[1]刘 斌,马原源,毛文静,等.偏头痛模型大鼠血浆一氧化氮和降钙素基因相关肽含量变化及氟桂利嗪干预效果实验研究*[J].陕西医学杂志,2020,49(7):771-773.[doi:DOI:10.3969/j.issn.10007377.2020.07.001]
　LIU Bin,MA Yuanyuan,MAO Wenjing,et al.Changes of nitric oxide and calcitonin gene-related peptide in plasma of migraine model rats and the intervention effect of flunarizine[J].,2020,49(7):771-773.[doi:DOI:10.3969/j.issn.10007377.2020.07.001]
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偏头痛模型大鼠血浆一氧化氮和降钙素基因相关肽含量变化及氟桂利嗪干预效果实验研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年7期
页码:
771-773
栏目:
基础研究
出版日期:
2020-07-05

文章信息/Info

Title:
Changes of nitric oxide and calcitonin gene-related peptide in plasma of migraine model rats and the intervention effect of flunarizine
作者:
刘 斌1; 马原源1; 毛文静1; 邓春颖1; 王彩霞1; 杨海霞2
1.华北理工大学附属医院神经内一科(唐山 063000); 2.河北省迁安市老干部局医院(迁安 064400)
Author(s):
LIU Bin; MA Yuanyuan; MAO Wenjing; et al.
Department of Neurology,Affiliated Hospital of North China University of Science and Technology(Tangshan 063000)
关键词:
偏头痛;  一氧化氮;  降钙素基因相关肽;  氟桂利嗪;  干预;  效果
Keywords:
Migraine;  Nitric oxide;  Calcitonin gene-related peptide;  Flunarizine;  Intervention;  Effect
分类号:
R747.2
DOI:
DOI:10.3969/j.issn.10007377.2020.07.001
文献标志码:
A
摘要:
目的:探讨偏头痛模型大鼠血浆一氧化氮(NO)和降钙素基因相关肽(CGRP)含量变化及氟桂利嗪干预效果。方法:采用硝酸甘油(GTN)制备偏头痛模型,实验大鼠36只随机分为生理盐水对照组(对照组)、偏头痛模型组(模型组)和氟桂利嗪干预组(氟桂利嗪组)。采用硝酸还原酶法测定NO含量和放射免疫法检测颈静脉血中CGRP含量。结果:与对照组比较,模型组两个时间点(3 h、6 h)血浆中NO和CGRP含量明显增加,差异有统计学意义(均P <0.01); 6 h组血浆中NO和CGRP含量少于3 h组,比较差异有统计学意义(均P <0.01)。与模型组比较,氟桂利嗪组两个时间点(3 h、6 h)血浆中NO和CGRP含量明显减少,差异有统计学意义(均P <0.01); 6 h组血浆中NO和CGRP含量少于3 h组,比较差异有统计学意义(均P <0.01)。 结论:NO和CGRP在偏头痛发病过程中起重要作用,氟桂利嗪可通过抑制NO、CGRP而发挥防治偏头痛的作用。
Abstract:
Objective:To investigate the changes of nitric oxide(NO)and calcitonin gene-related peptide(CGRP)levels in plasma of migraine model rats and the intervention effect of flunarizine. Methods:36 rats were randomly divided into normal saline control group(control group),migraine model group(model group)and flunarizine intervention group(flunarizine group). The migraine model was established by glyceryl trinitrate(GTN). The content of NO were determined by nitrate reductase method,and the content of CGRP in jugular blood of migraine model rats were determined by radioimmunoassay at 3 h and 6 h after modeling. Results:Compared with the control group,the contents of NO and CGRP in plasma of the model group increased significantly at two time points(3 h,6 h),and the difference was statistically significant(P<0.01). The contents of NO and CGRP in plasma of the 6-hour group were less than those in the 3-hour group,and the difference was statistically significant(all P<0.01). Compared with the model group,the contents of NO and CGRP in plasma of flunarizine group at two time points(3 h,6 h)were significantly decreased with statistical difference(P<0.01). The contents of NO and CGRP levels in plasma of the 6-hour group were less than those in the 3-hour group,and the difference was statistically significant(all P<0.01). Conclusions:NO and CGRP play an important role in the pathogenesis of migraine. Flunarizine can play a role in the treatment of migraine by inhibiting NO and CGRP.

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备注/Memo

备注/Memo:
*河北省卫生健康委员会重点科技研究计划项目(20150499)
更新日期/Last Update: 2020-07-28