[1]程 继,王志刚,付 国,等.姜黄素@普朗尼克纳米胶束抑制前列腺癌PC-3细胞生长实验研究[J].陕西医学杂志,2021,50(11):1340-1344.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.005]
　CHENG Ji,WANG Zhigang,FU Guo,et al.Antitumor effect of curcumin@Pluronic nanomicelles on prostate cancer PC-3 cells[J].,2021,50(11):1340-1344.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.005]
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姜黄素@普朗尼克纳米胶束抑制前列腺癌PC-3细胞生长实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年11期
页码:
1340-1344
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Antitumor effect of curcumin@Pluronic nanomicelles on prostate cancer PC-3 cells
作者:
程 继; 王志刚; 付 国; 董青川
(陕西省人民医院泌尿外科,陕西 西安 710068)
Author(s):
CHENG Ji; WANG Zhigang; FU Guo; DONG Qinchuan
(Department of Urinary Surgery,Shaanxi Provincial People's Hospital,Xi'an 710068,China)
关键词:
姜黄素;  胶束;  普朗尼克;  前列腺癌;  抗肿瘤;  PC-3细胞
Keywords:
Curcumin;  Micelles;  Pluronic;  Prostate cancer;  Antitumor;  PC-3 cells
分类号:
R 915
DOI:
DOI:10.3969/j.issn.1000-7377.2021.11.005
文献标志码:
A
摘要:
目的:制备姜黄素@普朗尼克纳米胶束,观察其对前列腺癌PC-3细胞的体内外抗肿瘤活性。方法:溶剂挥发法制备姜黄素@普朗尼克纳米胶束,激光粒度仪测定胶束的粒径分布,HPLC法测定胶束的载药量、包封率及体外释药,观察姜黄素@普朗尼克纳米胶束的细胞摄取; MTT法评价姜黄素胶束的细胞毒性; 并建立裸鼠荷PC-3肿瘤模型,观察肿瘤体积变化,计算肿瘤体积抑制率,评价姜姜黄素@普朗尼克纳米胶束的体内抗肿瘤活性。结果:姜黄素@普朗尼克纳米胶束平均粒径为322 nm,呈圆球状,载药量和包封率分别为(15.7±0.3)%和(89.4±1.2)%,72 h体外释药可达80%,具有一定的缓释效果; 细胞试验显示胶束可显著增加姜黄素细胞内的聚集,抑制PC-3细胞的增殖; 体内抗肿瘤研究显示,胶束显著减小肿瘤体积,有效抑制肿瘤生长,提示该纳米胶束具有一定的体内抗肿瘤作用。结论:姜黄素@普朗尼克纳米胶束具有一定缓释作用,可显著提高姜黄素在细胞内的蓄积水平,增强姜黄素对前列腺癌PC-3细胞的体外抗肿瘤作用,并呈现出一定的体内抗肿瘤效果。
Abstract:
Objective:To prepare curcumin@Pluronic nanomicelles and observe its antitumor activity on prostate cancer PC-3 cells in vivo and in vitro.Methods:The curcumin@Pluronic nanomicelles were prepared by solvent evaporation method.The particle size distribution of the nanomicelles was determined by laser particle size analyzer.The drug loading,encapsulation efficiency and release profile in vitro were determined by HPLC.The cytotoxicity of curcumin@Pluronic micelles was evaluated by MTT method.The nude mouse bearing PC-3 tumor model was established,and the tumor volume change was observed,the tumor volume inhibition rate was calculated,and the anti-tumor activity of curcumin@Pluronic nanomicelles in vivo was evaluated.Results:The average particle size of curcumin@Pluronic nanomicelles was 322 nm.The drug loading and entrapment efficiency were(15.7±0.3)% and(89.4±1.2)%,respectively.The drug release in vitro could reach 80% after 72 hours.The micelles could significantly increase the intracellular accumulation of curcumin and inhibit the proliferation of PC-3 cells.In vivo antitumor studies showed that curcumin@Pluronic nanomicelles could effectively inhibit tumor growth,indicated the nanomicelles had a certain antitumor effect in vivo.Conclusion:The curcumin@Pluronic nanomicelles have a certain sustained-release effect,which can significantly increase the intracellular accumulation of curcumin and enhance the antitumor effect of curcumin on prostate cancer PC-3 cells in vitro,and also showed certain degree of antitumor profile in vivo.

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更新日期/Last Update: 2021-11-05