[1]姚红月,李 岩,陈美雪,等.miR223p靶向负调控eIF4E对儿童骨肉瘤细胞增殖影响的研究[J].陕西医学杂志,2020,49(1):16-19.
　YAO Hongyue,LI Yan,CHEN Meixue,et al.Effect of miR223p targeting negatively regulated eIF4E on the proliferation of osteosarcoma cells in children[J].,2020,49(1):16-19.
点击复制

miR223p靶向负调控eIF4E对儿童骨肉瘤细胞增殖影响的研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
49
期数:
2020年1期
页码:
16-19
栏目:
基础研究
出版日期:
2020-01-05

文章信息/Info

Title:
Effect of miR223p targeting negatively regulated eIF4E on the proliferation of osteosarcoma cells in children
文章编号:
DOI:10.3969/j.issn.10007377.2020.01.004
作者:
姚红月1; 李 岩2; 陈美雪1; 王金凤1△
1.锦州医科大学附属第一医院儿科(锦州121000)；2.锦州医科大学附属第一医院普外科(锦州121000)
Author(s):
YAO Hongyue; LI Yan; CHEN Meixue; et al.
Department of Pediatrics, the First Affiliated Hospital of Jinzhou Medical University（Jinzhou 121000）
关键词:
骨肉瘤; miR223p; 真核细胞翻译起始因子4E; 细胞增殖; 细胞凋亡
Keywords:
Key words Osteosarcoma; MicroRNA223p; EIF4E; Cell proliferation; Cell apoptosis
分类号:
R738.1
文献标志码:
A
摘要:
摘 要 目的：观察微小RNA223p（miR223p）与真核细胞翻译起始因子4E（eIF4E）的靶向关系，分析其对骨肉瘤细胞增殖的影响。方法：选择骨肉瘤细胞系SAOS2和成骨细胞系OB3进行研究，采用双荧光素酶报告基因实验检测miR223p与eIF4E的结合情况。建立空白对照组、空载体转染组、miR223p转染组、miR223p和eIF4E共转染组，采用CCK8法检测细胞活性，采用Western Blot检测增殖细胞核抗原（PCNA）的表达。应用实时荧光定量PCR法（qPCR）检测miR223p在27例儿童骨肉瘤组织中的表达，应用免疫组化法检测组织中eIF4E和Ki67的表达。结果：骨肉瘤细胞系中miR223p的表达明显低于正常成骨细胞系。双荧光素酶报告基因实验显示miR223p能引起转染pGL3eIF4EWT细胞系中荧光素酶活性降低。与空白对照组及空载体转染组相比，miR223p转染组细胞活性下降，PCNA表达下降，miR223p和eIF4E共转染组能逆转上述表达水平。MiR223p在骨肉瘤的不同肿物最大径和病理分级分组的表达中差异有统计学意义（P＜0.05）。MiR223p 与eIF4E、miR223p与Ki67均具有负相关性，eIF4E与Ki67具有正相关性。结论：miR223p靶向负调控eIF4E调节骨肉瘤细胞的增殖。儿童骨肉瘤中miR223p低表达是肿瘤进展的重要危险因素。
Abstract:
Abstract Objective：To observe the targeting relationship between microRNA223p (miR223p) and eIF4E,and analyze the effect on the proliferation of osteosarcoma cells. Methods:Osteosarcoma cell line SAOS2 and osteoblast line OB3 were selected for research,and the binding of miR223p to eIF4E was detected by double luciferase reporter gene assay. The blank control group,empty vector transfection group,miR223p transfection group,miR223p and eIF4E cotransfection group were established. Cell activity was detected by CCK8 method. The expression of PCNA was detected by Western Blot method. The expression of miR223p was detected by realtime fluorescent quantitative PCR (qPCR) in 27 children with osteosarcoma,and the expressions of eIF4E and Ki67 were detected by immunohistochemistry. Results:The expression of miR223p was significantly lower in osteosarcoma cell line than that in normal osteoblasts cell line. The double luciferase reporter gene experiment showed that miR223p could reduce the luciferase activity in pGL3eIF4EWT cell line. Compared with the blank control group and the empty vector transfection group,the cell activity and PCNA expression decreased in miR223p transfection group,miR223p and eIF4E cotransfection group could reverse the above expression levels. The expression of miR223p had statistically significant differences in different tumor maximum diameter and pathological grading of osteosarcoma. Negative correlation was found between miR223p and eIF4E,which was also found between miR223p and Ki67. Positive correlation was found between eIF4E and Ki67. Conclusion:MiR223p can negatively regulate eIF4E to regulate proliferation of osteosarcoma cells. Low expression of miR223p is an important risk factor for progression of osteosarcoma in children.

参考文献/References:

[1] 杜利清,白剑强,王小春,等. 同源重组修复蛋白RAD51在骨肉瘤中的表达及其意义[J].陕西医学杂志，2010,39(11):14431444.
[2] 段大鹏,秦 静,王晓庆,等. Survivin、COX2蛋白在骨肉瘤中的表达及其临床意义[J].陕西医学杂志，2015,34(10):1113.
[3] Joseph B,Kumar RV,Champaka G,et al. Biological tailoring of adjuvant radiotherapy in head and neck and oral malignancies-The potential role of p53 and eIF4E as predictive parameters[J]. Indian J Cancer,2019,56(4):330334.
[4] Luo XF,Wu XJ,Wei X,et al. LncRNA ADPGKAS1 regulated cell proliferation,invasion,migration and apoptosis via targeting miR5423p in osteosarcoma[J]. Eur Rev Med Pharmacol Sci,2019,23(20): 87518760.
[5] Li C,Ni J,Liu YX,et al. Response of miRNA223p and miRNA1495p tofolate deficiency and the differential regulation of mthfr expression in normal and cancerous human hepatocytes[J]. PLoS One,2017,12(1): e0168049.
[6] Dong HX,Wang R,Jin XY,et al. LncRNA DGCR5 promotes lung adenocarcinoma(LUAD) progression via inhibiting hsamiR223p[J]. J Cell Physiol,2018,233(5): 41264136.
[7] Zhao L,Hu K,Cao J,et al. lncRNA miat functions as a ceRNA to upregulate sirt1 by sponging miR223p in HCC cellular senescence[J]. Aging(Albany NY),2019,11(17):70987122.
[8] Xu M,Tao Z,Wang S,et al. Suppression of oncogenic protein translation via targeting eukaryotic translation initiation factor 4E overcomes chemoresistance in nasopharyngeal carcinoma[J]. Biochem Biophys Res Commun,2019,512(4):902907.
[9] Cao B,Duan M,Xing Y,et al. OGlcNAc transferase activates stemlike cell potential in hepatocarcinoma through OGlcNAcylation of eukaryotic initiation factor 4E[J]. J Cell Mol Med,2019,23(4): 23842398.
[10] Lv KT,Liu Z,Feng J,et al. miR223p regulates cell proliferation and inhibits cell apoptosis through targeting the eif4ebp3 gene in human cervical squamous carcinoma cells[J]. Int J Med Sci,2018,15(2): 142152.
[11] 夏良平,曾宗渊,曾 敬,等. 喉鳞癌eIF4E及其ser209磷酸化蛋白表达[J]. 中国肿瘤,2004,13(3):171173.
[12] 胡继军,方向明,熊碧芳,等. 胃癌eIF4E和cyclin D1表达及其临床病理意义[J]. 肿瘤防治研究,2006,31(2):9799.
[13] 方海洋,李群星. eIF4E与胃癌[J]. 国际肿瘤学杂志,2009,36(4):291293.
[14] Chen J,Wu FX,Luo HL,et al. Berberine upregulates miR223p to suppress hepatocellular carcinoma cell proliferation by targeting Sp1[J]. Am J Transl Res,2016,8(11):49324941.
[15] Wang M,Chen B,Ru Z,et al. CircRNA circITCH suppresses papillary thyroid cancer progression through miR223p/CBL/βcatenin pathway[J]. Biochem Biophys Res Commun,2018,504(1): 283288.
[16] Li X,Wang S,Chen Y,et al. miR22 targets the 3’ UTR of HMGB1 and inhibits the HMGB1associated autophagy in osteosarcoma cells during chemotherapy[J]. Tumour Biol,2014,35(6):60216028.
[17] Bersani F,Lingua MF,Morena D,et al. Deep sequencing reveals a novel miR22 regulatory network with therapeutic potential in rhabdomyosarcoma[J]. Cancer Res,2016,76(20):60956106.

相似文献/References:

[1]张 哲,程漠松,庄 鹤,等.骨肉瘤细胞中miR-215-5p和eIF2a靶向关系及临床意义[J].陕西医学杂志,2021,50(7):886.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.031]
　ZHANG Zhe,CHENG Mosong,ZHUANG He,et al.Targeting relationship between miR-215-5p and eIF2a in osteosarcoma cells and its clinical significance[J].,2021,50(1):886.[doi:DOI:10.3969/j.issn.1000-7377.2021.07.031]
[2]张素冰,高 辉,赵滨滨,等.罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白/缺氧诱导因子1α信号通路的影响[J].陕西医学杂志,2023,52(6):666.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.007]
　ZHANG Subing,GAO Hui,ZHAO Binbin,et al.Effects of ropivacaine on proliferation,invasion and mTOR/HIF-1α signaling pathway of osteosarcoma cells[J].,2023,52(1):666.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.007]
[3]李 程,关贵超,李 兵,等.骨肉瘤组织锌-α2-糖蛋白1表达及其与患者预后关系[J].陕西医学杂志,2024,(12):1709.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.025]
　LI Cheng,GUAN Guichao,LI Bing,et al.Expression of AZGP1 in osteosarcoma and its relationship with prognosis[J].,2024,(1):1709.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.025]

更新日期/Last Update: 2020-02-17