[1]李 新,白 雪,李 娜.阿霉素诱导心脏损伤模型大鼠超声心功能指标与核因子κB p65表达水平关系[J].陕西医学杂志,2021,50(10):1187-1190.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.003]
　LI Xin,BAI Xue,LI Na.Relationship between echocardiac function indexes and the expression level of NF-κB p65 in model rats with heart injury induced by adriamycin[J].,2021,50(10):1187-1190.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.003]
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阿霉素诱导心脏损伤模型大鼠超声心功能指标与核因子κB p65表达水平关系
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年10期
页码:
1187-1190
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
Relationship between echocardiac function indexes and the expression level of NF-κB p65 in model rats with heart injury induced by adriamycin
作者:
李 新1; 白 雪2; 李 娜3
(1.铜川市人民医院超声医学科, 陕西 铜川 727000; 2.安康市中心医院超声科,陕西 安康725000; 3.西安交通大学第一附属医院检验科,陕西 西安 710061)
Author(s):
LI Xin; BAI Xue; LI Na
(Department of Ultrasound Medicine,Tongchuan People's Hospital,Tongchuan 727000,China)
关键词:
心脏损伤;  阿霉素;  超声心功能;  核因子κB;  超氧化物歧化酶;  相关性;  大鼠
Keywords:
Heart injury;  Adriamycin;  Echocardiac function;  Nuclear factor κB;  Superoxide dismutase;  Correlation;  Rats
分类号:
R 332
DOI:
DOI:10.3969/j.issn.1000-7377.2021.10.003
文献标志码:
A
摘要:
目的:探讨阿霉素诱导心脏损伤模型大鼠的超声心功能指标与核因子κB(NF-κB)p65表达水平的关系。方法:清洁级雄性健康成年Sprague-Dawley(SD)大鼠72只随机平分为三组:对照组、模型A组、模型B组,三组分别给予腹腔注射0、1、5 ml/kg阿霉素,在造模第1周与第2周每组各处死12只大鼠,检测大鼠的超声心功能指标与NF-κB p65表达水平。结果:模型A组、模型B组造模第1周与第2周的左室收缩压(LVSP)、左室舒张末压(LVEDP)、测丙二醛(MDA)高于对照组(均P<0.05),左室射血分数(LVEF)、左室短轴缩短率(LVFS)、超氧化物歧化酶(SOD)、NF-κB p65低于对照组(均P<0.05),模型A组与模型B组比较差异均有统计学意义(P<0.05)。在模型B组中,造模第2周的NF-κB p65蛋白相对表达水平与LVSP、LVEDP、LVEF、LVFS都存在相关性(均P<0.05)。结论:阿霉素诱导心脏损伤模型大鼠多伴随有超声心功能指标的异常与NF-κB p65表达水平下降,两者存在相关性。
Abstract:
Objective:To investigate the relationship between the echocardial function indexes and the expression of nuclear factor kappa-B(NF-κB)p65 in model rats with heart injury induced by adriamycin.Methods:72 clean-grade male healthy adult Sprague-Dawley(SD)rats were randomly divided into control group,model 1 group,and model 2 group.The three groups were given intraperitoneal injections of 0,1and 5 ml/ kg of adriamycin respectively.There were 12 rats in each group died at the 1st and 2nd week of modeling,and the echocardiographic function indexes and NF-κB p65 expression level of the model rats were detected.Results:The left ventricular systolic blood pressure(LVSP),left ventricular end diastolic pressure(LVEDP),and malondialdehyde(MDA)at the 1st and 2nd week of modeling in the model 1 group and model 2 group were higher than those in the control group(all P<0.05),left ventricular ejection fraction(LVEF),left ventricular short axis shortening rate(LVFS),superoxide dismutase(SOD)and NF-κB p65 were lower than those of the control group(all P<0.05),the difference between the model 1 group and model 2 group were also statistically significant(all P<0.05).In the model 2 group,the relative expression level of NF-κB p65 protein at the 2nd week of modeling were correlated with LVSP,LVEDP,LVEF and LVFS(all P<0.05).Conclusion:Adriamycin-induced heart injury model rats are mostly accompanied by abnormal echocardiographic indexes and decreased expression of NF-κB p65,and there are correlation between NF-κB p65 and echocardiographic indexes.

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备注/Memo

备注/Memo:
基金项目:陕西省缺血性心血管疾病重点实验室项目(18JS103)
更新日期/Last Update: 2021-10-08