[1]何梅俊,陈霜霜,任晶红,等.微小RNA125b5p在氧化应激损伤中的作用实验研究*[J].陕西医学杂志,2019,(12):1579-1583.
　HE Meijun,CHEN Shuangshuang,REN Jinghong,et al.The role of MicroRNA125b5p in H2O2induced oxidative stress[J].,2019,(12):1579-1583.
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微小RNA125b5p在氧化应激损伤中的作用实验研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年12期
页码:
1579-1583
栏目:
基础研究
出版日期:
2019-12-05

文章信息/Info

Title:
The role of MicroRNA125b5p in H2O2induced oxidative stress
文章编号:
DOI:10.3969/j.issn.10007377.2019.12.001
作者:
何梅俊; 陈霜霜; 任晶红; 贾 佳△
苏州大学药学院（苏州 215123）
Author(s):
HE Meijun; CHEN Shuangshuang; REN Jinghong; et al
College of Pharmaceutical Sciences,Suzhou University (Suzhou 215123)
关键词:
miR125b5p; 神经元细胞; 氧化应激; 过氧化氢; 抗氧化酶HO1
Keywords:
Key words MiR125b5p; Neuronal cells; Oxidative stress; Hydrogen peroxide; AntiOxidant enzymes heme oxygenase1
分类号:
R3923
文献标志码:
A
摘要:
摘 要 目的：利用体外神经元模型，探讨miR125b5p对氧化应激的影响。方法：建立过氧化氢（H2O2）诱导神经元细胞氧化应激模型，将miR125b5p的类似物（mimics）转染至HT22细胞实现其过表达。造体外氧化应激模型，采用 MTT（噻唑蓝）法检测细胞活力，实时定量 PCR（qPCR）检测miR125b5p表达水平及抗氧化酶HO1、Mn SOD的基因表达水平。分析微小RNA125b5p对氧化应激损伤的影响。结果：在体外氧化应激模型中miR125b5p表达量明显下降；转染miR125b5p的类似物（miR125b5p mimics）后，和阴性对照（NC）相比，miR125b5p在HT22细胞中过表达显著增加；MTT结果显示，miR125b5p mimics组与对照组相比细胞存活率明显增加；qPCR 结果显示，与NC组相比，miR125b5p过表达可上调抗氧化酶HO1和Mn SOD mRNA表达水平，发挥抗氧化作用。结论：miR125b5p在H2O2刺激的神经元细胞中明显下调，miR125b5p过表达可显著提高神经元细胞存活率。miR125b5p抗过氧化氢诱导的神经元氧化损伤的作用机制可能与提高抗氧化酶HO1、Mn SOD mRNA的表达相关。
Abstract:
Abstract Objective:We explored the role of miR125b5p in oxidative stress by using in vitro H2O2induced oxidative stress model in HT22 neuronal cellsMethods:The HT22 neuronal cells were cultured in vitroThe HT22 cells were treated with hydrogen peroxide (H2O2) for 24 hoursThe expression of miR125b5p and the expression of antioxidant enzymes heme oxygenase1 (HO1) and Manganese superoxide dismutase(Mn SOD) were analyzed with qRTPCRCell viability was determined with MTT assayResults:MiR125b5p levels were significantly decreased in H2O2 treated HT22 cellsOverexpression of miR125b5p mimics significantly increased cell viability after H2O2 treatmentIn addition,compared to control group,overexpression of miR125b5p mimics increased the mRNA expression levels of the antioxidant enzymes HO1 and Mn SOD in H2O2 treated HT22 cellsConclusion:miR125b5p was downregulated in neuronal cells following oxidative stressOverexpression of miR125b5p protected neuronal cells against oxidative stress by upregulation of antioxidant enzymes HO1 and Mn SOD expression

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目（81571124）
更新日期/Last Update: 2019-12-19