[1]艾 林,高 鹏,李 恺,等.冷刺激磨牙对TRPM8基因敲除小鼠脑干中c-Fos表达的影响[J].陕西医学杂志,2021,50(2):135-138.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.002]
　AI Lin,GAO Peng,LI Kai,et al.Effect of cold stimulation of dental pulp on the expression of c-Fos in brainstem of TRPM8 knockout mice[J].,2021,50(2):135-138.[doi:DOI:10.3969/j.issn.1000-7377.2021.02.002]
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冷刺激磨牙对TRPM8基因敲除小鼠脑干中c-Fos表达的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
50
期数:
2021年2期
页码:
135-138
栏目:
基础研究
出版日期:
2021-02-05

文章信息/Info

Title:
Effect of cold stimulation of dental pulp on the expression of c-Fos in brainstem of TRPM8 knockout mice
作者:
艾 林1; 高 鹏1; 李 恺1; 张若冰2
(1.空军军医大学西京医院口腔科,陕西 西安 710032; 2.陕西省药品监督管理局,陕西 西安710065)
Author(s):
AI Lin; GAO Peng; LI Kai; ZHANG Ruobing
(Department of Stomatology,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
关键词:
冷刺激;  磨牙;  脑干;  基因敲除;  c-Fos表达
Keywords:
Cold stimulation;  Molar;  Brainstem;  Gene knockout;  c-Fos expression
分类号:
R 780
DOI:
DOI:10.3969/j.issn.1000-7377.2021.02.002
文献标志码:
A
摘要:
目的:研究冷刺激小鼠磨牙对TRPM8基因敲除小鼠脑干中c-Fos表达的影响。方法:麻醉野生型小鼠及TRPM8基因敲除小鼠后,冷刺激左上颌第一磨牙,灌注后组织制备,进行免疫组化研究及细胞计数,最后进行统计学分析。结果:同侧和对侧尾端核c-Fos表达差异无统计学意义(P>0.05),同侧和对侧间极核相比有统计学差异(P<0.05),在位于尾端核和间极核之间的过渡区,同侧的c-Fos表达也显著高于对侧(P<0.05); 免疫组织学切片显示实验侧c-Fos表达更强。TRPM8基因敲除小鼠与野生型小鼠出现相似的c-Fos表达模式,同侧c-Fos表达增加,但差异无统计学意义(P>0.05)。结论:c-Fos在三叉神经核中表达,确定冷刺激牙髓增加了野生型小鼠脑干中c-Fos的表达,TRPM8的缺失不会影响冷刺激诱导c-Fos的表达,为临床缓解牙髓炎等疼痛症状提供理论支持。
Abstract:
Objective:To study the effect of cold stimulation of dental pulp on the expression of c-Fos in brain stem of TRPM8 knockout mice.Methods:The left maxillary first molar of TRPM8 knockout mice After anesthetizing were stimulated with cold,and the tissue was prepared after perfusion.The immunohistochemical study and cell count were carried out.Finally,the statistical analysis was carried out.Results:No statistical significance was found in c-Fos expression on the ipsilateral side and contralateral side in nucleus caudalis(P>0.05).However,a significant increase in c-Fos expression was seen on the ipsilateral side versus the contralateral side in the nucleus interpolaris(P<0.05).In the transition zone,which was located between nucleus caudalis and nucleus interpolaris,a significant increase in c-Fos expression was also seen on the ipsilateral side than on the contralateral side(P<0.05).Immunohistological sections showed stronger expression of c-Fos on the experimental side.The expression pattern of c-Fos in TRPM8 knockout mice was similar to that of wild type mice,and the expression of c-Fos in ipsilateral was increased,but the difference was not significant(P>0.05).Conclusion:C-Fos is expressed in the trigeminal nucleus.It is determined that cold stimulation of dental pulp increases the expression of c-Fos in the brainstem of wild-type mice.The loss of TRPM8 does not affect the expression of c-Fos induced by cold stimulation,which provides theoretical guarantee in clinical relief for dental pulp pain symptoms such as inflammation.

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备注/Memo

备注/Memo:
基金项目:“十三五”军队后勤科研项目(CKJ20J027)
更新日期/Last Update: 2021-01-28