[1]肖 静,李文华,司晓盼,等.乙型肝炎HBV-M模式、前S2抗原与HBV-DNA定量检测关系探讨[J].陕西医学杂志,2020,49(11):1440-1443.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.018]
　XIAO Jing,LI Wenhua,SI Xiaopan,et al.Detection of HBV serum markers,and HBV-DNA quantitation Pre-S2 antigen in patients with HBV infection and their relationship[J].,2020,49(11):1440-1443.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.018]
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乙型肝炎HBV-M模式、前S2抗原与HBV-DNA定量检测关系探讨
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年11期
页码:
1440-1443
栏目:
临床研究
出版日期:
2020-11-05

文章信息/Info

Title:
Detection of HBV serum markers,and HBV-DNA quantitation Pre-S2 antigen in patients with HBV infection and their relationship
作者:
肖 静; 李文华; 司晓盼; 纪玉红; 朱国艳
辽宁省朝阳市第二医院(朝阳122000)
Author(s):
XIAO Jing; LI Wenhua; SI Xiaopan; et al.
Chaoyang Second Hospital,Liaoning Province(Chaoyang 122000)
关键词:
乙肝病毒感染;  乙肝病毒血清标志物;  HBV-DNA定量;  前S2抗原
Keywords:
HBV infection;  HBV serum marker;  HBV-DNA quantification;  Pre-S2 antigen
分类号:
R512.62
DOI:
DOI:10.3969/j.issn.1000-7377.2020.11.018
文献标志码:
A
摘要:
目的:探讨乙肝肝炎患者乙肝病毒血清学标志物(HBV-M)模式、前S2抗原(Pre-S2)与HBV-DNA定量检测的关系。方法:选择180例乙型病毒性肝炎患者,近期均未接受相关抗病毒治疗。采用双抗夹心酶联免疫吸附法(ELISA)检测乙肝病毒血清标志物水平和前S2抗原,采用荧光定量聚合酶链反应(qRT-PCR)检测血清HBV-DNA定量,对HBV-M检出模式、Pre-S2阳性率情况与HBV-DNA定量检测结果进行分析。结果:共检出14种HBV-M模式,其中大三阳、小三阳和其他HBV-M模式分别占比25.56%、46.11%、28.33%,不同HBV-M模式HBV-DNA、Pre-S2阳性率比较均有统计学差异(P<0.05); 107例HBV-DNA阳性患者中,HBsAg、Pre-S2阳性检出率为93.46%、95.33%,明显高于HBeAg阳性率 47.66%%(P<0.05),且随着HBV-DNA载量水平升高,HBeAg阳性率也明显升高(P<0.05); 不同HBV-DNA载量患者HBsAg、Pre-S2阳性率均明显高于HBeAg阳性率(P<0.05)。结论:HBV-M、HBV-DNA定量和Pre-S2检测结果紧密相关,联合检测能为HBV感染及病情评估提供充分依据。
Abstract:
Objective:To explore the relationship between serum markers(HBV-M)pattern,Pre-S2 antigen(Pre-S2)and quantitative detection of HBV-DNA in patients with hepatitis B hepatitis.Methods:180 patients with Hepatitis B were selected.None of them received antiviral therapy recently.Serum HBV markers and Pre-S2 antigen were detected by ELISA.Serum HBV-DNA was detected by fluorescence quantitative polymerase chain reaction(qRT-PCR).HBV-M detection pattern,HBV-DNA positive rate and load,Pre-S2 positive rate were analyzed.Results:A total of 14 HBV-M patterns were detected,of which the big three positives,small three positives and other HBV-M patterns accounted for 25.56%,46.11%,28.33%,respectively.The positive rates of HBV-DNA and Pre-S2 in different HBV-M patterns were statistically different(P<0.05).among the 107 HBV-DNA positive patients,the positive detection rates of HBsAg and Pre-S2 were 93.46% and 95.33%,which were significantly higher than the HBeAg positive rate of 47.66%%(P<0.05),and with the increase of HBV-DNA load level,the positive rate of HBeAg also increased significantly(P<0.05).The positive rates of HBsAg and Pre-S2 in patients with different HBV-DNA load were significantly higher than the positive rate of HBeAg(P<0.05).Conclusion:The results of HBV-M,HBV-DNA and PRE-S2 are closely related.The combined detection can provide sufficient evidence for HBV infection and disease evaluation.

参考文献/References:

[1] 龚甫哲,龚震宇.2016年全球乙型肝炎病毒感染者治疗情况[J].疾病监测,2018,33(11):93-94.
[2] 刘军辉,汪靖园,王林川,等.Pre-S1Ag和Pre-S2Ag及HBV-LP在乙肝病毒筛查中的价值[J].贵州医科大学学报,2018,43(12):78-82.
[3] 赵海燕,杨 东,洪 伟,等.慢性乙型肝炎、乙肝肝硬化患者中HBV-DNA水平、HBV-M、淋巴细胞亚型特点分析[J].广东医学,2019,40(3):432-435.
[4] 王贵强,王福生,成 军,等.慢性乙型肝炎防治指南(2015年版)[J].中华实验和临床感染病杂志:电子版,2015,19(5):1-18.
[5] 张国民,缪 宁,郑 徽,等.中国2005-2016年乙型病毒性肝炎报告发病的年龄和地区特征[J].中国疫苗和免疫,2018,24(2):121-126.
[6] 孙 菡,周新民,尹 芳,等.替比夫定治疗HBeAg阳性慢性乙型肝炎应答反应与HBeAg/HBsAg比值相关性研究[J].陕西医学杂志,2018,47(9):1137-1140.
[7] Brancaccio G,Fasano M,Grossi A,et al.Clinical outcomes in patients with hepatitis D,cirrhosis and persistent hepatitis B virus replication,and receiving long-term tenofovir or entecavir[J].Alimentary Pharmacology & Therapeutics,2019,49(8):1071-1076.
[8] 赵 棉,张 力,赵亚妮.HBVDNA定量与乙肝血清学标志物定量联合检测乙肝病毒感染的效果分析[J].实用临床医药杂志,2018,22(1):37-40.
[9] 雷香菊,陈 萍,何雪琴,等.乙肝病毒基因型、核苷(酸)耐药基因突变对肝硬化、肝癌的影响[J].国际检验医学杂志,2019,40(9):35-38,43.
[10] Hatazawa Y,Yano Y,Okada R,et al.Quasispecies variant of Pre-S/S gene in HBV-related hepatocellular carcinoma with HBs antigen positive and occult infection[J].Infectious Agents and Cancer,2018,13(1):7-13.
[11] 杨传信.PreS1抗原和HBV血清学标志物与HBV DNA的相关性及临床意义[J].临床输血与检验,2019,21(1):58-60.
[12] Chang YC,Fu LC,Chen WC,et al.Interaction of S17 antibody with the functional binding region of the Hepatitis B virus Pre-S2 epitope[J].Viral Immunology,2018:31(7):492-499.
[13] Teng CF,Shyu WC,Jeng LB.Abstract 3120:Tumor-infiltrating lymphocyte profiling in hepatitis B virus pre-S2 mutant-positive hepatocellular carcinoma[J].Cancer Research,2018,78(13):3120-3122.
[14] 赵娅南,肖 霞.乙型肝炎患者前S1及前S2抗原与HBV-DNA的检测结果的相关性分析[J].检验医学与临床,2018,15(14):2141-2143.
[15] 侯 玲,刘 韧,龙训琴,等.乙型病毒性肝炎患者血清HBV DNA与HBeAg、HBsAg的相关性分析[J].检验医学与临床,2018,15(7):936-939.
[16] Yen CJ,Ai YL,Tsai HW,et al.Hepatitis B virus surface gene Pre-S2 mutant as a high-risk serum marker for hepatoma recurrence after curative hepatic resection[J].Hepatology,2018,68(3):815-826.
[17] Dyah C,Amin M,Kholili U,et al.The analysis of mutation profile on pre-s1 and Pre-S2 region of hepatitis B virus in chronic liver disease[J].Malaysian Journal of Biochemistry and Molecular Biology,2018,21(3):85-92.
[18] 付 杰,杨国骏,蒋兴宇,等.3种酶联免疫吸附试验检测乙型肝炎病毒前S1及S2抗原的比较[J].国际检验医学杂志,2017,38(15):2017-2018.

更新日期/Last Update: 2020-11-05