[1]江茂琼,贾钰铭△,叶文静,等.MDSCs对肺癌小鼠CD8+T细胞及生存期的影响研究[J].陕西医学杂志,2019,(3):293-296.
　JIANG Maoqiong,JIA Yuming,LI Ting,et al.Effect of MDSCs on CD8 T cells and survival time of lung cancer mice[J].,2019,(3):293-296.
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MDSCs对肺癌小鼠CD8+T细胞及生存期的影响研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年3期
页码:
293-296
栏目:
基础研究
出版日期:
2019-03-05

文章信息/Info

Title:
Effect of MDSCs on CD8 T cells and survival time of lung cancer mice
文章编号:
DOI:10.3969/j.issn.10007377.2019.03.003
作者:
江茂琼; 贾钰铭△; 叶文静; 李 婷; 邱 野; 江 健; 江玉兰; 叶文静
四川省宜宾市第二人民医院肿瘤科（宜宾644000）
Author(s):
JIANG Maoqiong; JIA Yuming; LI Ting; et al.
The Second People’s Hospital of Yibin in Sichuan Province (Yibin 644000)
关键词:
MDSCs; 肺癌; CD8+T细胞; 生存期; 动物实验; 小鼠
Keywords:
Key words MDSCs; Lung cancer; CD8+T cells; Survival time; Animal experciment; Mice
分类号:
R392.3
文献标志码:
A
摘要:
摘 要 目的：探讨MDSCs对肺癌小鼠CD8+T细胞及生存期的影响。方法：选取C57小鼠15只行皮下接种LLC，采用随机数字表法将肿瘤大小符合规格的小鼠分为对照组和观察组，对照组小鼠尾静脉注射100μg兔IgG作为对照抗体，观察组小鼠尾静脉注射100μg Gr1删除抗体以删除荷瘤小鼠中的MDSC。采用流式细胞术（FCM）检测荷瘤小鼠外周血中MDSC和CD8+T细胞的变化，同时记录荷瘤小鼠肿瘤生长情况和生存期。结果：注射Gr1前两组荷瘤小鼠外周血中MDSC和CD8+T细胞比例无统计学差异（P＞0.05）；注射Gr1第3天，观察组MDSC比例为（1.58±0.30），显著低于对照组（38.35±3.22），CD8+T细胞比例为（9.47±0.83），显著高于对照组（4.23±0.47）；注射Gr1第10天，MDSC比例为（31.95±3.60），显著低于对照组（63.67±5.77），CD8+T细胞比例为（5.82±0.56），显著高于对照组（2.88±0.34），差异具有统计学意义（P＜0.05）。在荷瘤小鼠肿瘤生长25d左右，随着抗体的代谢消除，两组肿瘤大小差异无统计学意义（P＞0.05）。观察组小鼠生存率显著高于对照组，差异具有统计学意义（P＜0.05）。结论：高水平MDSCs能够降低CD8+水平，对生存期有较大影响，随着时间的延长，注射MDSCs抗体无法降低肿瘤生长速度。
Abstract:
Abstract objective:To investigate the effect of MDSCs on CD8+T Cells and survival time of lung cancer mice.Methods:15 case C57 mice were subcutaneously inoculated with LLC,Mice with tumor size were randomly divided into two groups: control group and observation group.In control group, 100μg rabbit IgG was injected into tail vein as control antibody.Mice in observation group were injected with 100μg Gr1 to delete antibody to remove MDSC from tumorbearing mice.The changes of MDSC and CD8+T cells in peripheral blood of tumorbearing mice were detected by flow cytometry.And Tumor growth and survival time were recorded in tumorbearing mice.Results:Before injection of Gr1，there was no significant difference in the proportion of MDSC and CD8 T cells in peripheral blood between the two groups (P＞0. 05).On the third day of injection of Gr1,The ratio of MDSC in the observation group was (1.58 ±0.30) ,which was significantly lower than that in the control group (38.35 ±3.22).The ratio of CD8 T cells was (9.47 ±0.83), which was significantly higher than that in the control group (4.23 ±0.47).On the 10th day of injection of Gr1,The MDSC ratio was (31.95 ±3.60), which was significantly lower than that in the control group (63.67 ±5.77).The ratio of CD8 T cells was (5.82 ±0.56), which was significantly higher than that in the control group (2.88 ±0.34), and the difference was statistically significant (P＜0. 05).After 25 days of tumor growth, there was no significant difference in tumor size between the two groups with the elimination of antibody metabolism (P＞0.05).The survival rate in the observation group was significantly higher than that in the control group (P＜0.05).Conclusion: high level of MDSCs can decrease the level of CD8+ and have a great influence on the survival time. With the extension of time, injection of MDSCs antibody can not reduce the growth rate of tumor.

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更新日期/Last Update: 2019-03-15