[1]王亚平,彭春艳,朱名安Δ.冠心病合并代谢综合征患者固醇调节元件结合蛋白表达水平与脂代谢指标相关性研究*[J].陕西医学杂志,2020,49(4):419-423,438.
　WANG Yaping,PENG Chunyan,ZHU Mingan..Correlation between the levels of SREBPs and the indicators of lipid metabolism in patients with coronary heart disease complicated with metabolic syndrome[J].,2020,49(4):419-423,438.
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冠心病合并代谢综合征患者固醇调节元件结合蛋白表达水平与脂代谢指标相关性研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年4期
页码:
419-423,438
栏目:
临床研究
出版日期:
2020-04-05

文章信息/Info

Title:
Correlation between the levels of SREBPs and the indicators of lipid metabolism in patients with coronary heart disease complicated with metabolic syndrome
文章编号:
DOI:10.3969/j.issn.10007377.2020.04.008
作者:
王亚平1; 2; 彭春艳2; 朱名安3Δ
1.锦州医科大学研究生院(锦州121000); 2.湖北医药学院附属太和医院检验科(十堰442000); 3.湖北医药学院附属人民医院检验科(十堰 442000)
Author(s):
WANG Yaping; PENG Chunyan; ZHU Mingan.
Graduate School of Jinzhou Medical University(Jinzhou 121000)
关键词:
冠心病;  代谢综合征;  固醇调解元件结合蛋白;  脂代谢;  危险因素;  相关性
Keywords:
Coronary heart disease;  Metabolic syndrome;  Sterol regulatory element-binding proteins;  Lipid metabolism;  Risk factors;  Correlation
分类号:
R543.3
文献标志码:
A
摘要:
目的:固醇调节元件结合蛋白(SREBPs)是一种重要的转录因子,对调控哺乳动物的脂质稳态至关重要。本研究的目标是探讨冠心病(CAD)合并代谢综合征(MS)患者外周血白细胞SREBPs表达水平与脂代谢指标的相关性。方法:收取健康对照者159例,CAD患者70例,CAD合并MS患者73例。空腹抽取静脉血测定各组临床生化指标,实时荧光定量PCR检测外周血白细胞中SREBPs(SREBP-1,SREBP-2)的mRNA表达水平。结果:与健康对照组相比,CAD组与CAD合并MS组外周血白细胞SREBP-1 mRNA表达水平显著降低。在所有样本中,SREBP-1与总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A1(Apo-A1)、胱抑素-C(Cys-C)呈正相关,与高敏C反应蛋白质(hs-CRP)、肌酸激酶-MB(CK-MB)、同型半胱氨酸(HCY)呈负相关; 在疾病样本中,SREBP-1与HDL-C、GSP呈正相关,与HCY、hs-CRP呈负相关。Logistic回归分析显示,SREBP-1可能是CAD合并MS的潜在风险因素[优势比(OR)= 0.346,95%可信区间(CI)=(0.191,0.625),P <0.001]。ROC曲线分析显示,单独用SREBP-1预测CAD合并MS严重程度指标时,灵敏度较高但特异度较低; 联合HDL-C或Apo-A1预测诊断后,特异度明显升高但灵敏度较低。结论:SREBP-1可能成为诊断CAD合并MS的潜在生物学标志物,与脂代谢指标联合有更大的评估和诊断价值。
Abstract:
Objective:To investigate the correlation between the expression levels of SREBPs in peripheral blood leukocytes and lipid metabolism in patients with coronary heart disease(CAD)complicated with metabolic syndrome(MS). Methods:159 healthy controls(control group),70 CAD patients(CAD group)and 73 CAD patients with MS(CAD-MS group)were collected. Fasting venous blood was taken to determine the clinical biochemical indicators,and real-time fluorescence quantitative PCR was used to detect the mRNA expression levels of SREBPs(SREBP-1,SREBP-2)in peripheral blood leukocytes. Results:Compared with the control group,the expression levels of SREBP-1 mRNA in the peripheral blood of the CAD group and the CAD-MS group were significantly reduced. In all samples,SREBP-1 was positively correlated with TC,HDL-C,Apo-A1 and CysC,but negatively correlated with hs-CRP,CK-MB and HCY. In disease samples,SREBP-1 was positively correlated with HDL-C and GSP,but negatively correlated with hs-CRP and HCY. Logistic regression analysis showed that SREBP-1 might be a potential risk factor for CAD complicated with MS(OR=0.346,95%CI 0.191 to 0.625,P<0.001). ROC curve analysis showed that SREBP-1 alone had higher sensitivity but lower specificity in predicting the severity index of CAD combined with MS,while HDL-C or Apo-A1 combined with SREBP-1 had higher specificity but lower sensitivity.Conclusion:SREBP-1 may become a potential biomarker for the diagnosis of CAD complicated with MS,and the combined diag-nosis with HDL-C and Apo-A1 has greater evaluation and diagnostic value,but a larger sample size is needed to further verify the results of this experiment.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目(81600358)湖北省科技厅面上项目(2019CFB434)
更新日期/Last Update: 2020-04-13