[1]赵侠勇,,赵君杰,等.IL-10在大鼠蛛网膜下腔出血后早期脑损伤中的作用研究*[J].陕西医学杂志,2019,(6):696-701.
　ZHAO Xiayong,ZHAO Junjie,SONG Jinning,et al.The effect of IL-10 on early brain injury after subarachnoid hemorrhage in rats[J].,2019,(6):696-701.
点击复制

IL-10在大鼠蛛网膜下腔出血后早期脑损伤中的作用研究*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年6期
页码:
696-701
栏目:
基础研究
出版日期:
2019-06-05

文章信息/Info

Title:
The effect of IL-10 on early brain injury after subarachnoid hemorrhage in rats
文章编号:
DOI:〖HT5K〗10.3969/j.issn.1000-7377.2019.06.004
作者:
赵侠勇1;  2; 赵君杰1; 宋锦宁1△; 金 涛
1.西安交通大学第一附属医院神经外科(西安 710061)； 2.陕西省安康市中心医院麻醉科 (安康 725000);3.陕西省安康市中心医院神经外科 (安康 725000)
Author(s):
ZHAO Xiayong;  ZHAO Junjie;  SONG Jinning;  et al.
Department of Neurosurgery, the First Affiliated Hospital of Xi’an Jiaotong University（Xi’an 710061）
关键词:
蛛网膜下腔出血; 炎症反应; 小胶质细胞; 白细胞介素10; 早期脑损伤
Keywords:
Key words Subarachnoid hemorrhage; Inflammatory response;  Microglia;  IL-10;  Early brain injury
分类号:
R392.3
文献标志码:
A
摘要:
摘 要 〖HT5K〗目的: 探讨IL-10在大鼠蛛网膜下腔出血（SAH）后早期脑损伤中的表达及作用。方法: 选择雄性SD大鼠60只，将其随机分为假手术组、SAH 1 d组、SAH 3 d组、SAH 1 d+IL-10组、SAH 3 d+IL-10组，每组各12只。SAH模型通过颈内动脉穿刺法建立，IL-10在SAH建模后30min内通过立体定向侧脑室穿刺给予。利用免疫组化染色和Western blot检测SAH后IL-10的表达，通过尼氏染色和HE染色评估IL-10对SAH早期脑损伤的作用，通过Western blotting检测TNF-α和IL-1β的变化，通过免疫组化染色评估SAH后小胶质细胞变化，并对大鼠进行行为学评分。结果：SAH 1 d组大鼠脑组织Wills环周围蛛网膜下腔大量出血，HE染色可见皮层组织疏松，神经元肿胀、核变形边集；SAH 1 d组及SAH 3 d组IL-10表达比较无统计学差异（P= 0.5669，P=0.9398）。与假手术组对比，SAH 1 d和SAH 3 d组TNF-α及IL-1β水平明显升高（P＜0.0001）。SAH后小胶质细胞明显活化。IL-10可缓解SAH 1 d组神经元损伤，减少SAH 1 d组及SAH 3 d组TNF-α及IL-1β的表达（P＜0.0001），显著减少小胶质细胞活化。结论：炎症反应在SAH早期脑损伤中起重要作用，SAH后小胶质细胞迅速激活，并通过释放TNF-α、IL-1β等炎症因子造成损伤。IL-10可通过缓解小胶质细胞活化，减少炎症因子的释放，改善SAH后早期脑损伤。
Abstract:
Abstract Objective: To explore the expression and role of IL-10 in early brain injury after subarachnoid hemorrhage (SAH) in rats. Methods:Sixty SD male rats were randomly divided into sham group, SAH 1 d group, SAH 3 d group, SAH 1 d+IL-10 group, and SAH 3 d+ IL-10 group, 12 rats for each group. The SAH Model was established by an endovascular perforation technique. IL-10 was injected stereotactically into lateral ventricle. The injury after SAH was assessed by eosin hematoxylin staining (HE staining). The expression of IL-10 was detected by western blot and immunohistochemistry. The effect of IL-10 on early brain injury was assessed by HE staining and Nissl staining. The expression of TNF-α and IL-1β was detected by western blot. Microglia was labelled by Iba-1 immunohistochemistry. Results :There was obvious subarachnoid hemorrhage around wills ring in SAH 1 d group. Compared with sham group, tissue looseness, neuronal swelling and nuclear anomalies were obviously seen in SAH 1 d group. The expression of IL-10 didn’t change significantly compared with SAH 1 d group and SAH 3 d group (P= 0.5669，P=0.9398). Compared with sham group, the level of TNFα and IL-1β in SAH 1d group and SAH 3 d group were significantly increased (P＜0.0001). The microglia were also activated after SAH. Based on Nissl staining and HE staining, IL-10 alleviated injuries induced by SAH. IL-10 also decreased expression of TNF-α and IL-1β (P＜0.0001) and reduced microglial activation in SAH 1 d group and SAH 3 d group compared with SAH group.Conclusion: The expression of IL-10 is unchanged after SAH. Microglia are activated after SAH and release TNFα and IL-1β. IL-10 alleviates the inflammatory response and early brain injury after SAH.

参考文献/References:

[1] Abraham MK, Chang WW. Subarachnoid Hemorrhage [J]. Emerg Med Clin North Am，2016，34(4):901-916.
[2] Topkoru B, Egemen E, Solaroglu I, et al. Early brain injury or vasospasm? an overview of common mechanisms[J]. Current Drug Targets，2017,18(12):1424-1429.
[3] 宋锦宁, 安吉洋. 蛛网膜下腔出血后早期脑损伤及神经保护治疗的研究进展[J]. 西安交通大学学报：医学版,2013,34(6):695-703.
[4] Zheng VZ, Wong GKC. Neuroinflammation responses after subarachnoid hemorrhage: A review [J]. J Clin Neurosci,2017,42:7-11.
[5] Garcia JM, Stillings SA, Leclerc JL, et al. Role of interleukin-10 in acute brain injuries [J]. Front Neurol, 2017,8:244.
[6] 郭 丹,宋锦宁,尹倩雯.大鼠蛛网膜下腔出血后血清lincRNA-p21的变化水平研究[J].中华神经外科疾病研究杂志,2017,16(5):412-416.
[7] Chen JH, Ke KF, Lu JH, et al. Protection of TGF-beta1 against neuroinflammation and neurodegeneration in Abeta1-42-induced Alzheimer's disease model rats [J]. PloS one,2015,10(2):e0116549.
[8] Zhao YL, Zhao YH, Zhang M, et al. Inhibition of TLR4 signalling-induced inflammation attenuates secondary injury after diffuse axonal injury in rats [J]. Mediators Inflamm,2016,2016:4706915.
[9] Huang T, Zhao J, Guo D, et al. Curcumin mitigates axonal injury and neuronal cell apoptosis through the PERK/Nrf 2 signaling pathway following diffuse axonal injury[J]. Neuroreport, 2018,29(8):661-77.
[10] Macdonald RL, Higashida RT, Keller E, et al. Clazosentan, an endothelin receptor antagonist, in patients with aneurysmal subarachnoid haemorrhage undergoing surgical clipping: a randomised, double-blind, placebo-controlled phase 3 trial (CONSCIOUS-2) [J]. Lancet Neurol,2011,10(7):618-625.
[11] Luo Y, Lu J, Ruan W,et al, MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats[J]. Brain Research Bulletin,2019,146:320-326.
[12] Sozen T, Tsuchiyama R, Hasegawa Y, et al. Role of interleukin-1beta in early brain injury after subarachnoid hemorrhage in mice [J]. Stroke, 2009,40(7):2519-2525.
[13] Fouda AY, Kozak A, Alhusban A, et al. Anti-inflammatory IL-10 is upregulated in both hemispheres after experimental ischemic stroke: Hypertension blunts the response [J]. Exp Transl Stroke Med, 2013,5(1):12.
[14] Perez-de Puig I, Miro F, Salas-Perdomo A, et al. IL-10 deficiency exacerbates the brain inflammatory response to permanent ischemia without preventing resolution of the lesion [J]. J Cereb Blood Flow Metab,2013,33(12):1955-1966.
[15] Chen X, Duan XS, Xu LJ, et al. Interleukin-10 mediates the neuroprotection of hyperbaric oxygen therapy against traumatic brain injury in mice [J]. Neuroscience, 2014,266:235-243.
[16] Wang KW, Cho CL, Chen HJ, et al. Molecular biomarker of inflammatory response is associated with rebleeding in spontaneous intracerebral hemorrhage [J]. Eur Neurol, 2011,66(6):322-327.
[17] Kooijman E, Nijboer CH, van Velthoven CT, et al. Long-term functional consequences and ongoing cerebral inflammation after subarachnoid hemorrhage in the rat [J]. PloS One,2014,9(6):e90584.
[18] Hopkins SJ, McMahon CJ, Singh N, et al. Cerebrospinal fluid and plasma cytokines after subarachnoid haemorrhage: CSF interleukin-6 may be an early marker of infection [J]. J Neuroinflamm, 2012,9:255.
[19] 郑淑立,刘 洋,王 未,等.构建表达白细胞介素-10的胚胎神经干细胞的研究[J].陕西医学杂志,2014,43(11):1443-1445.

相似文献/References:

[1]韩 笑,单 伟△.黄连素通过调控炎症水平抑制老年大鼠心肌梗死后心室重构实验研究[J].陕西医学杂志,2020,49(1):7.
　HAN Xiao,SHAN Wei..Berberine inhibits ventricular remodeling after myocardial infarction in aged rats by regulating inflammation level[J].,2020,49(6):7.

备注/Memo

备注/Memo:
*陕西省自然科学基础研究计划面上项目(S2015YFJM2049）
更新日期/Last Update: 2019-06-20