[1]王 月,刘佳俊,王 雷,等.缺血后处理通过升高血清腺苷水平减轻脑缺血再灌注损伤的实验研究[J].陕西医学杂志,2021,50(7):789-792.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.005]
　WANG Yue,LIU Jiajun,WANG Lei,et al.Experimental study of ischemic postconditioning on reducing cerebral ischemia-reperfusion injury by increasing adenosine[J].,2021,50(7):789-792.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.005]
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缺血后处理通过升高血清腺苷水平减轻脑缺血再灌注损伤的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年7期
页码:
789-792
栏目:
基础研究
出版日期:
2021-07-05

文章信息/Info

Title:
Experimental study of ischemic postconditioning on reducing cerebral ischemia-reperfusion injury by increasing adenosine
作者:
王 月1; 刘佳俊2; 王 雷2; 王 峰2
(1.锦州医科大学齐齐哈尔医学院附属第一医院研究生培养基地,黑龙江 齐齐哈尔161041; 2.齐齐哈尔医学院附属第一医院,黑龙江 齐齐哈尔161041)
Author(s):
WANG Yue; LIU Jiajun; WANG Lei; WANG Feng
(Graduate Training Base,First Affiliated Hospital of Qiqihar Medical College,Jinzhou Medical University,Qiqihar 161041,China)
关键词:
脑缺血再灌注损伤;  缺血后处理;  腺苷受体;  相关性;  脑组织;  神经元
Keywords:
Cerebral ischemia-reperfusion injury;  Ischemic postconditioning;  Adenosine receptor;  Correlation;  Brain tissue;  Neuron
分类号:
R 743.31
DOI:
DOI:10.3969/j.issn.1000-7377.2021.07.005
文献标志码:
A
摘要:
目的:研究缺血后处理与脑缺血再灌注损伤中腺苷受体水平的关系。方法:取健康雄性大鼠(SD)48只,将其按照随机数字表法分作假手术(Sham组),I/R组(缺血再灌注组),腺苷受体拮抗剂组各6只,剩余30只为I/R+缺血再灌注远端缺血后处理组(RIPoC组)。其中I/R+RIPoC组又分为5个亚组,分别为Ⅰ-Ⅴ组,每组各6只。Sham组单纯暴露第一颈椎横突翼状孔与颈总动脉。I/R组实施8 min脑缺血处理,且待再灌注后关闭伤口。I/R+RIPoC组则于再灌注开始后0 h、开始后2、3、4.5、6 h夹闭双侧股动脉实行15 min缺血/15 min再灌注,共计3个循环。分析各组大鼠脑组织神经元水平,血清腺苷水平的差异。脑缺血再灌注损伤大鼠神经元和影响因素的关系实施多因素Logistic回归分析。结果:I/R组、腺苷受体拮抗组大鼠神经元均明显低于Sham组,且I/R+RIPoC组大鼠神经元均高于I/R组,其中Ⅰ-Ⅴ组大鼠神经元呈逐渐降低趋势,且各组之间对比差异均有统计学意义; 同时,腺苷受体拮抗组大鼠神经元明显低于I/R组(均P<0.05)。I/R组大鼠血清腺苷水平明显高于Sham组,且I/R+RIPoC组大鼠血清腺苷水平均高于I/R组(均P<0.05)。经多因素Logistic回归分析发现:缺血后处理、血清腺苷均是脑缺血再灌注损伤大鼠神经元的保护性因素(OR<1,均P<0.05)。结论:缺血后处理对脑缺血再灌注损伤具有一定的保护作用,且该保护作用和血清腺苷水平存在密切相关。
Abstract:
Objective:To study the relationship between ischemic postconditioning and adenosine level in cerebral ischemia-reperfusion injury.Methods:48 Healthy male SD rats were selected and divided into sham operation(Sham)group(6 rats),I/R group(ischemia reperfusion group,6 rats),adenosine receptor antagonist group(6 rats),and I/R+RIPoC group(remote ischemia-reperfusion ischemia postconditioning group,30 rats).The I/R+RIPoC group was divided into 5 subgroups,namely groups Ⅰ-Ⅴ,with 6 rats in each subgroup.In the Sham group,the first cervical transverse process pterygoid foramen and the common carotid artery were exposed.The I/R group was treated with cerebral ischemia for 8 minutes,and the wound was closed after reperfusion.In the I/R + RIPoC group,the bilateral femoral arteries were clamped at 0,2,3,4.5,and 6 hours after the start of reperfusion,and 15 minutes ischemia/15 minutes reperfusion was performed,for a total of 3 cycles.The differences in the levels of neurons and serum adenosine in the brain tissues of rats in each group were analyzed.Multivariate Logistic regression was used to analyze the influencing factors of cerebral ischemia-reperfusion injury in rats' neurons.Results:The neurons in the I/R group and the adenosine receptor antagonist group were significantly lower than those in the Sham group.The neurons in the I/R+RIPoC group were higher than those in the I/R group,among which the rats' neurons in groups Ⅰ-Ⅴ showed a gradual decrease trend,and the differences between the groups were statistically significant(all P<0.05).At the same time,the neurons in the adenosine receptor antagonist group were significantly lower than that in the I/R group(P<0.05).The level of serum adenosine in I/R group was significantly higher than that in Sham group,and the level of serum adenosine in I/R+RIPoC group was higher than that in I/R group(all P<0.05).Multivariate Logistic regression analysis found that ischemic postconditioning and serum adenosine were both protective factors for neurons in rats with cerebral ischemia-reperfusion injury(all OR<1,all P<0.05).Conclusion:Ischemic postconditioning has a certain protective effect on cerebral ischemia-reperfusion injury,and this protective effect is closely related to serum adenosine level.

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备注/Memo

备注/Memo:
基金项目:黑龙江省卫生健康委员会科研课题(2017-267)
更新日期/Last Update: 2021-07-05