[1]何 瑜,唐宽平,马晓颖.5-钙黏蛋白、基质金属蛋白酶9在结直肠癌组织中的表达及临床意义[J].陕西医学杂志,2021,50(5):618-621.[doi:DOI:10.3969/j.issn.1000-7377.2021.05.026]
　HE Yu,TANG Kuanping,MA Xiaoying.Expression and clinical significance of E-cadherin and matrix metalloproteinase 9 in colorectal cancer[J].,2021,50(5):618-621.[doi:DOI:10.3969/j.issn.1000-7377.2021.05.026]
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5-钙黏蛋白、基质金属蛋白酶9在结直肠癌组织中的表达及临床意义

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年5期
页码:
618-621
栏目:
临床病理
出版日期:
2021-05-05

文章信息/Info

Title:
Expression and clinical significance of E-cadherin and matrix metalloproteinase 9 in colorectal cancer
作者:
何 瑜1; 唐宽平2; 马晓颖1
(1.陕西省人民医院,陕西 西安 710068; 2.解放军总医院京中医疗区旃坛寺门诊部,北京 100034)
Author(s):
HE Yu; TANG Kuanping; MA Xiaoying
(Shaanxi Provincial People's Hospital,Xi'an 710068,China)
关键词:
E-钙黏蛋白;  基质金属蛋白酶9;  结直肠癌;  复发;  预测
Keywords:
E-cadherin;  Matrix metalloproteinase 9;  Colorectal cancer;  Recurrence;  Predict
分类号:
R 735.3
DOI:
DOI:10.3969/j.issn.1000-7377.2021.05.026
文献标志码:
A
摘要:
目的:探讨E-钙黏蛋白(E-cadherin)、基质金属蛋白酶9(MMP-9)在结直肠癌组织中的表达及临床意义。方法:采用免疫组化法检测86例结直肠癌患者癌组织和对应癌旁组织中E-cadherin、MMP-9蛋白积分光密度(IOD)值。随访1年,统计结直肠癌患者复发情况,比较复发者与未复发者临床特征,采用Logistic多因素回归分析法分析影响结直肠癌患者术后复发的因素,采用受试者工作特征曲线(ROC)分析结直肠癌组织中E-cadherin、MMP-9蛋白IOD值预测术后复发的效能。结果:癌组织中E-cadherin蛋白IOD值低于癌旁组织(P<0.05),MMP-9蛋白IOD值高于癌旁组织(P<0.05); 随访1年,结直肠癌患者复发率为25.30%; 复发者TNM分期、术前淋巴结转移构成比以及E-cadherin蛋白IOD值、MMP-9蛋白IOD值与未复发者比较,差异均有统计学意义(均P<0.05); Logistic多因素回归分析显示E-cadherin蛋白IOD值、MMP-9蛋白IOD值、术前淋巴结转移均是结直肠癌患者术后复发的独立危险因素(OR=3.350、3.117、2.664,均P<0.05); ROC分析显示,癌组织中E-cadherin、MMP-9蛋白IOD值预测结直肠癌患者术后复发的最佳截断点分别为0.68和10.29,灵敏度分别为80.95%和85.71%,特异度分别为87.10%和80.65%,曲线下面积(AUC)分别为0.865和0.905。结论:结直肠癌患者癌组织E-cadherin蛋白低表达,MMP-9蛋白高表达,且E-cadherin、MMP-9蛋白表达水平均是结直肠癌患者术后复发的独立危险因素,两者均可作为预测结直肠癌患者术后复发的敏感指标。
Abstract:
Objective:To explore the expression and clinical significance of E-cadherin and matrix metalloproteinase 9(MMP-9)in colorectal cancer.Methods:Eighty-six patients with colorectal cancer were selected,and the immunohistochemical method was used to detect the expression of E-cadherin and MMP-9 protein in cancer tissues and corresponding adjacent tissues,and the integrated optical density(IOD)was determined.Followed-up for 1 year,the recurrence of colorectal cancer patients were counted,the clinical characteristics of relapsed and non-recurring patients were compared.Logistic multivariate factor regression analysis was used to analyze the factors affecting postoperative recurrence of colorectal cancer patients.The receiver operating characteristic(ROC)curve was used to analyze the efficacy of IOD values of E-cadherin and MMP-9 protein in colorectal cancer tissues in predicting postoperative recurrence.Results:The IOD value of E-cadherin protein in cancer tissue was lower than that in adjacent tissues,and the IOD value of MMP-9 protein was higher than that in adjacent tissues(all P<0.05).After 1 year of follow-up,the recurrence rate of colorectal cancer patients was 25.30%,the TNM staging and lymph node metastasis composition ratio before surgery and E-cadherin protein IOD value,MMP-9 protein IOD value of relapsed patients were statistically different from non-relapsed patients(P<0.05).Logistic multivariate regression analysis showed that E-cadherin protein IOD value,MMP-9 protein IOD value,and lymph node metastasis before surgery were independent risk factors for postoperative recurrence of colorectal cancer patients(OR=3.350,3.117,2.664,all P<0.05).ROC analysis showed that the best cut-off points of IOD values of E-cadherin and MMP-9 protein in cancer tissues for predicting postoperative recurrence of colorectal cancer patients were respectively 0.68 and 10.29,the sensitivity were 80.95% and 85.71%,and the specificity were 87.10% and 80.65%,and the area under the curve(AUC)were 0.865 and 0.905.Conclusion:The expression of E-cadherin protein in cancer tissues of patients with colorectal cancer is decreased,while the expression of MMP-9 protein is increased.E-cadherin and MMP-9 protein expression levels are independent risk factors for postoperative recurrence in patients with colorectal cancer,which can be used as predictors of colorectal cancer sensitive indicators of postoperative recurrence.

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更新日期/Last Update: 2021-05-06