[1]赵侠勇,,赵君杰,等.IL-10在大鼠蛛网膜下腔出血后早期脑损伤中的作用研究*[J].陕西医学杂志,2019,(6):696-701.
　ZHAO Xiayong,ZHAO Junjie,SONG Jinning,et al.The effect of IL-10 on early brain injury after subarachnoid hemorrhage in rats[J].,2019,(6):696-701.
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IL-10在大鼠蛛网膜下腔出血后早期脑损伤中的作用研究*

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年6期
页码:
696-701
栏目:
基础研究
出版日期:
2019-06-05

文章信息/Info

Title:
The effect of IL-10 on early brain injury after subarachnoid hemorrhage in rats
文章编号:
DOI:〖HT5K〗10.3969/j.issn.1000-7377.2019.06.004
作者:
赵侠勇1;  2; 赵君杰1; 宋锦宁1△; 金 涛
1.西安交通大学第一附属医院神经外科(西安 710061)； 2.陕西省安康市中心医院麻醉科 (安康 725000);3.陕西省安康市中心医院神经外科 (安康 725000)
Author(s):
ZHAO Xiayong;  ZHAO Junjie;  SONG Jinning;  et al.
Department of Neurosurgery, the First Affiliated Hospital of Xi’an Jiaotong University（Xi’an 710061）
关键词:
蛛网膜下腔出血; 炎症反应; 小胶质细胞; 白细胞介素10; 早期脑损伤
Keywords:
Key words Subarachnoid hemorrhage; Inflammatory response;  Microglia;  IL-10;  Early brain injury
分类号:
R392.3
文献标志码:
A
摘要:
摘 要 〖HT5K〗目的: 探讨IL-10在大鼠蛛网膜下腔出血（SAH）后早期脑损伤中的表达及作用。方法: 选择雄性SD大鼠60只，将其随机分为假手术组、SAH 1 d组、SAH 3 d组、SAH 1 d+IL-10组、SAH 3 d+IL-10组，每组各12只。SAH模型通过颈内动脉穿刺法建立，IL-10在SAH建模后30min内通过立体定向侧脑室穿刺给予。利用免疫组化染色和Western blot检测SAH后IL-10的表达，通过尼氏染色和HE染色评估IL-10对SAH早期脑损伤的作用，通过Western blotting检测TNF-α和IL-1β的变化，通过免疫组化染色评估SAH后小胶质细胞变化，并对大鼠进行行为学评分。结果：SAH 1 d组大鼠脑组织Wills环周围蛛网膜下腔大量出血，HE染色可见皮层组织疏松，神经元肿胀、核变形边集；SAH 1 d组及SAH 3 d组IL-10表达比较无统计学差异（P= 0.5669，P=0.9398）。与假手术组对比，SAH 1 d和SAH 3 d组TNF-α及IL-1β水平明显升高（P＜0.0001）。SAH后小胶质细胞明显活化。IL-10可缓解SAH 1 d组神经元损伤，减少SAH 1 d组及SAH 3 d组TNF-α及IL-1β的表达（P＜0.0001），显著减少小胶质细胞活化。结论：炎症反应在SAH早期脑损伤中起重要作用，SAH后小胶质细胞迅速激活，并通过释放TNF-α、IL-1β等炎症因子造成损伤。IL-10可通过缓解小胶质细胞活化，减少炎症因子的释放，改善SAH后早期脑损伤。
Abstract:
Abstract Objective: To explore the expression and role of IL-10 in early brain injury after subarachnoid hemorrhage (SAH) in rats. Methods:Sixty SD male rats were randomly divided into sham group, SAH 1 d group, SAH 3 d group, SAH 1 d+IL-10 group, and SAH 3 d+ IL-10 group, 12 rats for each group. The SAH Model was established by an endovascular perforation technique. IL-10 was injected stereotactically into lateral ventricle. The injury after SAH was assessed by eosin hematoxylin staining (HE staining). The expression of IL-10 was detected by western blot and immunohistochemistry. The effect of IL-10 on early brain injury was assessed by HE staining and Nissl staining. The expression of TNF-α and IL-1β was detected by western blot. Microglia was labelled by Iba-1 immunohistochemistry. Results :There was obvious subarachnoid hemorrhage around wills ring in SAH 1 d group. Compared with sham group, tissue looseness, neuronal swelling and nuclear anomalies were obviously seen in SAH 1 d group. The expression of IL-10 didn’t change significantly compared with SAH 1 d group and SAH 3 d group (P= 0.5669，P=0.9398). Compared with sham group, the level of TNFα and IL-1β in SAH 1d group and SAH 3 d group were significantly increased (P＜0.0001). The microglia were also activated after SAH. Based on Nissl staining and HE staining, IL-10 alleviated injuries induced by SAH. IL-10 also decreased expression of TNF-α and IL-1β (P＜0.0001) and reduced microglial activation in SAH 1 d group and SAH 3 d group compared with SAH group.Conclusion: The expression of IL-10 is unchanged after SAH. Microglia are activated after SAH and release TNFα and IL-1β. IL-10 alleviates the inflammatory response and early brain injury after SAH.

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备注/Memo

备注/Memo:
*陕西省自然科学基础研究计划面上项目(S2015YFJM2049）
更新日期/Last Update: 2019-06-20