[1]甘昭平,梁亚萍,赵涛△,等.等位基因多重PCR技术快速诊断耐药结核病的应用研究*[J].陕西医学杂志,2018,(11):1438-1440,1444.
　Gan Zhaoping,Liang Yaping,Zhao Tao,et al.Application of allele multiple PCR in rapid diagnosis of drug-resistant tuberculosis[J].,2018,(11):1438-1440,1444.
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等位基因多重PCR技术快速诊断耐药结核病的应用研究*
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2018年11期
页码:
1438-1440,1444
栏目:
临床研究
出版日期:
2018-11-29

文章信息/Info

Title:
Application of allele multiple PCR in rapid diagnosis of drug-resistant tuberculosis
文章编号:
0.3969/j.issn.1000-7377.2018.11.021
作者:
甘昭平; 梁亚萍; 赵涛△; 王卓; 李静; 邹远妩
陕西省结核病防治院（西安 710100）
Author(s):
Gan Zhaoping; Liang Yaping; Zhao Tao; et al.
Shaanxi Provincial Gan Zhaoping Tuberculosis Control and Prevention Hospital (Xi’an 710100)
关键词:
结核分枝杆菌结核;  抗多种药物性广泛耐药结核@MAS-PCR
Keywords:
Mycobacterium tuberculosisTuberculosis; uultidrug-resistantExtensively drug-resistant tuberculosis@MAS-PCR
分类号:
R521
文献标志码:
A
摘要:
目的：探讨MAS-PCR技术作为分子生物学方法对耐多药结核病（MDR-TB）与广泛耐药结核病（XDR-TB）快速诊断的临床实用价值，为MDR-TB与XDR-TB的诊断和监测提供新的技术手段。方法：利用MAS-PCR技术对108株结核分枝杆菌临床分离株（含MDR-TB、XDR-TB敏感菌株）进行检测验证，并与传统比例法药敏试验结果进行比较，评价MAS-PCR技术的实用性。结果：对108株临床分离株（36株MDR，其中7株XDR）进行了MAS-PCR检测。MAS-PCR与比例法药敏试验比较，异烟肼耐药的敏感性、特异性、准确性、阳性预测值、阴性预测值分别为75.0%（36/48）、100%(57/57)、86.1%(93/108)、100.0%(36/36)、82.6%(57/69)；利福平耐药的敏感性、特异性、准确性、阳性预测值、阴性预测值分别为76.4%（42/55）、100%(53/53)、88.0%(95/108)、100.0%(42/42)、80.3%(53/66)；卡那霉素耐药的敏感性、特异性、准确性、阳性预测值、阴性预测值分别为100.0%(11/12)、100.0%(96/96)、99.0%(107/108)、100.0%(11/11)、99.0%(96/97)；氧氟沙星耐药的敏感性、特异性、准确性、阳性预测值、阴性预测值分别为75.0%（15/20）、98.9%（87/88）、94.4%（102/108）、93.8%（15/16）、94.6%（87/62）；卷曲霉素耐药的敏感性、特异性、准确性、阳性预测值、阴性预测值分别为77.8%（7/9）、99.0%（98/99）、97.2%(105/108)、87.5%(7/8)、98.0%(98/100)。结论：MAS-PCR技术检测MDR-TB及XDR-TB耐药性简便、快速、准确性、特异性高，对MDR-TB及XDR-TB耐药早期诊断具有重要意义。
Abstract:
Objective：To discuss the clinical value of MAS-PCR as a molecular biological method for the rapid diagnosis of MDR-TB and XDR-TB, and to provide a new technical means for the diagnosis and detection of MDR-TB and XDR-TB.Methods:Testing 108 strains of mycobacterium tuberculosis clinical isolates from our hospital (including MDR-TB, XDR-TB, sensitive strains)with MAS-PCR technology,and comparing with the result of drug sensitivity by traditional proportional method,we evaluate the practicality of MAS-PCR technology.Results：108 clinical isolates (36 MDR,7 XDR) were detected by MAS-PCR. Compared with the drug sensitivity test of MAS-PCR and proportional method,the rates of the sensitivity, specificity, accuracy and positive predictive values of isoniazide resistance were 75.0% (36/48), 100%(57/57),86.1%(93/108),100.0%(36/36), and 82.6%(57/69),respectively. the rates of the sensitivity, specificity,accuracy, positive predictive value and negative predictive value of rifampicin resistance were 76.4% (42/55),100%(53/53),88.0%(95/108),100.0%(42/42),80.3%(53/66), respectively.The rates of the sensitivity,specificity,accuracy,positive predictive value,negative predictive value of kanamycin resistance were 100.0%(11/12),100.0%(96/96),99.0%(107/108),100.0%(11/11), 99.0(96/97). the rates of the sensitivity, specificity, accuracy, positive predictive value of ofloxacin resistance were 75.0% (15/20), 98.9% (87/88),94.4% (102/108),93.8% (15/16),and 94.6% (87/62),respectively. the rates of the sensitivity,specificity,accuracy, positive predictive value and negative predictive value of capreomycin resistance were 77.8%(7/9),99.0%(98/99),97.2%(105/108),87.5%(7/8) and 98.0%(98/100), respectively. Conclusion：The detection of MDR-TB and XDR-TB resistance by MAS-PCR is simple, rapid, accurate and specific, and is of great significance to the early diagnosis of MDR-TB and XDR-TB resistance.

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备注/Memo

备注/Memo:
*陕西省卫生科研项目（2014D9）
更新日期/Last Update: 2018-11-30