[1]傅 娟,刘媛媛,李 霞.穗花杉双黄酮抑制小儿血管瘤内皮细胞体外增殖实验研究[J].陕西医学杂志,2021,50(8):937-940,965.[doi:DOI:10.3969/j.issn.1000-7377.2021.08.009]
　FU Juan,LIU Yuanyuan,LI Xia.Inhibitory effect of Amentoflame on proliferation of endothelial cells from infantile hemangioma in vitro[J].,2021,50(8):937-940,965.[doi:DOI:10.3969/j.issn.1000-7377.2021.08.009]
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穗花杉双黄酮抑制小儿血管瘤内皮细胞体外增殖实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年8期
页码:
937-940,965
栏目:
基础研究
出版日期:
2021-08-05

文章信息/Info

Title:
Inhibitory effect of Amentoflame on proliferation of endothelial cells from infantile hemangioma in vitro
作者:
傅 娟1; 刘媛媛2; 李 霞3
(1.固原市人民医院,宁夏 固原 756000; 2.合阳县人民医院,陕西 合阳 715316; 3.西安高新医院,陕西 西安 710075)
Author(s):
FU Juan; LIU Yuanyuan; LI Xia
(Guyuan People's Hospital,Guyuan 756000,China)
关键词:
小儿血管瘤内皮细胞;  穗花杉双黄酮;  增殖;  细胞周期;  细胞活力;  细胞周期蛋白D1
Keywords:
Endothelial cells from infantile hemangioma;  Amentoflame;  Proliferation;  Cell cycle;  Cell vitality;  Cyclin D1
分类号:
R 732.2
DOI:
DOI:10.3969/j.issn.1000-7377.2021.08.009
文献标志码:
A
摘要:
目的:探究穗花杉双黄酮(AF)对小儿血管瘤内皮细胞体外增殖的影响。方法:MTS法筛选AF抑制小儿血管瘤内皮细胞体外增殖的适宜浓度,流式细胞法检测AF对小儿血管瘤内皮细胞细胞周期G1期和S期分布的影响,荧光定量PCR和Western blot法检测AF对小儿血管瘤内皮细胞增殖相关蛋白表达的影响。结果:75、125 μmol/L AF显著降低小儿血管瘤内皮细胞活性(P<0.05),75 μmol/L AF用于后续细胞周期实验、荧光定量PCR和Western blot实验,结果显示75 μmol/L AF抑制小儿血管瘤内皮细胞从G1期进入S期(P<0.05),但对G2其细胞分布无明显作用,与对照组相比较,AF显著抑制小儿血管瘤内皮细胞β-catenin和Cyclin D1 mRNA 和蛋白表达,促进p21 mRNA和蛋白表达(均P<0.05)。结论:AF通过抑制β-catenin和Cyclin D1,促进p21等增殖相关蛋白表达进而抑制小儿血管瘤内皮细胞体外增殖。
Abstract:
Objective:To explore the effect of Amentoflame(AF)on the proliferation of endothelial cells from infantile hemangioma in vitro.Methods:MTS method was used to screen the appropriate concentration of AF to inhibit the proliferation of endothelial cells from infantile hemangioma in vitro.Flow cytometry was used to detect the effect of AF on the distribution of endothelial cells from infantile hemangioma in the G1 and S phases.Fluorescence quantitative PCR and Western blot were used to detect the effect of AF on the expression of proliferation-related proteins in the endothelial cells from infantile hemangioma.Results:75 and 125 μmol/L AF significantly reduced the activity of endothelial cells from infantile hemangioma.75 μmol/L AF was used in subsequent cell cycle experiments,fluorescent quantitative PCR and Western blot experiments.The results indicated that 75 μmol/L AF inhibited the endothelial cells from infantile hemangioma to enter the S phase from G1 phase,but had no obvious effect on the cell distribution of G2 phase.Compared with the control group,AF significantly inhibited the expression of β-catenin and Cyclin D1 mRNA and protein in endothelial cells from infantile hemangioma,and promoted the expression of p21 mRNA and protein(all P<0.05).Conclusion:AF can promote the expression of proliferation-related proteins such as p21 by inhibiting β-catenin and Cyclin D1,and thereby can inhibit the proliferation of endothelial cells from infantile hemangioma in vitro.

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更新日期/Last Update: 2021-08-05