[1]王 蒙,王倩倩,孙连庆△.丹酚酸B对代谢记忆环境下RSC96细胞的保护作用*[J].陕西医学杂志,2019,(12):1587-1590.
　WANG Meng,WANG Qianqian,SUN Lianqing..The protective effects of salvianolic acid B on RSC96 cell in metabolic memory environment[J].,2019,(12):1587-1590.
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丹酚酸B对代谢记忆环境下RSC96细胞的保护作用*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年12期
页码:
1587-1590
栏目:
基础研究
出版日期:
2019-12-05

文章信息/Info

Title:
The protective effects of salvianolic acid B on RSC96 cell in metabolic memory environment
文章编号:
DOI:10.3969/j.issn.10007377.2019.12.003
作者:
王 蒙1;  王倩倩2;  孙连庆2△
1西安交通大学（西安 710049）；2西安交通大学第一附属医院（西安 710061）
Author(s):
WANG Meng; WANG Qianqian; SUN Lianqing.
Xi’an Jiaotong University（Xi’an 710049）
关键词:
凋亡; 炎症; RSC96; 代谢记忆; 丹酚酸B; 活性氧
Keywords:
Key words Apoptosis; Inflammatory; RSC96; Metabolic memory; Salvianolic acid B; Reactive oxygen species
分类号:
R3925
文献标志码:
A
摘要:
摘 要 目的：探讨丹酚酸B对代谢记忆环境下离体培养的RSC96细胞凋亡及炎性因子变化规律的影响。方法：RSC96细胞分别培养于低糖培养组(56 mmol/L)、代谢记忆(125 mmol/L高糖培养48 h，56 mmol/L低糖培养24 h)、丹酚酸B组（125 mmol/L高糖+1 μmol/L丹酚酸B培养48 h，56 mmol/L低糖培养基+1 μmol/L丹酚酸B培养24h）中，流式法检测ROS含量，Western blot法检测Cleavedcaspase 9，Cleavedcaspase 3，P65，IL1β的表达。结果：代谢记忆环境可下调ROS含量，下调Cleavedcaspase 9，Cleavedcaspase 3,P65，IL1β等蛋白的表达；丹酚酸B可上调ROS含量，下调Cleavedcaspase 9，Cleavedcaspase 3，P65，IL1β等蛋白的表达。结论：代谢记忆环境下，丹酚酸B可能通过下调ROS引起细胞凋亡及炎性损伤保护RSC96细胞。
Abstract:
Abstract Objective:To observe the effect of Sal B on apoptosis and inflammatory of RSC96 cultured in metabolic memory environmentMethods:RSC96 cells were respectively cultured in control group(56mmol/L),metabolic memory(125mmol/L high glucose for 48h plus 56mmol/L glucose for 24 h) or Sal B group(125mmol/L high glucose + 1μmol/L Sal B for 48h plus 56mmol/L glucose+ 1μmol/L Sal B for 24 h)Flow cytometry was performed to detect ROS levelWestern blot was manipulated to detect the expression of Cleavedcaspase 9，Cleavedcaspase 3，P65，IL1βResults:The metabolic memory group ROS level was upregulated compared with the control groupHowever,Sal B group ROS level was downregulated compared with the metabolic memory groupThe expression of Cleavedcaspase 9，Cleavedcaspase 3，P65，IL1β in metabolic memory group decreased compared with the control groupHowever,the expression of Cleavedcaspase 9，Cleavedcaspase 3，P65，IL1β in Sal B decreased compared with the metabolic memory groupConclusion:Sal B protected RSC96 cells against apoptosis and inflammatory by eliminating ROS in metabolic memory environment

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目（81673785） 陕西省自然科学基金资助重点项目（2015JZ023）
更新日期/Last Update: 2019-12-19