[1]张开亮,韩 康,张 勇,等.环状RNA hsa_circ_103809在骨肉瘤中的表达及生物学作用实验研究[J].陕西医学杂志,2021,50(3):259-263.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.001]
　ZHANG Kailiang,HAN Kang,ZHANG Yong,et al.Expression and biological effect of circular RNA hsa_circ_103809 in osteosarcoma[J].,2021,50(3):259-263.[doi:DOI:10.3969/j.issn.1000-7377.2021.03.001]
点击复制

环状RNA hsa_circ_103809在骨肉瘤中的表达及生物学作用实验研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年3期
页码:
259-263
栏目:
基础研究
出版日期:
2021-03-01

文章信息/Info

Title:
Expression and biological effect of circular RNA hsa_circ_103809 in osteosarcoma
作者:
张开亮1; 韩 康2; 张 勇3; 陈 明4; 关国锋5
(1.解放军第960医院泰安医疗区,山东 泰安 271000; 2.解放军第960医院济南医疗区,山东 济南 250000; 3.解放军联勤保障部队北戴河康复疗养中心,山东 秦皇岛 066100; 4.空军军医大学唐都医院骨科,陕西 西安 710038; 5.滨州医学院附属医院足踝外科,山东 滨州 256600)
Author(s):
ZHANG Kailiang; HAN Kang; ZHANG Yong; CHEN Ming; GUAN Guofeng
(Tai'an Medical District,the 960th Hospital of PLA,Tai'an 271000,China)
关键词:
环状RNA;  hsa_circ_103809;  骨肉瘤;  增殖;  侵袭;  迁移
Keywords:
Circular RNA;  Hsa_circ_103809;  Osteosarcoma;  Proliferation;  Invasion;  Migration
分类号:
R 738.1
DOI:
DOI:10.3969/j.issn.1000-7377.2021.03.001
文献标志码:
A
摘要:
目的:探索环状RNA hsa_circ_103809在骨肉瘤中的表达及生物学作用。方法:采用荧光定量PCR(qRT-PCR)检测骨肉瘤组织及细胞系中hsa_circ_103809表达水平。将骨肉瘤细胞分为两组,实验组转染hsa_circ_103809 siRNA序列,对照组转染siRNA阴性对照(NC)序列。采用CCK-8增殖实验和Transwell侵袭与迁移实验检测两组细胞的增殖、侵袭和迁移能力。分析骨肉瘤组织中hsa_circ_103809表达水平与骨肉瘤患者临床参数的相关性。结果:骨肉瘤组织hsa_circ_103809表达水平明显高于相应瘤旁组织,骨肉瘤细胞系hsa_circ_103809表达水平明显高于成骨细胞系hFOB1.19(均P<0.05)。转染后,实验组细胞hsa_circ_103809表达水平较对照组明显降低(P<0.01)。CCK-8实验结果显示,实验组细胞增殖能力较对照组明显减弱(P<0.01)。Transwell侵袭和迁移实验结果显示,实验组细胞侵袭和迁移能力均较对照组明显减弱(均P<0.01)。hsa_circ_103809表达水平与骨肉瘤患者临床分期及远处转移均呈正相关(均P<0.05)。结论:hsa_circ_103809高表达可促进骨肉瘤细胞增殖、侵袭和迁移。
Abstract:
Objective:To explore the expression and biological effect of circular RNA hsa_circ_103809 in osteosarcoma.Methods:qRT-PCR was used to detect the expression level of hsa_circ_103809 in osteosarocma tissues and cell lines.Osteosarcoma cells were divided into two groups.The experimental group was transfected with hsa_circ_103809 siRNA sequence.The control group was transfected with siRNA negative control(NC)sequence.CCK-8 and Transwell invasion and migration assays were performed to observe the abilities of proliferation,invasion and migration of the two groups.The correlation between hsa_circ_103809 expression level in osteosarcoma tissues and the clinical parameters of patients with osteosarcoma were analyzed.Results:Hsa_circ_103809 expression level in osteosarcoma tissues was higher than that in corresponding adjacent normal tissues,and the expression level of hsa_circ_103809 in osteosarcoma cell line was significantly higher than that in osteoblast cell line hFOB1.19(all P<0.05).After transfection,compared with the control group,the expression level of hsa_circ_103809 in the experimental group was significantly down-regulated,and the abilities of proliferation,invasion and migraiton were significantly weakened(all P<0.01).The expression level of hsa_circ_103809 was positively correlated with the clinical stage and distant metastasis of patients with osteosarcoma(all P<0.05).Conclusion:High expression of hsa_circ_103809 can promote the proliferation,invasion and migration of osteosarcoma cells.

参考文献/References:

[1] Misaghi A,Goldin A,Awad M,et al.Osteosarcoma:A comprehensive review[J].SICOT J,2018,4:12.
[2] Marina NM,Smeland S,Bielack SS,et al.Comparison of MAPIE versus MAP in patients with a poor response to preoperative chemotherapy for newly diagnosed high-grade osteosarcoma(EURAMOS-1):An open-label,international,randomised controlled trial[J].Lancet Oncol,2016,17(10):1396-1408.
[3] Davis LE,Bolejack V,Ryan CW,et al.Randomized double-blind phase Ⅱ study of regorafenib in patients with metastatic osteosarcoma[J].J Clin Oncol,2019,37(16):1424-1431.
[4] Patop IL,Kadener S.CircRNAs in cancer[J].Curr Opin Genet Dev,2018,48:121-127.
[5] Vo JN,Cieslik M,Zhang Y,et al.The landscape of circular RNA in cancer[J].Cell,2019,176(4):869-881.
[6] Wang C,Jing J,Cheng L.Emerging roles of non-coding RNAs in the pathogenesis,diagnosis and prognosis of osteosarcoma[J].Invest New Drugs,2018,36(6):1116-1132.
[7] Wang C,Ren M,Zhao X,et al.Emerging roles of circular RNAs in osteosarcoma[J].Med Sci Monit,2018,24:7043-7050.
[8] Huang SS,Guo WX,Ren MS.Circular RNA hsa_circ_103809 promotes cell migration and invasion of gastric cancer cells by binding to microRNA-101-3p[J].Eur Rev Med Pharmacol Sci,2020,24(11):6064-6071.
[9] Huang W,Lu Y,Wang F,et al.Circular RNA circRNA_103809 accelerates bladder cancer progression and enhances chemo-resistance by activation of miR-516a-5p/FBXL18 axis[J].Cancer Manag Res,2020,12:7561-7568.
[10] Qiu X,Wang Q,Song H,et al.Circ_103809 promotes breast cancer progression by regulating the PI3K/AKT signaling pathway[J].Oncol Lett,2020,19(6):3725-3730.
[11] Liu W,Ma W,Yuan Y,et al.Circular RNA hsa_circRNA_103809 promotes lung cancer progression via facilitating ZNF121-dependent MYC expression by sequestering miR-4302[J].Biochem Biophys Res Commun,2018,500(4):846-851.
[12] Zhan W,Liao X,Chen Z,et al.Circular RNA hsa_circRNA_103809 promoted hepatocellular carcinoma development by regulating miR-377-3p/FGFR1/ERK axis[J].J Cell Physiol,2020,235(2):1733-1745.
[13] Shaikh AB,Li F,Li M,et al.Present advances and future perspectives of molecular targeted therapy for osteosarcoma[J].Int J Mol Sci,2016,17(4):506.
[14] 彭 磊.骨肉瘤免疫治疗的研究现状及未来方向[J].陕西医学杂志,2000,29(4):221-223.
[15] 段大鹏,秦 静,王晓庆,等.Survivin、COX-2蛋白在骨肉瘤中的表达及其临床意义[J].陕西医学杂志,2015,44(10):1281-1283.
[16] Ji X,Shan L,Shen P,et al.Circular RNA circ_001621 promotes osteosarcoma cells proliferation and migration by sponging miR-578 and regulating VEGF expression[J].Cell Death Dis,2020,11(1):18.
[17] Yang J,Han Q,Li C,et al.Circular RNA circ_0001105 inhibits progression and metastasis of osteosarcoma by sponging miR-766 and activating YTHDF2 expression[J].Onco Targets Ther,2020,13:1723-1736.
[18] Gao AM,Yuan C,Hu AX,et al.Circ_ARF3 regulates the pathogeness of osteosarcoma by sponging miR-1299 to maintain CDK6 expression[J].Cell Signal,2020,72:109622.
[19] Yan M,Gao H,Lv Z,et al.Circular RNA PVT1 promotes metastasis via regulating of miR-526b/FOXC2 signals in OS cells[J].J Cell Mol Med,2020,24(10):5593-5604.

相似文献/References:

[1]周鹏翔,信 波△,宋晓鹏,等.CircAKT3对视网膜母细胞瘤细胞SO-Rb50增殖和凋亡的影响[J].陕西医学杂志,2020,49(8):932.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.005]
　ZHOU Pengxiang,XIN Bo,SONG Xiaopeng,et al.Effect of CircAKT3 on the proliferation and apoptosis of retinoblastoma SO-Rb50 cells[J].,2020,49(3):932.[doi:DOI:10.3969/j.issn.1000-7377.2020.08.005]
[2]宋 卿,张 蕾,刘 敏,等.环状RNA调控大肠癌侵袭转移机制研究进展*[J].陕西医学杂志,2020,49(12):1703.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.044]
　SONG Qing,ZHANG Lei,LIU Min,et al.Research progress on the mechanism of circular RNA regulating the invasion and metastasis of colorectal cancer[J].,2020,49(3):1703.[doi:DOI:10.3969/j.issn.1000-7377.2020.12.044]
[3]杨 旭,张致英,康龙丽,等.环状RNA与代谢性疾病研究进展[J].陕西医学杂志,2021,50(1):126.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.033]
　YANG Xu,ZHANG Zhiying,KANG Longli,et al.Advances in circular RNA and metabolic diseases[J].,2021,50(3):126.[doi:DOI:10.3969/j.issn.1000-7377.2021.01.033]
[4]徐梓凡,郑昊涵,谢田华,等.环状RNA功能及与眼科相关疾病关系研究进展[J].陕西医学杂志,2023,52(5):626.[doi:DOI:10.3969/j.issn.1000-7377.2023.05.030]
[5]任佳容,张 芸.环状RNA在心血管疾病中的研究进展[J].陕西医学杂志,2023,52(10):1446.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.038]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81702935)
更新日期/Last Update: 2021-03-04