[1]于敬坤,韩学超,李明志,等.二甲双胍对Eca109食管癌细胞迁移能力的影响及机制研究*[J].陕西医学杂志,2019,(2):139-143，147.
　YU Jingkun,HAN Xuechao,LI Mingzhi,et al.The effect and mechanism of metformin on the migration ability of Eca109 esophageal carcinoma cells[J].,2019,(2):139-143，147.
点击复制

二甲双胍对Eca109食管癌细胞迁移能力的影响及机制研究*
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年2期
页码:
139-143，147
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
The effect and mechanism of metformin on the migration ability of Eca109 esophageal carcinoma cells
文章编号:
DOI: 10.3969/j.issn.10007377.2019.02.001
作者:
于敬坤1; 韩学超22; 李明志1; 随振阳1; 张 琪1; 王长友1
1.华北理工大学附属医院普外科（唐山 063000）;2 华北理工大学医学实验研究中心 （唐山 063000）
Author(s):
YU Jingkun ; HAN Xuechao ; LI Mingzhi; et al.
Department of General Surgery,  Affiliated Hospital of North China University of Science and Technology（Tangshan 063000） 
关键词:
食管肿瘤; 跨细胞迁移; 细胞增殖; 细胞侵袭; 二甲双胍; 分子药理作用机制
Keywords:
Key words Esophageal neoplasms; Transcellular cell migration; Cell proliferation; Cell invasion;  Metformin; Molecular mechanisms of pharmacological action
分类号:
R735.1
文献标志码:
A
摘要:
摘 要 目的：研究二甲双胍对Eca109食管癌细胞迁移能力的影响及其可能机制。方法：MTT实验观察在盐酸二甲双胍作用下Eca109食管癌细胞的增殖能力；划痕实验观察盐酸二甲双胍作用24、48 h时Eca109食管癌细胞的迁移能力；Western blotting观察细胞侵袭、迁移相关蛋白MMP2、MMP9及GLUT1、MMP14的表达。结果：盐酸二甲双胍可在一定程度上抑制Eca109食管癌细胞的迁移能力，并降低GLUT1、MMP14、MMP2、MMP9的表达（P＜0.05）。结论：二甲双胍可以降低Eca109食管癌细胞的迁移能力，可能是通过抑制GLUT1、MMP14、MMP2、MMP9通路而实现的。
Abstract:
Abstract Objective: To study the effect and mechanism of metformin on the migration ability of Eca109 esophageal carcinoma cells .Methods: MTT assay was used to observe the proliferation ability of Eca109 esophageal cancer cells under the action of metformin hydrochloride .The migration ability of Eca109 esophageal cancer cells at 24 h and 48 h was observed by scratch assay .Western blotting observed the expression of cell migration related protein like MMP2, MMP9 and protein GLUT1,MMP14.Results: Metformin hydrochloride can inhibit the migration ability of Eca109 esophageal cancer cells to a certain extent (P< 0.05). Metformin hydrochloride also can reduce the expression of GLUT1, MMP14, MMP2 and MMP9(P<0.05).Conclusion: Metformin can reduce the migration ability of Eca109 esophageal cancer cells, possibly by inhibiting the migration ability of GLUT1,MMP14 ,MMP2 and MMP9 pathway to reduce Eca109 esophageal cancer cells.

参考文献/References:

\[1\] 刘 梦.环境污染对肿瘤发病率的动态效应分析\[D\].武汉：华中科技大学,2015.
\[2\] 吴 菲,林国桢,张晋昕.我国恶性肿瘤发病现状及趋势\[J\].中国肿瘤,2012,21(2):8185.
\[3\] 吴琦玮,沈 榕,张 莉.超声微探头辅助内镜对食管癌早期筛查及癌前病变诊断价值分析\[J\].陕西医学杂志,2018,47(7)：886888.
\[4\] 王 俊,王丽丹,江启成.我国肿瘤患者生命质量评价研究现状及特点\[J\].中华疾病控制杂志,2017,21(4):422424.
\[5\] Kobayashi M,Kato K,Iwama H,et al. Antitumor effect of metformin in esophageal cancer：in vitro study\[J\].Int J Oncol,2013,42(2):517524.
\[6\] 李国仁,于世忠.中国继发性食管癌的现状和进展\[J\].中华胸部外科电子杂志,2018,5(1):5358.
\[7\] Garber AJ,Duncan TG,Goodman AM,et al.Efficacy of metformin in type II diabetes：results of a doubleblind ,placebocontrolled ,doseresponse trieal\[J\].Am J Med,1997,103:491497.
\[8\] DeFronzo RA,Goodman AM.Efficacy of metformin in patients with noninsulindependent diabetes mellitus:The Multicenter Metformin Study Group\[J\].N Engl Med,1995,333:541549.
\[9\] 中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)\[J\].中国糖尿病杂志,2014,22(2):附录.〖ZK)〗
\[10\] Ji L,Han P,Wang X,et al.A randomized clinical trial of the safe and efficacy of sitagliptin and metformin coadministered to Chinese patients with type 2 diabetes mellitus\[J\].J Diabetes Investig,2016,19:727736.
\[11\] Ji L,Li H,Guo X ,et al.Impact of beseline BMI on glycemin control and weight change with metformin monotherapy inchinese type 2 diabetes patients.phase IV openlabel trial\[J\]. PLoS One ,2013,8:e57222.
\[12\] Evans JM ,Donnelly LA ,EmslieSmith AM ,et al. Metformin and reduced risk of cancer in diabetic patients\[J\].Br Med J,2005,330:13041305.
\[13\] 母义明,纪立农,宁 光,等.二甲双胍临床应用专家共识(2016年版)\[J\].中国糖尿病杂志,2016,24(10)：871884.〖ZK)〗
\[14\] 姜 宁,路 平.二甲双胍抗肿瘤作用机制\[J\].新乡医学院学报,2016,33(2): 150152.
\[15\] Evans JM,Donnelly LA,Emsliesmith AM,et al.Metformin and reduced risk of cancer in diabetic patients\[J\]. BMJ, 2005,330( 7503) : 13041305.
\[16\] Bowker SL,Majumdars R,Veugelers P,et al.Increased cancerrelated mortality for patients with type 2 diabetes who use sulfonylureas or insulin［J］.Diabetes Care,2006,29( 2)：254258.
\[17\] Radenkovic S,Konjevic G,Jurisic V,et al. Values of MMP2 and MMP9 in tumor tissue of basallike breast cancer patients\[J\].Cell Biochem Biophysics,2014, 68(1):143152.
\[18\] Lu W,Xia YH,Qu JJ, et al. p21activated kinase 4 regulation of endometrial cancer cell migration and invasion involves the ERK1 /2 pathway mediated MMP2 secretion\[J\]. Neoplasma,2013,60( 5) : 493503.
\[19\] Chen YJ,Lee LY,Chao Y K,et al. DSG3 facilitates cancer cell growth and invasion through the DSG3plakoglobinTCF/LEFMyc /Cyclin D1 /MMP signaling pathway\[J\]. PLoS One,2013,8( 5) : e64088.
\[20\] Chen F,Bai J,Li W,et al. RUNX3 suppresses migration, invasion and angiogenesis of human renal cell carcinoma \[J\].PLoS One,2013,8(2):e56241.
\[21\] Yanal,Seikim.MTMMPs play pivotal roles in cancer dissemination\[J\]. Clin ExP Metastasis,2002,19(3):209225.
\[22\] Liao H,Wang ZQ ,Deng ZP, et al.Curcumin inhibits lung cancer invasion and metastasis by attenuating GLUT1/MT1MMP/MMP2 pathway\[J\].International Journal of Clinical and Experimental Medicine,2015,6(8)：894857.

相似文献/References:

[1]武阿丽,张亚飞,吴兵,等.胃镜下扩张联合黏膜下注射曲安奈德治疗食管癌术后吻合口顽固性良性狭窄临床研究[J].陕西医学杂志,2016,(10):1321.

备注/Memo

备注/Memo:
*〖JP3〗中国煤炭工业协会2017年度科学技术研究指导性计划项目
更新日期/Last Update: 2019-03-13