[1]石晓岚,赵 龙,王 宁,等.miR-542-3p对脂多糖诱导的人支气管上皮细胞增殖和迁移的影响及机制研究[J].陕西医学杂志,2021,50(10):1218-1222.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.010]
　SHI Xiaolan,ZHAO Long,WANG Ning,et al.Effect and mechanism of miR-542-3p on the proliferation and migration of LPS-induced human bronchial epithelial cells[J].,2021,50(10):1218-1222.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.010]
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miR-542-3p对脂多糖诱导的人支气管上皮细胞增殖和迁移的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年10期
页码:
1218-1222
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
Effect and mechanism of miR-542-3p on the proliferation and migration of LPS-induced human bronchial epithelial cells
作者:
石晓岚; 赵 龙; 王 宁; 刘翠翠; 王 静; 马彩铃
(西安市儿童医院呼吸哮喘中心,陕西 西安710002)
Author(s):
SHI Xiaolan; ZHAO Long; WANG Ning; LIU Cuicui; WANG Jing; MA Cailing
(Respiratory Asthma Center,Xi'an Children's Hospital,Xi'an 710002,China)
关键词:
miR-542-3p;  IL-33/sST2信号通路;  儿童呼吸哮喘;  细胞增殖;  细胞迁移
Keywords:
MiR-542-3p;  IL-33/sST2 signaling pathway;  Childhood respiratory asthma;  Cell proliferation;  Cell migration
分类号:
R 392.12
DOI:
DOI:10.3969/j.issn.1000-7377.2021.10.010
文献标志码:
A
摘要:
目的:探讨miR-542-3p在儿童呼吸哮喘中的作用及其可能机制。方法:用不同浓度的脂多糖(LPS)分别处理BEAS-2B细胞24 h构建哮喘细胞模型,采用脂质体法将miR-542-3p模拟物、抑制物及对照转染至BEAS-2B。RT-qPCR检测miR-542-3p的表达水平,CCK-8和划痕法检测细胞增殖和迁移能力。RT-qPCR和western blot法检测白细胞介素33(IL-33)和可溶性生长刺激表达基因2蛋白(sST2)的mRNA和蛋白表达水平的变化。通过在线网站microRNA.org预测分析miR-542-3p与IL-33的靶向关系,使用双萤光素酶报告基因实验进行验证,并对miR-542-3p与IL-33进行Spearman相关性分析。通过共转染miR-542-3p和pcDNA3.1-IL-33,检测IL-33对miR-542-3p功能的影响。结果:与未处理组(untreated)相比,miR-542-3p在LPS处理的BEAS-2B细胞中相对表达量显著下调(P<0.05),而IL-33和sST2的mRNA相对表达量均显著上调(均P<0.05)。miR-542-3p下调IL-33的表达(P<0.05),过表达IL-33抑制miR-542-3p对LPS诱导的BEAS-2B增殖和迁移的影响(均P<0.05)。结论:miR-542-3p可能通过抑制IL-33/sST2信号通路缓解儿童呼吸哮喘。
Abstract:
Objective:To investigate the effect of miR-542-3p on childhood respiratory asthma and its mechanism.Methods:BEAS-2B cells were treated with different concentrations of Lipopolysaccharide(LPS)for 24 hours to construct asthma cell model.The miR-542-3p mimic,inhibitor as well as their respective controls were transfected into BEAS-2B via liposome method.RT-qPCR was used to detect the expression level of miR-542-3p.CCK-8 and wound-healing assay were used to determine the effect of miR-542-3p on the cell proliferation and migration.The mRNA and protein expression levels of IL-33 and sST2 were detected by RT-qPCR and Western blot.The relationship between miR-542-3p and IL-33 was predicted by microRNA.org and verified by double-luciferase reporter gene experiment.Spearman correlation analysis was performed to analyze the correlation between miR-542-3p and IL-33.The miR-542-3p and pcDNA3.1-IL-33 were co-transfected to detect the effect of IL-33 on the function of miR-542-3p.Results:Compared with untreated group,the relative mRNA expression of miR-542-3p was significantly inhibited while IL-33 and sST2 were up-regulated in LPS-stimulated BEAS-2B(all P<0.05).The miR-542-3p down-regulated the expression of IL-33,while overexpressing IL-33 inhibited the effect of miR-542-3p on LPS-induced BEAS-2B proliferation and migration(P<0.05).Conclusion:In LPS-induced BEAS-2B,miR-542-3p may alleviate childhood respiratory asthma by inhibiting IL-33/sST2 signaling pathway.

参考文献/References:

[1] 王艺红.哮喘干预始于孕前[J].江苏卫生保健,2015,16(19):43.
[2] Ameis D,Khoshgoo N,Iwasiow BM,et al. MicroRNAs in lung development and disease[J].Paediatric Respiratory Reviews,2017,22:38-43.
[3] Shi ZG,Sun Y,Wang KS,et al. Effects of miR-26a/miR-146a/miR-31 on airway inflammation of asthma mice and asthma children[J].European Review for Medical and Pharmacological Sciences,2019,23(12):5432.
[4] Hannafon BN,Cai A,Calloway CL,et al. miR-23b and miR-27b are oncogenic microRNAs in breast cancer:Evidence from a CRISPR/Cas9 deletion study[J].BMC Cancer,2019,19(1):1-12.
[5] 全细云,李 明,陈栋良,等.胃癌组织中microRNA-214和MGAT5表达及预后的相关性分析[J].陕西医学杂志,2019,48(1):117-120.
[6] 韩 乐,陈文娟,雷光焰,等.microRNA-21、microRNA-25在肺癌组织中的表达及意义[J].陕西医学杂志,2019,48(12):1596-1600.
[7] 赵 垚.miR-506和miR-542-3p在恶变的支气管上皮细胞中的功能[D].广州:广州医学院,2011.
[8] 李芙蓉,王捷军.儿童支气管哮喘控制不良因素中鼻窦炎患病率观察[J].中国实用内科杂志,2014,25(5):106-108.
[9] 林 惠,颜 芬.微小RNA-195-5p与脑源性神经营养因子在哮喘急性发作期患儿血清中表达相关性研究[J].陕西医学杂志,2020,49(7):862-865.
[10] 邢西迁,杨 姣,白 敏,等.哮喘miRNA-mRNA调节网络的构建与小分子药物的预测[J].中国临床药理学杂志,2021,37(4):454-457.
[11] Liu B,Li J,Zheng M,et al. MiR-542-3p exerts tumor suppressive functions in non-small cell lung cancer cells by upregulating FTSJ2[J].Life Sciences,2017,188:87-95.
[12] Tao J,Liu Z,Wang Y,et al. MiR-542-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting UBE3C[J].Biomedicine & Pharmacotherapy,2017,93:420-428.
[13] Li J,Shao W,Feng H.MiR-542-3p,a microRNA targeting CDK14,suppresses cell proliferation,invasiveness,and tumorigenesis of epithelial ovarian cancer[J].Biomedicine & Pharmacotherapy,2019,110:850-856.
[14] 赵 垚,刘焕英,李远奇,等. miR-542-3p在反式-7,8-二羟-9,10-环氧苯并芘诱导致癌中的作用[J].中华预防医学杂志,2011,45(5):416-421.
[15] 王 跃,郑志超,谷小虎,等.miRNA-542-3p对胃腺癌细胞侵袭转移能力的影响[J].解剖科学进展,2018,24(1):72-75.
[16] 李沛隆,连 杰,林 娜,等.白细胞介素-33与儿童支气管哮喘的相关性研究进展[J].新乡医学院学报,2021,38(4):374-377.
[17] 李 鑫 刘圆圆,张才擎.白介素-33在支气管哮喘发病机制中的作用[J].中华临床医师杂志:电子版,2019,13(5):389-392.
[18] Rankin AL,Mumm JB,Murphy E,et al. IL-33 induces IL-13-Dependent cutaneous fibrosis[J].Journal of Immunology,2010,184(3):1526-1535.
[19] Tajima S,Oshikawa K,Tominaga S,et al. The increase in serum soluble ST2 protein upon acute exacerbation of idiopathic pulmonary fibrosis[J].Chest,2003,124(4):1206-1214.

备注/Memo

备注/Memo:
基金项目:西安市儿童医院院级科研项目(2018C04)
更新日期/Last Update: 2021-10-08