[1]唐一萍,冯 俊,马 鹏,等.miRNA-183在噪声性耳聋大鼠耳蜗组织中的表达及临床意义[J].陕西医学杂志,2021,50(5):538-541.[doi:DOI:10.3969/j.issn.1000-7377.2021.05.006]
　TANG Yiping,FENG Jun,MA Peng,et al.Expression of miRNA-183 in cochlea tissue of noise-deaf rats and its clinical significance[J].,2021,50(5):538-541.[doi:DOI:10.3969/j.issn.1000-7377.2021.05.006]
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miRNA-183在噪声性耳聋大鼠耳蜗组织中的表达及临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年5期
页码:
538-541
栏目:
基础研究
出版日期:
2021-05-05

文章信息/Info

Title:
Expression of miRNA-183 in cochlea tissue of noise-deaf rats and its clinical significance
作者:
唐一萍; 冯 俊; 马 鹏; 罗德艳
(南充市中心医院 川北医学院第二临床医学院耳鼻咽喉头颈外科,四川 南充637000)
Author(s):
TANG Yiping; FENG Jun; MA Peng; LUO Deyan
(Department of Otorhinolaryngology-Head and Neck Surgery,Nanchong Central Hospital,Nanchong 637000,China)
关键词:
噪声性耳聋;  miRNA-183;  大鼠;  耳蜗组织;  毛细胞
Keywords:
Noise-induced hearing loss;  miRNA-183;  Rats;  Cochlear tissue;  Hair cell
分类号:
R 764.43
DOI:
DOI:10.3969/j.issn.1000-7377.2021.05.006
文献标志码:
A
摘要:
目的:探究噪声性耳聋大鼠耳蜗组织中miRNA-183表达情况,并分析其表达水平变化对噪声性耳聋发生发展的影响。方法:将50只SD大鼠分为两组,其中10只设为空白对照组,另40只建立噪声性耳聋动物模型,并随机分为噪声暴露1 d组(n=10)、噪声暴露7 d组(n=10)、噪声暴露14 d组(n=10)、噪声暴露28 d组(n=10)。处死各组大鼠取出耳蜗组织,应用生物显微镜观察耳蜗基底膜内外毛细胞排列情况,并应用RT-PCR法检测miRNA-183表达水平,比较各组大鼠耳蜗基底膜镜检结果及miRNA-183表达差异。结果:在生物显微镜下可见,空白对照组大鼠耳蜗基底膜的內毛、外毛细胞列整齐,而噪声暴露1 d组、噪声暴露7 d组、噪声暴露14 d组、噪声暴露28 d组的外毛细胞排列均紊乱。Tamhane事后检验显示,外毛细胞缺失率随噪声暴露时间延长而显著升高(P<0.05)。与空白对照组相比,噪声暴露1 d组、噪声暴露7 d组、噪声暴露14 d组、噪声暴露28 d组的miRNA-183相对表达量均显著降低(均P<0.05)。结论:miRNA-183在噪声性耳聋大鼠耳蜗组织中呈低表达,miRNA-183表达量下调可能会促进噪声性耳聋发生发展,而且对耳蜗毛细胞损伤修复评估具有一定的临床意义。
Abstract:
Objective:To investigate the expression of miRNA-183 in the cochlea tissue of noise-induced deafness rats,and analyze the influence of its expression level on the development of noise-induced deafness.Methods:Fifty SD rats were divided into two groups,of which 10 SD rats were set as blank control group,and another 40 SD rats were established as animal models of noise-induced deafness,and randomly divided into noise exposure 1 day group(n=10),Noise exposure 7 days group(n=10),noise exposure 14 days group(n=10),noise exposure 28 days group(n=10).The rats in each group were sacrificed and the cochlear tissues were taken out.The arrangement of the inner and outer hair cells of the cochlear basement membrane was observed with biological microscope,and the expression level of miRNA-183 was detected by RT-PCR.The results of the cochlear basement membrane microscopy and miRNA-183 expression of each group were compared.Results:It can be seen under the biological microscope that the inner and outer hair cells of the cochlea basement membrane of the blank control group were neatly arranged,while the outer hair cells of the other four groups were arranged disorderly.The loss rate of outer hair increased significantly with prolonged noise exposure time(P<0.05).Compared with the blank control group,the relative expression of miRNA-183 in the other four groups was significantly reduced(all P<0.05).Conclusion:The expression of miRNA-183 is low in the cochlear tissue of noise-deaf rats.Down-regulation of miRNA-183 expression may promote the occurrence and development of noise-deaf,and has certain clinical significance for the evaluation of cochlear hair cell damage repair.

参考文献/References:

[1] 余善法,郑玉新.探索噪声性听力损失精准预防之路[J].中华预防医学杂志,2017,51(1):5-7.
[2] 孙 青,秦雪梅,曹德林,等.促红细胞生成素对老年性耳聋小鼠血清和耳蜗组织中IL-6和IL-17表达的影响[J].陕西医学杂志,2020,49(5):531-533,537.
[3] 蔡朝阳,江 天.突发性耳聋的基因学研究进展[J].浙江临床医学,2018,20(7):1312-1314.
[4] Ding L,Liu J,Shen HX,et al.Analysis of plasma microRNA expression profiles in male textile workers with noise-induced hearing loss[J].Hear Res,2016,333:275-282.
[5] 于碧鲲,崔 鹂,夏 源,等.噪声性听力损失大鼠耳蜗miRNA调控网络分析[J].现代预防医学,2016,43(1):124-127,135.
[6] 何 珍,张 滟,张 晶,等.慢性中耳炎患者鼓室成形术后耳鸣,听力变化及影响耳鸣相关因素分析[J].陕西医学杂志,2018,47(12):1588-1594.
[7] 李江丽,刘积平,文玉敏,等.畸变产物耳声发射在噪声暴露听力正常检测中的临床价值[J].右江医学,2019,47(2):92-94.
[8] Blioskas S,Tsalighopoulos M,Psillas G,et al.Utility of otoacoustic emissions and olivocochlear reflex in predicting vulnerability to noise-induced inner ear damage[J].Noise Health,2018,20(94):101-111.
[9] 田永远,吴学志,张安东,等.清肝通窍汤治疗肝火上扰型暴聋患者临床研究[J].陕西中医,2018,39(11):1548-1550,1568.180.
[10] 陈 秉,夏 源,王海涛,等.噪声性听力损失与耳蜗外毛细胞Prestin蛋白的相关性分析[J].中国工业医学杂志,2015,28(2):92-94.
[11] Peyvandi AA,Abbaszadeh HA,Roozbahany NA,et al.Deferoxamine promotes mesenchymal stem cell homing in noise-induced injured cochlea through PI3K/AKT pathway[J].Cell Prolif,2018,51(2):e12434.
[12] 刘俊峰,莫秀梅,朱 璐,等.miRNA 与中医药免疫调控研究进展[J].陕西中医,2015,36(8):1101-1103.
[13] 郭晓安,施 磊.噪声性聋的治疗研究进展[J].医学综述,2019,25(5):978-983.
[14] 张自立,王金亮,孟祥朝.miRNA-183家族在恶性肿瘤中作用的研究[J].天津医药,2017,45(12):1337-1340.
[15] Weston MD,Tarang S,Pierce ML,et al.A mouse model of miR-96,miR-182 and miR-183 misexpression implicates miRNAs in cochlear cell fate and homeostasis[J].Sci Rep,2018,8(1):3569.
[16] Yang Z,Yao J,Cao X.Roles of the FGF signaling pathway in regulating inner ear development and hair cell regeneration[J].Yi Chuan,2018,40(7):515-524.
[17] 王园园,孙毓晗,柳 柯,等.中-低强度噪声暴露对耳蜗带状突触的影响[J].中华耳科学杂志,2019,17(2):203-208.
[18] 王晓燕,李兵兵.内耳毛细胞再生与修复的研究进展[J].中华耳鼻咽喉头颈外科杂志,2015,50(2):169-173.
[19] 李 根,薛 娜,宋 雷,等.中等强度带通噪声对C57小鼠内毛细胞形态与功能的影响[J].中华耳科学杂志,2019,17(2):159-164.
[20] 郭晓安,柯朝阳,柳 柯,等.100 dB SPL白噪声暴露对小鼠耳蜗听神经髓鞘的影响[J].中华耳科学杂志,2019,17(2):191-197.

备注/Memo

备注/Memo:
基金项目:四川省教育厅基金资助重点项目(18ZA0203)
更新日期/Last Update: 2021-05-06