达。结果:模型组大鼠体重、空腹血糖、空腹血清胰岛素、TC、TG、LDL-C、ALT、AST、LDH和GSH-Px水平高于对照组,HDL-C、SOD水平低于对照组,Nrf2、HO-1、NQO-1蛋白水平低于对照组(均P<0.05)。冬凌草甲素低剂量组大鼠各指标与模型组比较差异无统计学意义(均P>0.05)。冬凌草甲素中剂量组和高剂量组大鼠体重、空腹血糖、空腹血清胰岛素、TC、TG、LDL-C、ALT、AST、LDH和GSH-Px水平低于模型组,HDL-C、SOD水平高于模型组,Nrf2、HO-1、NQO-1蛋白水平高于模型组(均P<0.05)。HE染色和油红O染色结果表明,冬凌草甲素能够显著缓解大鼠肝组织脂肪改变,减少炎性细胞浸润。结论:冬凌草甲素通过激活Nrf2降低氧化应激水平,缓解高脂饮食诱导的幼龄大鼠NAFLD,为临床防治NAFLD提供了依据。
Abstract:
Objective:To investigate the effect and mechanism of oridonin on NAFLD induced by high-fat diet in young rats.Methods:56 SD rats with 3 weeks old were randomly divided into control group,model group,low-dose oridonin group(5 mg/kg),medium-dose oridonin group(10 mg/kg)and high-dose oridonin group(20 mg/kg).The body weight,fasting blood glucose,fasting serum insulin,liver function indexes(AST and ALT),blood lipid indexes(TC,TG,LDL-C, HDL-C),oxidative stress indexes(SOD,LDH and GSH-Px),results of HE and oil red O staining of liver tissue,and the expression of Nrf2,HO-1 and NQO-1 proteins in liver tissue were compared among five groups.Results:The body weight,fasting blood glucose,fasting serum insulin,TC,TG,LDL-C,ALT,AST,LDH and GSH-Px levels in the model group were higher than those in the control group,the levels of HDL-C and SOD were lower than those in the control group,and the levels of Nrf2,HO-1 and NQO-1 protein were lower than those in the control group(all P<0.05).There were no significant differences in above indexes between the low-dose oridonin group and the model group(all P>0.05).The body weight,fasting blood glucose,fasting serum insulin,TC,TG,LDL-C,ALT,AST,LDH and GSH-Px levels in the middle and high-dose oridonin group were lower than those in the model group,the levels of HDL-C and SOD were higher than those in the model group,and the levels of Nrf2,HO-1 and NQO-1 proteins were higher than those in the model group(all P<0.05).HE and oil red O staining showed that oridonin could alleviate the fat changes in rat liver tissue and reduce inflammatory cell infiltration.Conclusion:Oridonin can reduce the level of oxidative stress and alleviate NAFLD induced by high-fat diet in young rats throught activating Nrf2,which provides a basis for clinical prevention and treatment of NAFLD.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2019SF-042)
更新日期/Last Update: 2021-12-07