[1]胡文静,沈红良,张 萌,等.干眼症小鼠泪液中胸腺基质淋巴细胞生成素和白介素18水平变化及相关机制研究[J].陕西医学杂志,2021,50(11):1333-1335,1344.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.003]
　HU Wenjing,SHEN Hongliang,ZHANG Meng,et al.Changes and related mechanisms of thymic stromal lymphopoietin and interleukin-18 in tears of mice with dry eye[J].,2021,50(11):1333-1335,1344.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.003]
点击复制

干眼症小鼠泪液中胸腺基质淋巴细胞生成素和白介素18水平变化及相关机制研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年11期
页码:
1333-1335,1344
栏目:
基础研究
出版日期:
2021-11-05

文章信息/Info

Title:
Changes and related mechanisms of thymic stromal lymphopoietin and interleukin-18 in tears of mice with dry eye
作者:
胡文静1; 沈红良2; 张 萌3; 延新年3
(1.延安市中医医院眼科,陕西 延安716000; 2.西安交通大学第一附属医院检验科,陕西 西安710061; 3.咸阳市第一人民医院青光眼综合眼病中心,陕西 咸阳712000)
Author(s):
HU Wenjing; SHEN Hongliang; ZHANG Meng; YAN Xinnian
(Department of Ophthalmology,Yan'an Hospital of Traditional Chinese Medicine,Yan'an 716000,China)
关键词:
胸腺基质淋巴细胞生成素;  干眼症;  小鼠;  苯扎氯铵;  炎症因子;  泪液
Keywords:
Thymic stromal lymphopoietin;  Dry eye;  Mice;  Benzalkonium chloride;  Inflammatory factor;  Tear
分类号:
R 777.34
DOI:
DOI:10.3969/j.issn.1000-7377.2021.11.003
文献标志码:
A
摘要:
目的:探讨干眼症小鼠泪液中胸腺基质淋巴细胞生成素(TSLP)和白介素18(IL-18)的水平变化及相关机制。方法:64只健康雄性BALB/c小鼠随机分为对照组与模型组各32只。模型组小鼠双眼各滴入5 μl 0.2%苯扎氯铵溶液,每天2次,连续15 d,诱导小鼠干眼症模型,对照组以等剂量的磷酸盐缓冲液滴眼。采用酚红棉线检测泪液分泌量,行角膜荧光素染色和HE染色,记录泪膜破裂时间,采用酶联免疫法检测泪液中IL-18、TSLP水平。结果:两组实验第1天的泪液分泌实验(SIT)、泪膜破裂时间(BUT)、角膜荧光素染色(FL)评分、IL-18、TSLP水平对比差异无统计学意义(均P>0.05),模型组实验第15天的SIT与BUT值低于对照组,也低于实验第1天,对比差异均有统计学意义(均P<0.05); 模型组实验第15天的FL评分、泪液IL-18、TSLP水平均显著高于对照组和实验第1天(均P<0.05)。结论:TSLP在干眼症小鼠泪液中呈现高表达水平,可能抑制神经递质的释放,促进泪液IL-18的表达,降低泪液分泌,从而诱发干眼症的发生。
Abstract:
Objective:To investigate the changes and related mechanisms of thymic stromal lymphopoietin(TSLP)and interleukin-18(IL-18)in tears of mice with dry eye.Methods:64 cases of healthy male BALB/c mice were randomly divided into control group and model group,with 32 cases in each.Mice in the model group were injected into both eyes with 5 μl 0.2% benzalkonium chloride solution,twice a day for 15 days,to induce the mouse dry eye model.The control group was dripped with the same dose of phosphate buffer.The tear secretion was detected by phenol red cotton thread,corneal fluorescein staining and HE staining were performed,the tear film rupture time was recorded,and the levels of IL-18 and TSLP in tear were detected by enzyme-linked immunosorbent assay.Results:There were no significant differences in SIT,BUT,FL score,tear IL-18,TSLP levels on the 1st day of the experiment between the two groups(all P>0.05).The SIT and BUT values on the 15th day of the experiment in the model group were lower than those of the control group,and also less than those on the 1st day of the experiment(all P<0.05).The FL score,IL-18 and TSLP levels of the model group on the 15th day of the experiment were higher than those of the control group,and also higher than those on the 1st day of the experiment(all P<0.05).Conclusion:TSLP is highly expressed in the tears of mice with dry eye,which may inhibit the release of neurotransmitters,promote the expression of IL-18 in tears,and reduce secretion of tears,so as to induce the occurrence of dry eye.

参考文献/References:

[1] 崔 红,李春华,李正日,等.非肥胖性糖尿病小鼠干眼模型的初步建立[J].国际眼科杂志,2019,19(8):1293-1296.
[2] 王小东,李宏松,程 钰,等.三种角膜修复剂促角膜损伤修复的临床效果观察[J].陕西医学杂志,2017,46(2):218-220.
[3] Nakajima S,Kabata H,Kabashima K,et al.Anti-TSLP antibodies:Targeting a master regulator of type 2 immune responses[J].Allergol Int,2020,69(2):197-203.
[4] Ogasawara N,Poposki JA,Klingler AI,et al.Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps[J].Immun Inflamm Dis,2020,13(1):86-95.
[5] Steinmetz M,Laurans L,Nordsiek S,et al.Thymic stromal lymphopoietin is a key cytokine for the immunomodulation of atherogenesis with Freund's adjuvant[J].J Cell Mol Med,2020,24(10):5731-5739.
[6] 任娜欣.芪明颗粒联合氟米龙滴眼液治疗儿童中重度干眼症的疗效观察[J].陕西中医,2018,39(1):26-28.
[7] 王 茜,万 磊,李 晶,等.糖尿病小鼠相关干眼眼表组织动态变化特征[J].中华实验眼科杂志,2019,37(6):419-424.
[8] 杜 婧,李 勇,高金荣,等.白介素1受体相关激酶1(IRAK1)和NF-κB在苯扎氯铵诱导的干眼症小鼠角膜和结膜组织中的表达[J].眼科新进展,2019,39(3):223-228.
[9] 张立颖,褚俏梅,李方都.Graves眼病患者经131I治疗后血清IL-17、IGF-1、ICAM-1检测分析及临床意义[J].陕西医学杂志,2019,48(3):393-396.
[10] 王彦方.碱性成纤维细胞生长因子对干眼小鼠模型眼表白细胞介素-10和白细胞介素-1β蛋白表达的影响[J].中国临床药理学杂志,2018,34(14):1671-1674.
[11] 杨雁嫦,林 启,袁 晴,等.H2滴眼液对苯扎溴铵导致的小鼠眼表损伤的预防作用[J].眼科新进展,2019,39(1):8-11.
[12] 李 娟,周 颖,谭 钢,等.间充质干细胞外泌体治疗小鼠干眼的疗效评价[J].眼科新进展,2019,39(10):901-905.
[13] 胡晓琴,张梦瑶,朱佩文,等.富血小板血浆(PRP)裂解液在苯扎溴胺诱导的小鼠干眼治疗中的作用[J].眼科新进展,2019,39(11):1019-1023.
[14] Truffault F,Nazzal D,Verdier J,et al.Comparative analysis of thymic and blood treg in myasthenia gravis:Thymic epithelial cells contribute to thymic immunoregulatory defects[J].Front Immunol,2020,11:782.
[15] 闵幼兰,刘佳祥,贺 赢,等.西酞普兰对小鼠角膜上皮细胞和泪膜功能的影响[J].中国现代医学杂志,2019,29(1):1-6.
[16] Cvitas I,Galichet A,Ling SC,et al.Toll-like receptor-ligand induced thymic stromal lymphopoietin expression in primary equine keratinocytes[J].Veterinary Dermatology,2020,31(2):154-156.
[17] Guo H,Ji X,Yang G,et al.Abnormal thymic stromal lymphopoietin expression in the gastrointestinal mucosa of patients with eosinophilic gastroenteritis[J].J Pediatr(Rio J),2020,96(3):350-355.
[18] Lee HY,Hur J.Blockade of thymic stromal lymphopoietin and CRTH2 attenuates airway inflammation in a murine model of allergic asthma[J].Vet Dermatol,2020,35(3):619-629.
[19] Li F,Dong Y,Ni C,et al.Fine particulate matter(PM2.5)is a risk factor for dermatitis by promoting the expression of thymic stromal lymphopoietin(TSLP)in keratinocytes[J].Indian J Dermatol,2020,65(2):92-96.
[20] Lorz LR,Kim MY,Cho JY.Medicinal potential of panax ginseng and its ginsenosides in atopic dermatitis treatment[J].J Ginseng Res,2020,44(1):8-13.

相似文献/References:

[1]陈 润,孙丽丽,冯晓芳,等.有晶状体眼后房型人工晶状体植入术对患者眼表泪膜影响临床研究[J].陕西医学杂志,2024,(10):1371.[doi:DOI:10.3969/j.issn.1000-7377.2024.10.016]
　CHEN Run,SUN Lili,FENG Xiaofang,et al.Effect of implantable collamer lens implantation on ocular surface tear film[J].,2024,(11):1371.[doi:DOI:10.3969/j.issn.1000-7377.2024.10.016]

备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2019YFF0216502)
更新日期/Last Update: 2021-11-05