[1]叶莉娜,张 乐.干扰素调控因子4对缺氧缺血性脑病新生大鼠炎症反应及血脑屏障功能的影响[J].陕西医学杂志,2020,49(11):1375-1378.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.002]
　YE Lina,ZHANG Le..Effect of IRF4 on inflammatory response and blood-brain barrier function in neonatal rats with hypoxic ischemic encephalopathy[J].,2020,49(11):1375-1378.[doi:DOI:10.3969/j.issn.1000-7377.2020.11.002]
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干扰素调控因子4对缺氧缺血性脑病新生大鼠炎症反应及血脑屏障功能的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年11期
页码:
1375-1378
栏目:
基础研究
出版日期:
2020-11-05

文章信息/Info

Title:
Effect of IRF4 on inflammatory response and blood-brain barrier function in neonatal rats with hypoxic ischemic encephalopathy
作者:
叶莉娜1; 张 乐2
1.陕西省渭南市妇幼保健院(渭南 714000); 2.内蒙古自治区乌兰察布市凉城县医院(乌兰察布 013750)
Author(s):
YE Lina; ZHANG Le.
Weinan Maternal and Child Health Hospital,Shaanxi Province(Weinan 714000)
关键词:
干扰素调控因子4;  新生大鼠;  缺氧缺血性脑病;  炎症;  血脑屏障
Keywords:
Interferon regulatory factor 4;  Neonatal rats;  Hypoxic-ischemic encephalopathy;  Inflammation;  Blood-brain barrier
分类号:
R722.19
DOI:
DOI:10.3969/j.issn.1000-7377.2020.11.002
文献标志码:
A
摘要:
目的:采用新生大鼠缺氧缺血性脑病(HIE)模型,研究干扰素调控因子4(IRF4)对HIE新生大鼠炎症反应和血脑屏障功能的影响,为临床应用IRF4治疗新生儿缺氧缺血性脑病提供实验依据。方法:选取10日龄健康雄性SD大鼠10只作为对照组,10只IRF4基因敲除SD大鼠用RVM法制作HIE模型作为IRF4KO组。HIE后7 d测定脑萎缩和行为缺陷,ELISA法测定血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)和白细胞介素-4(IL-4)水平,ELISA检测血清中基质金属蛋白酶-9(MMP-9)水平,免疫组织化学检测脑组织中MMP-9水平。结果:HIE后7 d,IRF4KO组新生大鼠较对照组脑萎缩严重,表现行为缺陷,且血清中促炎因子TNF-α、IL-1β水平增加,抑炎因子IL-10、IL-4水平下降,MMP-9水平显著升高,表明炎症反应增加,血脑屏障功能显著受损。结论:IRF4在新生大鼠HIE的神经炎症及血脑屏障功能中起调节作用,提示IRF4可能是新生儿缺血性脑损伤的潜在治疗靶点。
Abstract:
Objective:To study the effect of interferon regulatory factor 4(IRF4)on the inflammatory response and blood-brain barrier function of hypoxic-ischemic encephalopathy(HIE)neonatal rats by adopting a HIE model in neonatal rats,and to provide experimental evidence for clinical application of IRF4.Methods:Ten 10-day-old healthy male SD rats were selected as the control group,and 10 IRF4 gene knockout SD rats were used to make HIE models by RVM as the IRF4KO group.Brain atrophy and behavioral deficits were measured 7 days after HIE.Serum levels of cytokines such as TNF-α,IL-1β,IL-10,IL-4 and MMP-9 were determined by ELISA.MMP-9 levels in brain tissue was detected by immunohistochemical method.Results:7 days after HIE,neonatal rats in the IRF4KO group had more severe brain atrophy than the control group,exhibited behavioral defects,and serum pro-inflammatory factors TNF-α and IL-1β levels increased,and anti-inflammatory factors IL-10 and IL-4 levels decreased,and MMP-9 level significantly increased,which indicated that the inflammatory response increased and the BBB function was significantly impaired.Conclusion:IRF4 plays a regulatory role in neuroinflammation and BBB function,suggesting that IRF4 can serve as a potential therapeutic target for neonatal ischemic brain injury.

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更新日期/Last Update: 2020-11-05