[1]张荣臻,吕 超,石清兰,等.急性肝衰竭大鼠模型构建实验研究[J].陕西医学杂志,2021,50(3):268-271.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.003]
　ZHANG Rongzhen,LYU Chao,SHI Qinglan,et al.Construction of rat model of acute liver failure[J].,2021,50(3):268-271.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.003]
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急性肝衰竭大鼠模型构建实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年3期
页码:
268-271
栏目:
基础研究
出版日期:
2021-03-01

文章信息/Info

Title:
Construction of rat model of acute liver failure
作者:
张荣臻; 吕 超; 石清兰; 陈月桥; 黄雪霞; 毛德文
(广西中医药大学第一附属医院,广西 南宁 530023)
Author(s):
ZHANG Rongzhen; LYU Chao; SHI Qinglan; CHEN Yueqiao; HUANG Xuexia; MAO Dewen
(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)
关键词:
急性肝衰竭;  动物模型;  肝切除;  D-氨基半乳糖;  脂多糖;  大鼠
Keywords:
Acute liver failure;  Animal model;  Hepatectomy;  D-amino galactose;  Lipopolysaccharide;  Rat
分类号:
R 575.3
DOI:
DOI:10.3969/j.issn.1000-7377.2021.03.003
文献标志码:
A
摘要:
目的:探讨构建急性肝衰竭(ALF)大鼠模型的最佳方式。方法:将30只SPF级SD雄性大鼠随机分为对照组、手术组及模型组,每组10只。对照组常规饲养,不予手术处理。手术组采用单纯60%肝切除术。模型组行60%肝切除术后予以20 mg/100 g D-氨基半乳糖(D-GalN)和10 μg/100 g脂多糖(LPS)腹腔注射。观察造模过程中各组大鼠死亡情况。比较各组大鼠造模后24、48 h血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及总胆红素(TBIL)水平。通过HE染色观察各组大鼠造模后24、48 h肝组织病理学变化。结果:造模后48 h内,仅模型组大鼠非正常死亡3只,生存率为70%。造模后24、48 h,手术组与模型组大鼠ALT、AST、TBIL水平均高于对照组,模型组大鼠ALT、AST、TBIL水平均高于手术组(均P<0.05)。HE染色结果显示,模型组造模后24 h肝细胞呈灶状坏死,结构紊乱,可见肝细胞水肿; 造模后48 h肝细胞呈大块状坏死,结构紊乱,较对照组及手术组更符合肝衰竭模型要求。结论:60%肝切除术联合D-GalN及LPS腹腔注射操作简便,大鼠死亡率低,成模率高,为制作ALF大鼠模型的较理想方法。
Abstract:
Objective:To explore the best way to construct acute rat liver failure(ALF)model.Methods:30 SPF SD male rats were randomly divided into control group,operation group and model group,with 10 rats in each group.The normal control group was fed routinely without surgery.The rats in the operation group received 60% hepatectomy alone,and the rats in the model group received 20 mg/ 100 g D-GalN and 10 μg/100 g LPS intraperitoneal injection after 60% hepatectomy.The death of rats in each group was observed during modeling.The levels of serum ALT,AST and TBIL were compared at 24 and 48 hours after modeling.HE staining was used to observe the histopathological changes of the rats in each group at 24 and 48 hours after modeling.Results:Within 48 hours after modeling,only 3 rats in the model group died abnormally,and the survival rate was 70%.The levels of ALT,AST and TBIL in the operation group and model group were higher than those in the control group at 24 and 48 hours after modeling,and the levels of ALT,AST and TBIL in the model group were higher than those in the operation group(all P<0.05).He staining results showed that the hepatocytes in rats of the model group showed focal necrosis,structural disorder and hepatocyte edema at 24 hours after modeling,and massive necrosis,structural disorder at 48 hours after modeling,which was more consistent with the requirements of liver failure model than the normal group and the operation group.Conclusion:60% hepatectomy combined with D-GalN and LPS intraperitoneal injection has the advantages of simple operation,low mortality and high modeling rate,which is an ideal method for the establishment of rat model of ALF.

参考文献/References:

[1] 中华医学会感染病学分会肝衰竭与人工肝学组.肝衰竭诊治指南(2018版)[J].中华肝脏病杂志,2019,27(1):18-26.
[2] Bernal W,Auzinger G,Dhawan A,et al.Acute liver failure[J].Lancet,2010,376:190-201.
[3] Mannini BM,Silva SDA,Sankaramanivel S,et al.Useful properties of undifferentiated mesenchymal stromal cells and adipose tissue as the source in liver-regenerative therapy studied in an animal model of severe acute fulminant hepatitis[J].Cytotherapy,2015,17(8):1052-1065.
[4] 谭梅傲,方学君,曹 敏,等.青藤碱联合骨髓间充质干细胞预防Con A诱导大鼠免疫性肝损伤的实验研究[J].中国中西医结合杂志,2020,40(5):575-581.
[5] 刘江凯,刘香丽,李聪聪,等.LPS/D-GaLN诱导大鼠急性肝损伤动物模型的建立[J].中国急救医学,2019,39(2):175-178.
[6] 覃仁武,邱绍勤,陈赛专.苦参碱对硫代乙酰胺诱导的肝纤维化大鼠的影响[J].中成药,2020,42(3):764-767.
[7] 胡 昊,李 宁,宋圆圆,等.两种酒精灌胃方案诱导大鼠酒精性肝损伤模型的比较研究[J].医学理论与实践,2020,33(15):2420-2422.
[8] 章 波,檀燕君,麦婉婷,等.白背叶根水提取物对肝纤维化大鼠NF-κB信号通路的影响[J].中成药,2020,42(2):492-496.
[9] 李瑞祥,王红云,张 雷.大鼠肝脏在四氯化碳作用下NF-κB与CXCR1表达的研究[J].陕西医学杂志,2013,42(10):1305-1307,1438.
[10] 林建华,洪 重,周 斌.亮氨酸对大鼠肝部分切除术后肝再生的影响[J].温州医科大学学报,2019,49(10):753-755,759.
[11] Lentsch AB,Kato A,Yoshidome H,et al.Inflammatory mechanisms and therapeutic strategies for warm hepatic ischemia/reperfusion injury[J].Hepatology,2000,32(2):169-173.
[12] Tsung A,Klune JR,Zhang X,et al.HMGB1 release induced by liver ischemia involves Toll-like receptor 4 dependent reactive oxygen species production and calcium-mediated signaling[J].J Exp Med,2007,204(12):2913-2923.
[13] 龚建华,袁观斗,何松青.肝脏缺血再灌注损伤机制研究进展[J].中华实验外科杂志,2018,35(1):179-181.
[14] Yang F,Li X,Wang LK,et al.Inhibitions of NF-κB and TNF-α result in differential effects in rats with acute on chronic liver failure induced by D-Gal and LPS[J].Inflammation,2014,37(3):848-857.
[15] Lian LH,Wu YL,Wan Y,et al.Anti-apoptotic activity of gentiopicroside in D-galactosamine/lipopolysaccharide-induced murine fulminant hepatic failure[J].Chem Biol Interact,2010,188(1):127-133.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81560752,81960841); 广西壮族自治区自然科学基金资助面上项目(2018GXNSFAA281096); 广西壮族自治区科技计划项目(桂科AD17129001); 广西壮族自治区创新研究团队项目(2018GXNSFGA281002)
更新日期/Last Update: 2021-03-04